tone fruits, also known as drupes, have evolved an extremely hard wood-like shell called a stone to protect the seeds. Recently, the market value of stoneless cultivars has risen dramatically, which highlights the nee...tone fruits, also known as drupes, have evolved an extremely hard wood-like shell called a stone to protect the seeds. Recently, the market value of stoneless cultivars has risen dramatically, which highlights the needto cultivate stoneless fruit. Therefore, determining the underlying mechanism of fruit stone development isurgently needed. By employing the stone-containing jujube cultivar 'Youhe' and two stoneless Chinese jujube cultivars, 'Wuhefeng' and 'Daguowuhe', we comprehensively studied the mechanism of fruit stone development in jujube. Anatomical analysis and lignin staining revealed that the stone cultivar 'Youhe' jujube exhibited much greater lignin accumulation in the endocarp than the two stoneless cultivars. Lignin accumulation may be the key factor in fruit stone formation. By analyzing the transcriptome data and identifying differentially expressed genes(DEGs), 49 overlapping DEGs were identified in the comparisons of 'Youhe' jujube vs. 'Wuhefeng' jujube and 'Youhe' jujube vs. 'Daguowuhe' jujube. ZjF6H1-3 and ZjPOD, which are involved in lignin synthesis, were identified among these DEGs. The overexpression and silencing of ZjF6H1-3 and ZjPOD in wild jujube seedlings further confirmed their roles in lignin synthesis. In addition, two bHLH transcription factors were included in the 49 overlapping DEGs, and bHLH transcription factor binding motifs were found in the promoters of ZjF6H1-3 and ZjPOD, indicating that bHLH transcription factors are also involved in lignin synthesis and stone formation in Chinese jujube. This study provides new insights into the molecular networks underlying fruit stone formation and can serve as an important reference for the molecular design and breeding of stoneless fruit cultivars of jujube and fruit trees.展开更多
The effects of different treatments on cross compatibility in distant hybridization werestudied in 69 cross combinations among 9 cultivars of stone fruits. The results showedthat the setting percentage in balloon stag...The effects of different treatments on cross compatibility in distant hybridization werestudied in 69 cross combinations among 9 cultivars of stone fruits. The results showedthat the setting percentage in balloon stage was obviously higher than that in earlyblooming stage. The differences of fruit set between positive and reverse crosses weresignificant. Female parent affected cross compatibility more remarkably than male parentin distant hybridization. The appropriate electrostatic field, He-Ne laser combinedtreatment with 60Co γ-ray and He-Ne laser could improve the pollen germination rate invitro effectively, the fruit set percentage was higher than control, while 60Co γ-raytreatment reduced the pollen germination rate in vitro, and the fruit set percentageobtained by distant hybridization was lower than control.展开更多
<span>Total crop load has an inverse relationship with fruit size, while larger fruit size is often demanded by the peach and nectarine markets. Hand-thinning is extremely expensive, and thus, crop load adjustme...<span>Total crop load has an inverse relationship with fruit size, while larger fruit size is often demanded by the peach and nectarine markets. Hand-thinning is extremely expensive, and thus, crop load adjustment, using blossom thinners is a crucial practice to ensure production of commercially acceptable fruit size and yield efficiency in stone fruit. In this study, the influence of branched secondary alcohol ethoxylate (Tergitol TMN-6) and/or ammonium thiosulfate (ATS), Crocker Fish Oil</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span>(FO), and a mixture of calcium polysulfides and thiosulfate (lime sulfur) on fruit set and quality attributes in peaches (</span><i><span>Prunus</span></i><span> <i>persica</i></span><span>) </span><span>and nectarines (</span><i><span>P</span></i><span><span>. </span><i><span>persica</span></i></span><span> var. </span><i><span>nectarina</span></i><span>) were studied. All blossom thinners reduced fruit set in “Summer Lady” peach in both 2016 and 2017. Fruit sets in “Summer Lady” peach trees </span><span>with a double application of </span><span><span>Tergitol TMN-6 at the rate of 7.5 or 10 mL</span><span></span> <span>·</span><span></span><span></span><span></span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span>, each rate applied at 40% and 80% bloom, were lower than those receiving the same rates of this chemical only at 80% bloom, but they were similar to those receiving a single Tergitol TMN-6 spray at 15 mL</span><span>·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span> at 80% or 100% bloom. “Summer Lady” peach trees receiving FO at 20 mL</span><span>·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span> plus lime sulfur at 25 mL</span><span style="color:#4F4F4F;">·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span>, once at 40% bloom and again at 80% bloom had lower fruit set as compared to Untreated Control but the fruit set was higher than those with any Tergitol-TMN-6 spray. Tergitol TMN-6 at 12 m</span><span style="color:#4F4F4F;">·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span> at 100% bloom or 10 mL</span><span style="color:#4F4F4F;">·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span> all stages of bloom reduced fruit set in “Zee Lady</span></span><span>”</span><span> and </span><span>“</span><span><span>Snow Giant” peaches. In “Snow Giant” peach, trees receiving Tergitol TMN-6 at all concentrations and blossom stages had significantly higher fruit weight as compared to the trees of Un-treated Control.</span><span> </span><span>Applications of Tergitol TMN-6 at all concentrations at 80% bloom reduced fruit set in “Red Globe” and “Elberta” peaches in Utah. ATS and FO slightly reduced fruit set in peaches but they were less effective than Tergitol TMN-6 in all cases. Overall, it is concluded that efficacy of blossom thinners depends on the rate of thinners, temperature, cultivar and stage of bloom development.</span></span>展开更多
基金supported by the Central Fund for Promoting Innovative Technology Development, China (236Z6801G)the Hebei Provincial Modern Seed Industry Technology Innovation Team-Dried Fruits, China (21326304D)the Construction of Innovative Teams for the Modern Agricultural Industry System in Hebei Province, China (HBCT2023120210, HBCT2024190203)。
文摘tone fruits, also known as drupes, have evolved an extremely hard wood-like shell called a stone to protect the seeds. Recently, the market value of stoneless cultivars has risen dramatically, which highlights the needto cultivate stoneless fruit. Therefore, determining the underlying mechanism of fruit stone development isurgently needed. By employing the stone-containing jujube cultivar 'Youhe' and two stoneless Chinese jujube cultivars, 'Wuhefeng' and 'Daguowuhe', we comprehensively studied the mechanism of fruit stone development in jujube. Anatomical analysis and lignin staining revealed that the stone cultivar 'Youhe' jujube exhibited much greater lignin accumulation in the endocarp than the two stoneless cultivars. Lignin accumulation may be the key factor in fruit stone formation. By analyzing the transcriptome data and identifying differentially expressed genes(DEGs), 49 overlapping DEGs were identified in the comparisons of 'Youhe' jujube vs. 'Wuhefeng' jujube and 'Youhe' jujube vs. 'Daguowuhe' jujube. ZjF6H1-3 and ZjPOD, which are involved in lignin synthesis, were identified among these DEGs. The overexpression and silencing of ZjF6H1-3 and ZjPOD in wild jujube seedlings further confirmed their roles in lignin synthesis. In addition, two bHLH transcription factors were included in the 49 overlapping DEGs, and bHLH transcription factor binding motifs were found in the promoters of ZjF6H1-3 and ZjPOD, indicating that bHLH transcription factors are also involved in lignin synthesis and stone formation in Chinese jujube. This study provides new insights into the molecular networks underlying fruit stone formation and can serve as an important reference for the molecular design and breeding of stoneless fruit cultivars of jujube and fruit trees.
文摘The effects of different treatments on cross compatibility in distant hybridization werestudied in 69 cross combinations among 9 cultivars of stone fruits. The results showedthat the setting percentage in balloon stage was obviously higher than that in earlyblooming stage. The differences of fruit set between positive and reverse crosses weresignificant. Female parent affected cross compatibility more remarkably than male parentin distant hybridization. The appropriate electrostatic field, He-Ne laser combinedtreatment with 60Co γ-ray and He-Ne laser could improve the pollen germination rate invitro effectively, the fruit set percentage was higher than control, while 60Co γ-raytreatment reduced the pollen germination rate in vitro, and the fruit set percentageobtained by distant hybridization was lower than control.
文摘<span>Total crop load has an inverse relationship with fruit size, while larger fruit size is often demanded by the peach and nectarine markets. Hand-thinning is extremely expensive, and thus, crop load adjustment, using blossom thinners is a crucial practice to ensure production of commercially acceptable fruit size and yield efficiency in stone fruit. In this study, the influence of branched secondary alcohol ethoxylate (Tergitol TMN-6) and/or ammonium thiosulfate (ATS), Crocker Fish Oil</span><span style="font-family:;" "=""> </span><span style="font-family:;" "=""><span>(FO), and a mixture of calcium polysulfides and thiosulfate (lime sulfur) on fruit set and quality attributes in peaches (</span><i><span>Prunus</span></i><span> <i>persica</i></span><span>) </span><span>and nectarines (</span><i><span>P</span></i><span><span>. </span><i><span>persica</span></i></span><span> var. </span><i><span>nectarina</span></i><span>) were studied. All blossom thinners reduced fruit set in “Summer Lady” peach in both 2016 and 2017. Fruit sets in “Summer Lady” peach trees </span><span>with a double application of </span><span><span>Tergitol TMN-6 at the rate of 7.5 or 10 mL</span><span></span> <span>·</span><span></span><span></span><span></span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span>, each rate applied at 40% and 80% bloom, were lower than those receiving the same rates of this chemical only at 80% bloom, but they were similar to those receiving a single Tergitol TMN-6 spray at 15 mL</span><span>·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span> at 80% or 100% bloom. “Summer Lady” peach trees receiving FO at 20 mL</span><span>·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span> plus lime sulfur at 25 mL</span><span style="color:#4F4F4F;">·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span>, once at 40% bloom and again at 80% bloom had lower fruit set as compared to Untreated Control but the fruit set was higher than those with any Tergitol-TMN-6 spray. Tergitol TMN-6 at 12 m</span><span style="color:#4F4F4F;">·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span><span> at 100% bloom or 10 mL</span><span style="color:#4F4F4F;">·</span><span>L</span><span style="color:#4F4F4F;">¯</span><span></span></span><sup><span>1</span></sup><span> all stages of bloom reduced fruit set in “Zee Lady</span></span><span>”</span><span> and </span><span>“</span><span><span>Snow Giant” peaches. In “Snow Giant” peach, trees receiving Tergitol TMN-6 at all concentrations and blossom stages had significantly higher fruit weight as compared to the trees of Un-treated Control.</span><span> </span><span>Applications of Tergitol TMN-6 at all concentrations at 80% bloom reduced fruit set in “Red Globe” and “Elberta” peaches in Utah. ATS and FO slightly reduced fruit set in peaches but they were less effective than Tergitol TMN-6 in all cases. Overall, it is concluded that efficacy of blossom thinners depends on the rate of thinners, temperature, cultivar and stage of bloom development.</span></span>
