Objective: To isolate endophytic fungi from Elaeocarpus sylvestris(E. sylvestris) and to isolate antioxidant compounds from a potential source fungus.Methods: Endophytic fungi were isolated from fresh leaves and stems...Objective: To isolate endophytic fungi from Elaeocarpus sylvestris(E. sylvestris) and to isolate antioxidant compounds from a potential source fungus.Methods: Endophytic fungi were isolated from fresh leaves and stems of E. sylvestris and identified based on DNA analysis. 1,1-Diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity was used to evaluate the antioxidant activity of the fungi. The potential antioxidant fungus was further studied to isolate antioxidant compounds. The isolated compounds were identified by melting point analysis, optical rotation, spectral analysis using a UV spectrophotometer, high resolution fast atom bombardment mass spectrometry, X-ray crystallography analysis,~1H nuclear magnetic resonance analysis and ^(13)C nuclear magnetic resonance analysis. The isolated compounds were evaluated with DPPH radical scavenging, reducing power, and b-carotene bleaching assays.Results: Seven endophytic fungi were successfully isolated from E. sylvestris and identified as Pestalotiopsis sp. EST 01, Pestalotiopsis sp. EST 02, Diaporthales sp. EST03, Meyerozyma sp. EST 04, Diaporthales sp. EST 05, Pestalotiopsis sp. ESL 01, and Pseudocercospora sp. ESL 02. Of the seven fungi, Pseudocercospora sp. ESL 02 had the highest antioxidant activity [IC50=(30.54 ± 0.88) mg/mL]. From that fungus, two compounds identified as terreic acid(1) and 6-methylsalicylic acid(2) were isolated with an IC50 of DPPH radical scavenging activity of(0.22 ± 0.02) mmol/L and(3.87 ± 0.27)mmol/L, respectively. The compounds also had good activities from the reducing power and b-carotene bleaching assays.Conclusions: The Pseudocercospora sp. ESL 02 fungus isolated from E. sylvestris looks promising as a novel source of terreic acid.展开更多
目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期...目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期临床个体化干预提供参考。方法选取2020年3月—2025年3月河北省胸科医院收治的TRALI患者249例为研究对象,根据输血后30 d内预后情况,分别纳入预后良好组(178例)、预后不良组(71例)。比较2组一般临床资料及血清SP-D、MUC1、A20水平,Logistic回归分析血清SP-D、MUC1、A20水平对TRALI患者预后不良的影响因素,受试者工作特征(receiver operating characteristic,ROC)曲线分析血清SP-D、MUC1、A20水平单独及联合检测对预后不良的预测效能,并采用一致性分析进行外部验证。结果2组输血次数、发血至输血时间、过敏史、急性生理与慢性健康评分系统Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)评分比较,差异有统计学意义(P<0.05);预后不良组血清SP-D、MUC1、A20水平分别为(89.54±21.36)g/L、(22.97±5.14)kU/L、(14.53±1.96)mg/L,明显高于预后良好组的(78.61±18.05)g/L、(16.28±4.32)kU/L、(12.67±1.84)mg/L,差异有统计学意义(P<0.05);Logistic回归分析显示,输血次数、发血至输血时间、过敏史均是TRALI患者预后不良的影响因素(P<0.05),APACHEⅡ评分及血清SP-D、MUC1、A20均与TRALI患者预后不良显著相关(P<0.05);ROC分析显示,血清SP-D、MUC1、A20联合预测预后不良的曲线下面积(area under the curve,AUC)为0.904(95%CI:0.860~0.938),优于各指标单独预测价值(Z=2.507、3.016、3.042,均P<0.05),且外部验证显示,联合预测预后不良与临床实际的符合率为95.00%,Kappa值为0.870(95%CI:0.617~0.982)差异有统计学意义(P<0.05)。结论血清SP-D、MUC1、A20均是TRALI患者预后不良的独立影响因素,联合检测对预后不良具有较高预测价值,可作为TRALI患者预后的潜在预测因子,并可指导临床工作。展开更多
Mildew resistance locus O(MLO)proteins are extensively found in various plant species and are essential for multiple biological functions.The characterization and analysis of MLO genes have been conducted across numer...Mildew resistance locus O(MLO)proteins are extensively found in various plant species and are essential for multiple biological functions.The characterization and analysis of MLO genes have been conducted across numerous species.However,the functions and features of MLO genes inside sugar beet remain poorly understood.In the present research,we conducted a comprehensive analysis of the structural features of MLO genes,physicochemical characteristics of proteins,evolutionary connections,and expression profiles in sugar beet.A total of 13 BvMLO genes containing MLO structural domains were detected and renamed based on their locations on chromosomes within the sugar beet genome.According to the classification of AtMLO genes,the evolutionary analysis revealed that these 13 BvMLO genes were classified into three subgroups and unevenly located across four chromosomes.Synteny and collinearity analysis confirmed that gene clusters occurred during the evolution of the BvMLO gene family.Examination of cis-regulatory elements revealed specific stress-induced and hormone-associated components within the regulatory regions of BvMLOs.We also found that the expression levels of BvMLO2 and BvMLO7 cloned from sugar beet plants inoculated by Erysiphe betae(Vanha)were significantly regulated by Cercospora beticola Sacc(C.beticola),which indicated that they might both participate in some disease resistance processes.Moreover,quantitative real-time PCR(qRT-PCR)results confirmed that BvMLO2 and BvMLO7 were involved in plant resistance to various biotic and abiotic stress factors.Overall,this research provides a fundamental basis for upcoming studies on the functions and control mechanisms of BvMLO genes within sugar beet.These research findings help advance the progress of disease-resistant breeding in sugar beet and enhance the effectiveness of its resistance breeding.展开更多
Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,red...Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,reducing hydrophobicity and decreasing tensile strength.Additionally,pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film.AS3–8 consortia exhibited a maximum weight loss of 8.0%±0.5%after 45 days of incubation.While Bacillus sp.AS3 and Sphingobacterium sp.AS8 demonstrated LDPE weight loss of 5.03%±1.6%and 1.6%±0.5%,respectively.The structure of LDPE was altered after incubation with the bacterial strains,resulting in a reduction in the intensity of functional groups,including C=O,C=C,N–H,and C–N.The carbonyl index(CI)of LDPE also decreased by 7.17%after the consortia AS3–8 degradation.Consortia AS3–8 significantly impacted the physical properties of LDPE by reducing the water contact angle(WCA),decreasing to 64.21°±3.69°,and tensile strength(TS),decreasing to 17.97±0.3 MPa.Moreover,the esterase activity was measured through 45 days of incubation.SDS-PAGE analysis of the AS3–8 consortia revealed bands at 35,48,and 70 kDa molecular weights,similar to known enzymes like laccase and esterase.Furthermore,SEM observations showed rough,cracked surfaces on pretreated LDPE,with biofilms present after incubation with the bacterial strains.GC–MS analysis revealed that AS3–8 consortia produced depolymerized chemicals,including alkanes,aldehydes,and esters.The LDPE biodegradation pathway was elucidated.This study addresses critical knowledge gaps in improving plastic degradation efficiency.展开更多
文摘Objective: To isolate endophytic fungi from Elaeocarpus sylvestris(E. sylvestris) and to isolate antioxidant compounds from a potential source fungus.Methods: Endophytic fungi were isolated from fresh leaves and stems of E. sylvestris and identified based on DNA analysis. 1,1-Diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity was used to evaluate the antioxidant activity of the fungi. The potential antioxidant fungus was further studied to isolate antioxidant compounds. The isolated compounds were identified by melting point analysis, optical rotation, spectral analysis using a UV spectrophotometer, high resolution fast atom bombardment mass spectrometry, X-ray crystallography analysis,~1H nuclear magnetic resonance analysis and ^(13)C nuclear magnetic resonance analysis. The isolated compounds were evaluated with DPPH radical scavenging, reducing power, and b-carotene bleaching assays.Results: Seven endophytic fungi were successfully isolated from E. sylvestris and identified as Pestalotiopsis sp. EST 01, Pestalotiopsis sp. EST 02, Diaporthales sp. EST03, Meyerozyma sp. EST 04, Diaporthales sp. EST 05, Pestalotiopsis sp. ESL 01, and Pseudocercospora sp. ESL 02. Of the seven fungi, Pseudocercospora sp. ESL 02 had the highest antioxidant activity [IC50=(30.54 ± 0.88) mg/mL]. From that fungus, two compounds identified as terreic acid(1) and 6-methylsalicylic acid(2) were isolated with an IC50 of DPPH radical scavenging activity of(0.22 ± 0.02) mmol/L and(3.87 ± 0.27)mmol/L, respectively. The compounds also had good activities from the reducing power and b-carotene bleaching assays.Conclusions: The Pseudocercospora sp. ESL 02 fungus isolated from E. sylvestris looks promising as a novel source of terreic acid.
文摘目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期临床个体化干预提供参考。方法选取2020年3月—2025年3月河北省胸科医院收治的TRALI患者249例为研究对象,根据输血后30 d内预后情况,分别纳入预后良好组(178例)、预后不良组(71例)。比较2组一般临床资料及血清SP-D、MUC1、A20水平,Logistic回归分析血清SP-D、MUC1、A20水平对TRALI患者预后不良的影响因素,受试者工作特征(receiver operating characteristic,ROC)曲线分析血清SP-D、MUC1、A20水平单独及联合检测对预后不良的预测效能,并采用一致性分析进行外部验证。结果2组输血次数、发血至输血时间、过敏史、急性生理与慢性健康评分系统Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)评分比较,差异有统计学意义(P<0.05);预后不良组血清SP-D、MUC1、A20水平分别为(89.54±21.36)g/L、(22.97±5.14)kU/L、(14.53±1.96)mg/L,明显高于预后良好组的(78.61±18.05)g/L、(16.28±4.32)kU/L、(12.67±1.84)mg/L,差异有统计学意义(P<0.05);Logistic回归分析显示,输血次数、发血至输血时间、过敏史均是TRALI患者预后不良的影响因素(P<0.05),APACHEⅡ评分及血清SP-D、MUC1、A20均与TRALI患者预后不良显著相关(P<0.05);ROC分析显示,血清SP-D、MUC1、A20联合预测预后不良的曲线下面积(area under the curve,AUC)为0.904(95%CI:0.860~0.938),优于各指标单独预测价值(Z=2.507、3.016、3.042,均P<0.05),且外部验证显示,联合预测预后不良与临床实际的符合率为95.00%,Kappa值为0.870(95%CI:0.617~0.982)差异有统计学意义(P<0.05)。结论血清SP-D、MUC1、A20均是TRALI患者预后不良的独立影响因素,联合检测对预后不良具有较高预测价值,可作为TRALI患者预后的潜在预测因子,并可指导临床工作。
文摘Mildew resistance locus O(MLO)proteins are extensively found in various plant species and are essential for multiple biological functions.The characterization and analysis of MLO genes have been conducted across numerous species.However,the functions and features of MLO genes inside sugar beet remain poorly understood.In the present research,we conducted a comprehensive analysis of the structural features of MLO genes,physicochemical characteristics of proteins,evolutionary connections,and expression profiles in sugar beet.A total of 13 BvMLO genes containing MLO structural domains were detected and renamed based on their locations on chromosomes within the sugar beet genome.According to the classification of AtMLO genes,the evolutionary analysis revealed that these 13 BvMLO genes were classified into three subgroups and unevenly located across four chromosomes.Synteny and collinearity analysis confirmed that gene clusters occurred during the evolution of the BvMLO gene family.Examination of cis-regulatory elements revealed specific stress-induced and hormone-associated components within the regulatory regions of BvMLOs.We also found that the expression levels of BvMLO2 and BvMLO7 cloned from sugar beet plants inoculated by Erysiphe betae(Vanha)were significantly regulated by Cercospora beticola Sacc(C.beticola),which indicated that they might both participate in some disease resistance processes.Moreover,quantitative real-time PCR(qRT-PCR)results confirmed that BvMLO2 and BvMLO7 were involved in plant resistance to various biotic and abiotic stress factors.Overall,this research provides a fundamental basis for upcoming studies on the functions and control mechanisms of BvMLO genes within sugar beet.These research findings help advance the progress of disease-resistant breeding in sugar beet and enhance the effectiveness of its resistance breeding.
文摘Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,reducing hydrophobicity and decreasing tensile strength.Additionally,pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film.AS3–8 consortia exhibited a maximum weight loss of 8.0%±0.5%after 45 days of incubation.While Bacillus sp.AS3 and Sphingobacterium sp.AS8 demonstrated LDPE weight loss of 5.03%±1.6%and 1.6%±0.5%,respectively.The structure of LDPE was altered after incubation with the bacterial strains,resulting in a reduction in the intensity of functional groups,including C=O,C=C,N–H,and C–N.The carbonyl index(CI)of LDPE also decreased by 7.17%after the consortia AS3–8 degradation.Consortia AS3–8 significantly impacted the physical properties of LDPE by reducing the water contact angle(WCA),decreasing to 64.21°±3.69°,and tensile strength(TS),decreasing to 17.97±0.3 MPa.Moreover,the esterase activity was measured through 45 days of incubation.SDS-PAGE analysis of the AS3–8 consortia revealed bands at 35,48,and 70 kDa molecular weights,similar to known enzymes like laccase and esterase.Furthermore,SEM observations showed rough,cracked surfaces on pretreated LDPE,with biofilms present after incubation with the bacterial strains.GC–MS analysis revealed that AS3–8 consortia produced depolymerized chemicals,including alkanes,aldehydes,and esters.The LDPE biodegradation pathway was elucidated.This study addresses critical knowledge gaps in improving plastic degradation efficiency.
