The viability and biological activity of Absidia coerulea in compressed or supercritical CO2 and C2H4 were studied. The specific activity of Absidia coerulea in 7.5MPa CO2 and C2H4 at 306K can reach to 23% and 75% r...The viability and biological activity of Absidia coerulea in compressed or supercritical CO2 and C2H4 were studied. The specific activity of Absidia coerulea in 7.5MPa CO2 and C2H4 at 306K can reach to 23% and 75% respectively, leading to the feasibility of using supercritical C2H4 as an alternative to the organic solvent in the hydroxylation of Reichsterin’s substance acetate.展开更多
Two kinds of micro organism, Arthrobacter sp. AX86(1,4 dehdrogenator)and Absidia sp. A28(11α hydroxylator)were used in this experiment.Two different fermentation techniques were performed to accomplish the multiple c...Two kinds of micro organism, Arthrobacter sp. AX86(1,4 dehdrogenator)and Absidia sp. A28(11α hydroxylator)were used in this experiment.Two different fermentation techniques were performed to accomplish the multiple conversional reactions for producing 16β methyl 11α,17α,21 trihydroxy 1,4 pregnadiene 3,20 dione(Ⅲ)from 16β methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate(I):1)To produce product(Ⅲ)by means of a two step fermentation method which were independently performed first by Arthrobacter and next by Absiaia, and 2)the product was obtained by a sequential fermentation system of aforesaid two micro organisms in a single fermentor without isolation of the intermediates from the mixture.Our results showed that in both fermentation systems high yield of product was obtained.However,according to the technical simplicity,shorter duration of fermentation cycle and efficient yield of product,the second method is better than the first one.展开更多
基金support from the NNSFC(No.20076033,20176041)is greatly acknowledged
文摘The viability and biological activity of Absidia coerulea in compressed or supercritical CO2 and C2H4 were studied. The specific activity of Absidia coerulea in 7.5MPa CO2 and C2H4 at 306K can reach to 23% and 75% respectively, leading to the feasibility of using supercritical C2H4 as an alternative to the organic solvent in the hydroxylation of Reichsterin’s substance acetate.
文摘采用固体平板培养和液体发酵筛选产生甲壳素脱乙酰酶的真菌,并且研究了产酶条件.结果显示在42株真菌中有26株有甲壳素脱乙酰酶活性,最终确定了构巢曲霉和蓝色犁头霉两株产酶活性较高的菌株,它们产生的酶活性分别是343U·mL-1和289 U·mL-1.不同的菌株的产酶培养基中的碳源均为含乙酰基的碳源,最适氮源分别为酵母粉和蛋白胨,产酶能力都受Mn2+和Co2+促进,受Cu2+、Fe2+、Fe3+的抑制;构巢曲霉的最适温度、最适pH分别为29 ℃,7.0～7.5,蓝色犁头霉的最适温度、最适pH分别为31 ℃,6.5～7.0,最适发酵时间均为96 h.
文摘Two kinds of micro organism, Arthrobacter sp. AX86(1,4 dehdrogenator)and Absidia sp. A28(11α hydroxylator)were used in this experiment.Two different fermentation techniques were performed to accomplish the multiple conversional reactions for producing 16β methyl 11α,17α,21 trihydroxy 1,4 pregnadiene 3,20 dione(Ⅲ)from 16β methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate(I):1)To produce product(Ⅲ)by means of a two step fermentation method which were independently performed first by Arthrobacter and next by Absiaia, and 2)the product was obtained by a sequential fermentation system of aforesaid two micro organisms in a single fermentor without isolation of the intermediates from the mixture.Our results showed that in both fermentation systems high yield of product was obtained.However,according to the technical simplicity,shorter duration of fermentation cycle and efficient yield of product,the second method is better than the first one.
