摘要
本研究从常春藤(Hedera helix)中提取天然生物活性物质常春藤皂苷元(hederagenin, HG),探讨常春藤皂苷元对运动诱导骨骼肌损伤(EIMI)的保护作用及其可能机制。采用大鼠EIMI模型,给予不同剂量HG干预,并设置铁死亡诱导剂Erastin联合干预组。结果显示,与EIMI组相比,HG可显著降低血清肌酸激酶(CK)和肌酸激酶同工酶(CK-MM)水平[分别由(1 911.45±211.31) U/L和(1 485.65±144.37) U/L降至(684.70±85.53) U/L和(572.92±61.27) U/L, P<0.05],提高骨骼肌ATP含量[由(0.31±0.05)μmol/g提高至(0.86±0.14)μmol/g, P<0.05],并降低血清IL-1β、IL-6和TNF-α水平。此外,HG显著减少活性氧(ROS)和丙二醛(MDA)水平(分别降低约51.5%和56.1%, P<0.05),同时增加谷胱甘肽(GSH)含量[由(24.82±2.34) nmol/mg prot提高至(39.14±2.99) nmol/mg prot, P<0.05]。进一步分析铁稳态相关指标,HG可显著降低骨骼肌Fe2+含量[由(336.35±26.59)μmol/mg prot降至(184.78±17.17)μmol/mg prot, P<0.05],并上调GPX4、FTH1和FPN1的mRNA及蛋白表达水平。Erastin可逆转HG的保护作用,表现在CK、炎症因子、Fe2+水平升高,ATP、GSH、GPX4、FTH1和FPN1表达下降,进一步证实HG通过抑制铁死亡缓解EIMI。综上所述,HG可通过抗炎、抗氧化和调控铁稳态,抑制铁死亡,减轻EIMI相关的病理损伤,提示HG可能是一种新型运动损伤保护剂,为EIMI的防治提供新的策略。
This study extracted the natural bioactive compound hederagenin(HG)from Hedera helix and investi-gated its protective effects and potential mechanisms in exercise-induced skeletal muscle injury(EIMI).A rat mod-el of EIMI was established,and different doses of HG were administered,with an additional intervention group treated with the ferroptosis inducer Erastin.The results showed that,compared to the EIMI group,HG significantly reduced serum creatine kinase(CK)and creatine kinase-MM(CK-MM)levels[from(1911.45±211.31)U/L and(1485.65±144.37)U/L to(684.70±85.53)U/L and(572.92±61.27)U/L,P<0.05],increased ATP content in skeletal muscle[from(0.31±0.05)μmol/g to(0.86±0.14)μmol/g,P<0.05],and decreased serum IL-1β,IL-6,and TNF-αlevels.Additionally,HG significantly reduced reactive oxygen species(ROS)and malondialdehyde(MDA)levels(by approximately 51.5%and 56.1%,P<0.05),while increasing glutathione(GSH)content[from(24.82±2.34)nmol/mg prot to(39.14±2.99)nmol/mg prot,P<0.05].Further analysis of iron homeostasis-related markers re-vealed that HG significantly decreased Fe^(2+)levels in skeletal muscle[from(336.35±26.59)μmol/mg prot to(184.78±17.17)μmol/mg prot,P<0.05]and upregulated GPX4,FTH1,and FPN1 mRNA and protein expression.Erastin re-versed the protective effects of HG,as evidenced by increased CK,inflammatory cytokines,and Fe2+levels,along with decreased ATP,GSH,GPX4,FTH1,and FPN1 expression.These findings further confirmed that HG allevi-ates EIMI by inhibiting ferroptosis.In conclusion,HG exerts protective effects against EIMI by reducing inflam-mation,oxidative stress,and regulating iron homeostasis,thereby suppressing ferroptosis and mitigating muscle in-jury.This study suggests that HG may serve as a novel protective agent for managing exercise-induced muscle in-jury and provides new insights into potential therapeutic strategies.
作者
李彦桦
刘宝军
Li Yanhua;Liu Baojun(Department of Physical Education,Guilin University of Electronic Technology,Guilin,541001)
出处
《分子植物育种》
北大核心
2025年第11期3787-3795,共9页
Molecular Plant Breeding
基金
2024年广西高等教育本科教学改革工程项目(2024JGB189)资助。
