dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV ...dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of展开更多
The gut is not only used by insects as an organ for the digestion of food and absorption of nutrients but also as an important barrier against the invasion and proliferation of pathogenic microorganisms.Bombyx mori cy...The gut is not only used by insects as an organ for the digestion of food and absorption of nutrients but also as an important barrier against the invasion and proliferation of pathogenic microorganisms.Bombyx mori cytoplasmic polyhedrosis virus(BmCPV),an insect-specific virus,predominantly colonizes the midgut epithelial cells of the silkworm,thereby jeopardizing its normal growth.However,there is limited knowledge of the cellular immune responses to viral infection and whether the infection is promoted or inhibited by different types of cells in the silkworm midgut.In this study,we used single-nucleus RNA sequencing to identify representative enteroendocrine cells,enterocytes,and muscle cell types in the silkworm midgut.In addition,by analyzing the transcriptional profiles of various subpopulations in the infected and uninfected groups,we found that BmCPV infection suppresses the response of the antiviral pathways and induces the expression of BmHSP70,which plays a role in promoting BmCPV replication.However,certain immune genes in the midgut of the silkworm,such as BmLebocin3,were induced upon viral infection,and downregulation of BmLEB3 using RNA interference promoted BmCPV replication in the midgut of B.mori.These results suggest that viral immune evasion and active host resistance coexist in BmCPV-infected silkworms.We reveal the richness of cellular diversity in the midgut of B.mori larvae by single-nucleus RNA sequencing analysis and provide new insights into the complex interactions between the host and the virus at the single-cell level.展开更多
文摘dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of
基金supported by the National Natural Science Foundation of China(No.32372951)。
文摘The gut is not only used by insects as an organ for the digestion of food and absorption of nutrients but also as an important barrier against the invasion and proliferation of pathogenic microorganisms.Bombyx mori cytoplasmic polyhedrosis virus(BmCPV),an insect-specific virus,predominantly colonizes the midgut epithelial cells of the silkworm,thereby jeopardizing its normal growth.However,there is limited knowledge of the cellular immune responses to viral infection and whether the infection is promoted or inhibited by different types of cells in the silkworm midgut.In this study,we used single-nucleus RNA sequencing to identify representative enteroendocrine cells,enterocytes,and muscle cell types in the silkworm midgut.In addition,by analyzing the transcriptional profiles of various subpopulations in the infected and uninfected groups,we found that BmCPV infection suppresses the response of the antiviral pathways and induces the expression of BmHSP70,which plays a role in promoting BmCPV replication.However,certain immune genes in the midgut of the silkworm,such as BmLebocin3,were induced upon viral infection,and downregulation of BmLEB3 using RNA interference promoted BmCPV replication in the midgut of B.mori.These results suggest that viral immune evasion and active host resistance coexist in BmCPV-infected silkworms.We reveal the richness of cellular diversity in the midgut of B.mori larvae by single-nucleus RNA sequencing analysis and provide new insights into the complex interactions between the host and the virus at the single-cell level.
