Aim:To analyze the effective methods of antibiotic ointment to control bacterial growth,and to provide references for the research work of related personnel.Method:In this experiment,membrane filtration method combine...Aim:To analyze the effective methods of antibiotic ointment to control bacterial growth,and to provide references for the research work of related personnel.Method:In this experiment,membrane filtration method combined with centrifugal precipitation method was used to complete the inspection of antibiotic ointment,and the final result was analyzed.Results:The application of membrane filtration method combined with centrifugal precipitation method to complete the test of antibiotic ointment has a significant effect.The test solution is milky white.The possibility if it can exert the effect of eliminating antibacterial components,so that Pseudomonas aeruginosa and Staphylococcus caureus can show an affecting state in the positive test tube,and colonies can be found after observation.Conclusion:The application of membrane filtration method combined with centrifugal precipitation method to complete the inspection of antibiotic ointment can achieve satisfactory results.The method is simple to operate and has high feasibility.It is worthy of further promotion and application.展开更多
目的探索药品控制菌检查中适合的菌种鉴定方法。方法采用VITEK 2 Compact全自动微生物生化鉴定系统和3500 Micro Seq全自动微生物基因分型鉴定系统,对控制菌检查中常见的32株菌进行生化反应鉴定和16S r DNA测序鉴定,并对鉴定结果为沙门...目的探索药品控制菌检查中适合的菌种鉴定方法。方法采用VITEK 2 Compact全自动微生物生化鉴定系统和3500 Micro Seq全自动微生物基因分型鉴定系统,对控制菌检查中常见的32株菌进行生化反应鉴定和16S r DNA测序鉴定,并对鉴定结果为沙门菌的菌株进行血清分型。结果 2种方法鉴定结果基本一致,部分菌株生化反应鉴定只能至属,而16S r DNA测序可以鉴定至种甚至亚种水平,沙门菌的分型需采用血清学鉴定。结论药品控制菌检查可结合16S r DNA测序和生化鉴定,选择不同控制菌适合的鉴定方法。展开更多
文摘Aim:To analyze the effective methods of antibiotic ointment to control bacterial growth,and to provide references for the research work of related personnel.Method:In this experiment,membrane filtration method combined with centrifugal precipitation method was used to complete the inspection of antibiotic ointment,and the final result was analyzed.Results:The application of membrane filtration method combined with centrifugal precipitation method to complete the test of antibiotic ointment has a significant effect.The test solution is milky white.The possibility if it can exert the effect of eliminating antibacterial components,so that Pseudomonas aeruginosa and Staphylococcus caureus can show an affecting state in the positive test tube,and colonies can be found after observation.Conclusion:The application of membrane filtration method combined with centrifugal precipitation method to complete the inspection of antibiotic ointment can achieve satisfactory results.The method is simple to operate and has high feasibility.It is worthy of further promotion and application.
文摘目的探索药品控制菌检查中适合的菌种鉴定方法。方法采用VITEK 2 Compact全自动微生物生化鉴定系统和3500 Micro Seq全自动微生物基因分型鉴定系统,对控制菌检查中常见的32株菌进行生化反应鉴定和16S r DNA测序鉴定,并对鉴定结果为沙门菌的菌株进行血清分型。结果 2种方法鉴定结果基本一致,部分菌株生化反应鉴定只能至属,而16S r DNA测序可以鉴定至种甚至亚种水平,沙门菌的分型需采用血清学鉴定。结论药品控制菌检查可结合16S r DNA测序和生化鉴定,选择不同控制菌适合的鉴定方法。
