Protein kinases play an important role in every aspect of cellular life.In this study,we systemically identified protein kinases from the predicted proteomes of 59 representative fungi from Ascomycota and Basidiomycot...Protein kinases play an important role in every aspect of cellular life.In this study,we systemically identified protein kinases from the predicted proteomes of 59 representative fungi from Ascomycota and Basidiomycota.Comparative analysis revealed that fungi from Ascomycota and Basidiomycota differed in the number and variety of protein kinases.Some groups of protein kinases,such as calmodulin/calcium regulated kinases(CMGC) and those with the highest group percentages are the most prevalent protein kinases among all fungal species tested.In contrast,the STE group(homologs of the yeast STE7,STE11 and STE20 genes),was more abundant in Basidiomycetes than in Ascomycetes.Importantly,the distribution of some protein kinase families appeared to be subphylum-specific.The tyrosine kinase-like(TKL) group had a higher protein kinase density in Agaricomycotina fungi.In addition,the distribution of accessory domains,which could have functional implications,demonstrated that usage bias varied between the two phyla.Principal component analysis revealed a divergence between the main functional domains and associated domains in fungi.This study provides novel insights into the variety and expansion of fungal protein kinases between Ascomycota and Basidiomycota.展开更多
Diabetes is a chronic metabolic disease with an increasing incidence rate year by year.it severely diminishes the quality of life for individuals,reduces life expectancy,and poses a significant public health issue in ...Diabetes is a chronic metabolic disease with an increasing incidence rate year by year.it severely diminishes the quality of life for individuals,reduces life expectancy,and poses a significant public health issue in worldwide.Medicinal fungi,as a large group of microorganisms,possess abundant food and medicinal value,as well as vast research potential.This review describes the antidiabetic activities of seven medicinal fungi from subphylum Ascomycota,including Monascus purpureus,Cordyceps militaris,Ophiocordyceps sinensis,Cordyceps cicadae,Shiraia bambusicola,Claviceps purpurea,and Xylaria nigripes.it provides readers with evidence and information on the medicinal fungi(subphylum Ascomycota)with hypoglycemic effects,as well as their compounds for hypoglycemic properties.展开更多
The Yellow River Delta(YRD)of China is one of the most active land-sea interaction deltas in the world.However,due to human activities and climate change,it has undergone significant changes,including the degradation ...The Yellow River Delta(YRD)of China is one of the most active land-sea interaction deltas in the world.However,due to human activities and climate change,it has undergone significant changes,including the degradation of natural wetlands and saltwater intrusion.As an integral part of soil microorganisms,fungi play a crucial role in maintaining and stabilizing the function of wetland ecosystems.To better understand the composition and diversity changes of fungal communities along a salinity gradient in the YRD of China and their relationship with environmental factors,fungal diversity,abundance,and composition in the sediments of four typical vegetation communities spanning from the riverbank to the seaside were investigated.The results showed that the electrical conductivity(EC)increased significantly from the riverbank to the coastal area(P<0.05),but the levels of total nitrogen(TN),total carbon(TC),total sulfur(TS),available phosphorous(AP),and ammonium(NH_(4)^(+)-N)increased in Phragmites australis community and then experienced a significant decrease in Tamarix chinensis community and Suaeda salsa community(P<0.05).The alpha diversity(Shannon and Simpson indices)of the soil fungal community exhibited a negative correlation with EC.There was a significant alteration in the structure of the fungal community,primarily influenced by EC and NO_(3)^(-)-N.Ascomycota was found to be the most abundant phylum,and its relative abundance is positively correlated with pH and TS.The relative abundance of Sordariomycetes,the second-largest class of Ascomycota,reached 38.95%.Salinity was identified as the most important factor driving changes in soil fungal community composition.In summary,the fungal community changed significantly along the salinity gradient,and different environmental factors impacted various tiers of fungal populations differently.The findings of this study lay the groundwork for comprehending soil fungal communities and their primary influencing factors in newly formed wetlands.展开更多
Diaporthales is an important group of fungi widely distributed worldwide as endophytes,pathogens,and saprobes on the various plants.Here,we collected and isolated 209 strains of the Diaporthales and then employed morp...Diaporthales is an important group of fungi widely distributed worldwide as endophytes,pathogens,and saprobes on the various plants.Here,we collected and isolated 209 strains of the Diaporthales and then employed morphological characteristics and advanced techniques such as multigene phylogenetics,genomic phylogenetics,molecular clock estimates,and metabolic pathways annotations to explore the evolutionary diversification and metabolic pathways within the Diaporthales.Firstly,our study confirmed that Diaporthales occurred early with a mean stem age of 181.5 Mya and a mean crown age of 157.7 Mya.Secondly,two new families,Sinodisculaceae fam.nov.and Ternstroemiomycetaceae fam.nov.,were introduced based on morphology,phylogeny,and divergence times.Thirdly,we further described multiple novel taxa or records including Anadiaporthostoma gen.nov.(Diaporthostomataceae),Lunatospora gen.nov.(Sinodisculaceae),Microphaeotubakia gen.nov.(Tubakiaceae),Neoplagiostoma gen.nov.(Pseudoplagiostomataceae),and Ternstroemiomyces gen.nov.(Ternstroemiomycetaceae),55 new species,three new species complexes,32 new host records,and three new combinations.Furthermore,we accepted 35 families within the Diaporthales based on analysis of multiple evidences.Additionally,high activity in universal pathways such as purine metabolism and ribosome across the order suggested a fundamental for robust growth and stress response in Diaporthales.These findings enrich fungal biodiversity and provide critical insights into the evolutionary processes in these communities.展开更多
采集湖南省新宁县崀山世界自然遗产风景名胜区和广西省乐业县雅长兰科植物自然保护区这两个生境中处于生长期的铁皮石斛(Dendrobium of ficinale)的营养根,并进行分离培养。通过对分离所得的41个菌株进行形态观察和ITS序列测定相结合的...采集湖南省新宁县崀山世界自然遗产风景名胜区和广西省乐业县雅长兰科植物自然保护区这两个生境中处于生长期的铁皮石斛(Dendrobium of ficinale)的营养根,并进行分离培养。通过对分离所得的41个菌株进行形态观察和ITS序列测定相结合的鉴定,共获得内生真菌34种。对担子菌和子囊菌分别进行了系统发育树构建结果显示,子囊菌为优势种类(31种),以炭角菌目(Xylariales)和肉座菌目(Hypocreales)的种类为主,担子菌则以胶膜菌科(Tulasnellaceae)为主。Simpson多样性指数分析结果表明,不同生境下野生铁皮石斛内生真菌的多样性都很高,且雅长的高于崀山的。展开更多
基金supported by the National Science & Technology Pillar Program of China during the Twelfth Five-Year Plan period (2012BAD19B04)the National Natural Science Foundation of China (31371924)+1 种基金the 111 Project from the Ministry of Education of China (B07049)the National Basic Research Program of China (2013CB127700)
文摘Protein kinases play an important role in every aspect of cellular life.In this study,we systemically identified protein kinases from the predicted proteomes of 59 representative fungi from Ascomycota and Basidiomycota.Comparative analysis revealed that fungi from Ascomycota and Basidiomycota differed in the number and variety of protein kinases.Some groups of protein kinases,such as calmodulin/calcium regulated kinases(CMGC) and those with the highest group percentages are the most prevalent protein kinases among all fungal species tested.In contrast,the STE group(homologs of the yeast STE7,STE11 and STE20 genes),was more abundant in Basidiomycetes than in Ascomycetes.Importantly,the distribution of some protein kinase families appeared to be subphylum-specific.The tyrosine kinase-like(TKL) group had a higher protein kinase density in Agaricomycotina fungi.In addition,the distribution of accessory domains,which could have functional implications,demonstrated that usage bias varied between the two phyla.Principal component analysis revealed a divergence between the main functional domains and associated domains in fungi.This study provides novel insights into the variety and expansion of fungal protein kinases between Ascomycota and Basidiomycota.
基金funded by Shandong Provincial Natural Science Foundation(Zr2023Mc099)National Natural Science Foundation of china(21877075)Key research and Development Program of Shandong Province(2022TZXD0033).
文摘Diabetes is a chronic metabolic disease with an increasing incidence rate year by year.it severely diminishes the quality of life for individuals,reduces life expectancy,and poses a significant public health issue in worldwide.Medicinal fungi,as a large group of microorganisms,possess abundant food and medicinal value,as well as vast research potential.This review describes the antidiabetic activities of seven medicinal fungi from subphylum Ascomycota,including Monascus purpureus,Cordyceps militaris,Ophiocordyceps sinensis,Cordyceps cicadae,Shiraia bambusicola,Claviceps purpurea,and Xylaria nigripes.it provides readers with evidence and information on the medicinal fungi(subphylum Ascomycota)with hypoglycemic effects,as well as their compounds for hypoglycemic properties.
基金Under the auspices of the National Natural Science Foundation of China(No.42471111,42107419)the Natural Science Foundation of Shandong Province(No.ZR2023MD076)+2 种基金Talent Induction Program for Youth Innovation Teams in Colleges and Universities of Shandong Province(No.2022-2024)Youth Innovation Teams in Colleges and Universities of Shandong Province(No.2022KJ118)Jilin Provincial Department of Science and Technology Project(No.20230203174SF)。
文摘The Yellow River Delta(YRD)of China is one of the most active land-sea interaction deltas in the world.However,due to human activities and climate change,it has undergone significant changes,including the degradation of natural wetlands and saltwater intrusion.As an integral part of soil microorganisms,fungi play a crucial role in maintaining and stabilizing the function of wetland ecosystems.To better understand the composition and diversity changes of fungal communities along a salinity gradient in the YRD of China and their relationship with environmental factors,fungal diversity,abundance,and composition in the sediments of four typical vegetation communities spanning from the riverbank to the seaside were investigated.The results showed that the electrical conductivity(EC)increased significantly from the riverbank to the coastal area(P<0.05),but the levels of total nitrogen(TN),total carbon(TC),total sulfur(TS),available phosphorous(AP),and ammonium(NH_(4)^(+)-N)increased in Phragmites australis community and then experienced a significant decrease in Tamarix chinensis community and Suaeda salsa community(P<0.05).The alpha diversity(Shannon and Simpson indices)of the soil fungal community exhibited a negative correlation with EC.There was a significant alteration in the structure of the fungal community,primarily influenced by EC and NO_(3)^(-)-N.Ascomycota was found to be the most abundant phylum,and its relative abundance is positively correlated with pH and TS.The relative abundance of Sordariomycetes,the second-largest class of Ascomycota,reached 38.95%.Salinity was identified as the most important factor driving changes in soil fungal community composition.In summary,the fungal community changed significantly along the salinity gradient,and different environmental factors impacted various tiers of fungal populations differently.The findings of this study lay the groundwork for comprehending soil fungal communities and their primary influencing factors in newly formed wetlands.
基金supported by the National Natural Science Foundation of China(nos.U2002203,31750001).
文摘Diaporthales is an important group of fungi widely distributed worldwide as endophytes,pathogens,and saprobes on the various plants.Here,we collected and isolated 209 strains of the Diaporthales and then employed morphological characteristics and advanced techniques such as multigene phylogenetics,genomic phylogenetics,molecular clock estimates,and metabolic pathways annotations to explore the evolutionary diversification and metabolic pathways within the Diaporthales.Firstly,our study confirmed that Diaporthales occurred early with a mean stem age of 181.5 Mya and a mean crown age of 157.7 Mya.Secondly,two new families,Sinodisculaceae fam.nov.and Ternstroemiomycetaceae fam.nov.,were introduced based on morphology,phylogeny,and divergence times.Thirdly,we further described multiple novel taxa or records including Anadiaporthostoma gen.nov.(Diaporthostomataceae),Lunatospora gen.nov.(Sinodisculaceae),Microphaeotubakia gen.nov.(Tubakiaceae),Neoplagiostoma gen.nov.(Pseudoplagiostomataceae),and Ternstroemiomyces gen.nov.(Ternstroemiomycetaceae),55 new species,three new species complexes,32 new host records,and three new combinations.Furthermore,we accepted 35 families within the Diaporthales based on analysis of multiple evidences.Additionally,high activity in universal pathways such as purine metabolism and ribosome across the order suggested a fundamental for robust growth and stress response in Diaporthales.These findings enrich fungal biodiversity and provide critical insights into the evolutionary processes in these communities.
文摘采集湖南省新宁县崀山世界自然遗产风景名胜区和广西省乐业县雅长兰科植物自然保护区这两个生境中处于生长期的铁皮石斛(Dendrobium of ficinale)的营养根,并进行分离培养。通过对分离所得的41个菌株进行形态观察和ITS序列测定相结合的鉴定,共获得内生真菌34种。对担子菌和子囊菌分别进行了系统发育树构建结果显示,子囊菌为优势种类(31种),以炭角菌目(Xylariales)和肉座菌目(Hypocreales)的种类为主,担子菌则以胶膜菌科(Tulasnellaceae)为主。Simpson多样性指数分析结果表明,不同生境下野生铁皮石斛内生真菌的多样性都很高,且雅长的高于崀山的。
基金Supported by the National Natural Science Foundation of China(No.31270073)Ministry of Science and Technology of China for Fundamental Research(No.2013FY110400)
基金Supported by National Natural Science Foundation of China(31270066＆31750001) the fund of Shandong Provincial Education Association for International Exchanges
