Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of ...Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of Cangfu Daotan pills were searched in the database of traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP).GeneCards database and OMIM database were used to search for disease targets of PCOS;the intersection of the target of Cangfu Daotan pill and the disease target of PCOS was drawn by using R to obtain the intersection gene;protein interaction network was constructed through String online database;Cytoscape(3.2.1)was used to construct a‘major component-disease-target’network diagram and analyze the important nodes therein;KEGG and GO enrichment analyses were carried out on the obtained intersection genes through org data packets in R.Results:Network pharmacology analysis showed that 151 active ingredients involved in pathways were screened out,140 of which acted on PCOS,and 105 potential targets were used to exert therapeutic effects.After enrichment,1900 GO biological signals and 143 KEGG signals were obtained,and the first 14 drug targets were screened out.Conclusion:Cangfu Daotan pill may mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation and other signals through regulating key targets of JUN,MAPK3,HSP90AA1,MAPK1,ESR1,NR3C1,APP,MAPK14,MAPK8,VEGFA,EGFR,RB1,AR,IL6,mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation,etc.,and regulate biological processes such as drug reaction,nutrition and lipid metabolism,active oxygen,sugar metabolism,steroid hormone,etc.to achieve the effect of treating polycystic ovary syndrome.展开更多
目的分析温针灸联合苍附导痰汤加减治疗痰湿型多囊卵巢综合征(PCOS)的临床疗效及对胰岛素抵抗、排卵功能的影响。方法将本院2023年3月至2025年3月收治的60例痰湿型PCOS患者按随机抽签法分为两组,对照组30例接受苍附导痰汤加减治疗,实验...目的分析温针灸联合苍附导痰汤加减治疗痰湿型多囊卵巢综合征(PCOS)的临床疗效及对胰岛素抵抗、排卵功能的影响。方法将本院2023年3月至2025年3月收治的60例痰湿型PCOS患者按随机抽签法分为两组,对照组30例接受苍附导痰汤加减治疗,实验组30例在对照组基础上联合温针灸治疗,比较两组临床疗效、胰岛素抵抗指标、性激素水平、炎症水平、排卵功能及妊娠情况差异。结果实验组90.00%(27/30)总有效率高于对照组66.67%(20/30)(P<0.05);治疗后,实验组空腹胰岛素(FINS)、餐后2 h胰岛素(2 h INS)、稳态模型评估IR指数(HOMA-IR)、抗缪勒管激素(AMH)、促黄体生成素(LH)、白细胞介素(IL)-6、IL-18、IL-1β、肿瘤坏死因子(TNF)-α低于对照组,雌二醇(E2)、卵泡刺激素(FSH)高于对照组(P<0.05);实验组排卵率83.33%(25/30)、妊娠率73.33%(22/30)高于对照组53.33%(16/30)、43.33%(13/30)(P<0.05)。结论温针灸联合苍附导痰汤加减治疗痰湿型PCOS的临床效果良好,可改善患者胰岛素抵抗,调节性激素及炎症水平,提升排卵功能及妊娠成功率,值得推广。展开更多
基金Shanghai“Xinglin New Star”project[ZY(2018-2020)-RCPY-3012]Clinical study on improving the endometrial receptivity of patients with polycystic ovary syndrome by the method of invigorating the kidney,removing phlegm and promoting blood circulation(Project within budget of Shanghai university of traditional Chinese medicine)+1 种基金The construction project of Shanghai famous old traditional Chinese medicine experience research studio(No.SHGZS-2017026)Shanghai university of traditional Chinese medicine affiliated Shuguang hospital Siming fund(In-hospital project)
文摘Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of Cangfu Daotan pills were searched in the database of traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP).GeneCards database and OMIM database were used to search for disease targets of PCOS;the intersection of the target of Cangfu Daotan pill and the disease target of PCOS was drawn by using R to obtain the intersection gene;protein interaction network was constructed through String online database;Cytoscape(3.2.1)was used to construct a‘major component-disease-target’network diagram and analyze the important nodes therein;KEGG and GO enrichment analyses were carried out on the obtained intersection genes through org data packets in R.Results:Network pharmacology analysis showed that 151 active ingredients involved in pathways were screened out,140 of which acted on PCOS,and 105 potential targets were used to exert therapeutic effects.After enrichment,1900 GO biological signals and 143 KEGG signals were obtained,and the first 14 drug targets were screened out.Conclusion:Cangfu Daotan pill may mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation and other signals through regulating key targets of JUN,MAPK3,HSP90AA1,MAPK1,ESR1,NR3C1,APP,MAPK14,MAPK8,VEGFA,EGFR,RB1,AR,IL6,mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation,etc.,and regulate biological processes such as drug reaction,nutrition and lipid metabolism,active oxygen,sugar metabolism,steroid hormone,etc.to achieve the effect of treating polycystic ovary syndrome.
文摘目的分析温针灸联合苍附导痰汤加减治疗痰湿型多囊卵巢综合征(PCOS)的临床疗效及对胰岛素抵抗、排卵功能的影响。方法将本院2023年3月至2025年3月收治的60例痰湿型PCOS患者按随机抽签法分为两组,对照组30例接受苍附导痰汤加减治疗,实验组30例在对照组基础上联合温针灸治疗,比较两组临床疗效、胰岛素抵抗指标、性激素水平、炎症水平、排卵功能及妊娠情况差异。结果实验组90.00%(27/30)总有效率高于对照组66.67%(20/30)(P<0.05);治疗后,实验组空腹胰岛素(FINS)、餐后2 h胰岛素(2 h INS)、稳态模型评估IR指数(HOMA-IR)、抗缪勒管激素(AMH)、促黄体生成素(LH)、白细胞介素(IL)-6、IL-18、IL-1β、肿瘤坏死因子(TNF)-α低于对照组,雌二醇(E2)、卵泡刺激素(FSH)高于对照组(P<0.05);实验组排卵率83.33%(25/30)、妊娠率73.33%(22/30)高于对照组53.33%(16/30)、43.33%(13/30)(P<0.05)。结论温针灸联合苍附导痰汤加减治疗痰湿型PCOS的临床效果良好,可改善患者胰岛素抵抗,调节性激素及炎症水平,提升排卵功能及妊娠成功率,值得推广。
