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从云南蚕区分离BmCPV病毒株的全基因组克隆与系统发育分析 被引量：1

Whole-genome Cloning and Phylogenetic Analysis of a New BmCPV Strain Isolated from Sericultural Region of Yunnan
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摘要 家蚕质型多角体病毒（BmCPV）是家蚕中肠型脓病的病原物。从云南蚕区的病蚕样品中分离到一株BmCPV病毒分离株,提取纯化该病毒粒子的总RNA并合成cDNA,克隆病毒全基因组序列,研究该病毒分离株的基因组结构与系统进化。克隆得到该病毒10条dsRNA序列（S1~S10）并提交至NCBI数据库中。序列分析表明该病毒基因组序列全长24810bp,S1~S10片段序列都具有完整的ORF。BLAST比对发现克隆的该病毒基因组各片段编码区序列与已报道的1型BmCPV片段的相似度达88%以上,氨基酸序列相似度达90%以上;10条dsRNA基因组片段末端均有保守帽子结构5＇-AGUAA……GUUAGCC-3＇。基于S2片段RdRp基因的系统进化分析发现,该病毒与其他3个1型BmCPV分离株BmCPV1-Suzhou、BmCPV1-China、BmCPV1-Ⅰ聚为一支,基于S10片段polh基因的系统进化分析也表明该病毒为1型BmCPV新的分离株系。根据上述研究结果将该病毒定名为BmCPV1-Yunnan。 The pathogen of silkworm （ Bombyx mori） midgut polydhedrosis is Bombyx mori cytoplasmic polyhedosis vi- rus （BmCPV）. We isolated a new BmCPV straJn from diseased silkworm Jn Yunnan sericultural region. After total RNA was extracted and purified, cDNA was synthesized to clone the whole genomic sequence for study of genomic structure and phylogenetic evolution of this new viral strain. Totally 10 dsRNA sequences namely S1 to S10 were cloned and uploaded to NCBI database. Sequence analysis indicated that the full-length of genome was 24 810 bp, and all seg- ments possessed a complete open reading frame （ORF）. BLAST alignment showed that the nucleotide sequence identity of coding region between each genomic segment and reported BmCPV 1 isolates was over 88%, and the identity of amino acid sequences was over 90%. All genomic segments possessed the conserved terminal sequences AGUAA and GUUAGCC at 5＇ and 3＇ ends respectively. Phylogenetic analysis based on RdRp gene of S2 segment showed that this viral strain and other three BmCPV 1 isolates （ BmCPV1-Suzhou, BmCPV1-China and BmCPV1-I） formed a sepa-rate clade. Phylogenetic analysis based on polh gene of S10 segment also verified that the viral strain is a new BmCPV 1 isolate. Based on the above results, the new viral strain isolated from Yunnan sericultural region was named as BmCPV1-Yunnan.
作者 张永红 唐芬芬 邵榆岚 朱峰 白兴荣
机构地区 云南省农业科学院蚕桑蜜蜂研究所
出处 《蚕业科学》 CAS CSCD 北大核心 2015年第6期1036-1043,共8页 ACTA SERICOLOGICA SINICA
基金 云南省现代农业蚕桑产业技术体系项目(No.2013KJTX00-6) 现代农业产业技术体系建设专项(No.CARS-22)
关键词 家蚕质型多角体病毒 分离株 基因组片段 系统进化 RNA聚合酶基因 多角体蛋白基因 Bombyx mori cytoplasmic polyhedosis virus（BmCPV） Isolate Genomic segment Phylogenetic evolution RNA polymerase gene （RdRp） Polyhedrin gene （polh）
分类号 S884.5 [农业科学—特种经济动物饲养] Q785 [生物学—分子生物学]
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参考文献37

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二级参考文献44

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蚕业科学
2015年 第6期

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