摘要
以玉簪"法兰西"组培苗为材料,通过对其内生菌的分离鉴定和药敏试验,探讨抗生素药剂处理时间、浓度、种类对玉簪试管苗内生菌污染的控制.结果表明:玉簪试管苗污染的菌种为克氏库克菌,通过药敏试验可知,该菌对链霉素和林可霉素产生不同程度的耐药性,而庆大霉素、阿莫西林、四环素等13种抗菌药物对克氏库克菌的抑制效果较好.在添加抗生素降低玉簪试管苗污染试验中,将污染的试管苗分别浸泡在100 mg/L青霉素和头孢唑啉溶液中30 min,抗菌率分别为24.03%、26.65%.培养基中添加400 mg/L青霉素,抗菌率可达74.06%,芽增殖系数为3.46,平均新增叶数为3.75片,平均增高1.35 cm,苗长势健壮;添加800 mg/L头孢唑啉,抗菌率最高为74.48%,芽增殖系数为4.25,平均新增叶数4.18片,平均增高0.81 cm,苗长势良好,未作处理的对照组抗菌率为0.
With the tissue culture seedlings of Hosta ‘Franc＇as material, the authors isolate and identify the endophytic bacteria, and tests the drug sensitivity of it, and study on how the treatment time, concentration, kind control bacteria contaminnation of host seedlings.The results showed that the bacterium of pollution of tissue culture seedling named Klebsiella bacteria strains for Micrococcus Kristinae.Drug sensitivity test showed that, the bacterium can endure the streptomycin and Lincolnensis can mycin on varying degrees, besides,Gentamycin, Amoxicillin, Tetracycline and other 13 kinds of antibiotics can inhibit the Micrococcus Kristinae bacteria effectively. The testing of reducing the tissue culture seedling pollution by Antibiotics showed that, dipping the contaminated plantlets in 100 mg/L Benzylpenicillin and Cefazolin for 30 min, which antibacterial rate was 24.03% and 26.65% respectively. With the addition of 400 mg/L Benzylpenicillin to the culture medium, the antibacterial rate was 74.06%. Growth coefficient was 3.46 average number, and the increased of new leaf was 3.75, and increased 1.35 cm on average, and the seedlings are growing better. With the addition of 800 ma/L Cefazolin to the culture medium, the antibacterial rete was 74.48%, and growth coefficient was 4.25, and the increased of new leaf was 4.18 on average,and increased 0.81 cm, which seedlings are growing well. Finally the antibacterial rate of the control group was 0.
出处
《农业科学研究》
2016年第4期64-68,共5页
Journal of Agricultural Sciences
基金
宁夏科技支撑计划资助项目(2014ZZNO9)
"宁夏设施园艺提升发展研究与示范"子课题--小盆花品种资源收集与种苗快繁技术研究与示范
关键词
玉簪
试管苗
内生菌
抗生素
污染控制
hosta
test-tube plantlet
endophytic bacteria
antibiotics
pollution control
