In the present study, we investigated the role of reactive oxygen species（ROS） elevation induced by an anti-diabetic vanadium compound, vanadyl acetylacetonate（VO（acac）2）, in the regulation of lipolysis and gluc...In the present study, we investigated the role of reactive oxygen species（ROS） elevation induced by an anti-diabetic vanadium compound, vanadyl acetylacetonate（VO（acac）2）, in the regulation of lipolysis and glucose metabolism using differentiated 3T3L1 adipocytes as a model system. By confocal laser scanning microscopy, we found that VO（acac）2 induced ROS generation under high glucose stimulation, and the pretreatment of NADPH oxidase inhibitors could significantly reduce the elevated ROS level. Meanwhile, the decreased phosphorylated levels of AKT and the two key modulators of lipolysis（HSL and perilipin） were observed by western blot analysis. We also found that the contents of glycerol release were further reduced as well. In addition, the levels of key regulatory proteins, AS160 and GSK3β, in glucose metabolism pathway were correspondingly reduced. These findings demonstrated that ROS induced by vanadium compounds could act as a metabolic signal to activate AKT pathway to inhibit lipolysis and promote glucose transport and glycogen synthesis rather than by direct action by themselves. Our study contributed to elucidate the anti-diabetic effects of vanadium compounds and provided a theoretical basis for the further development of new vanadium complexes in the prevention and therapeutics of diabetes.展开更多
The effect of Azufrasin(yellow coat seed)and Flor de Junio(FJ)Dalia(pink-cream coat seed)cooked bean varieties was evaluated in high fat and fructose diet-fed rats to identify their health beneficial effects on obesit...The effect of Azufrasin(yellow coat seed)and Flor de Junio(FJ)Dalia(pink-cream coat seed)cooked bean varieties was evaluated in high fat and fructose diet-fed rats to identify their health beneficial effects on obesity-related metabolic alterations.FJ Dalia and Azufrasin daily supplementation(10%)for six months decreased body weight(5-7%),serum triglycerides(28.5-36.4%),HOMA insulin resistance index(59.6-62.3%),and serum TNF-α(40.5-45.0%);moreover,FJ Dalia increased serum adiponectin(77.5%).Polyphenol-,phytosterol-,and saponin-rich extracts from Azufrasin and FJ Dalia were evaluated in 3T3 L1 cells.Both varieties up-regulated,Cpt1a and Acadm(fatty acidβ-oxidation),and Glut4,Irs1,and Pi3k(insulin signaling cascade)and down-regulated Pparg and Cebpa(adipogenesis)and Fasn and Acaca(lipogenesis).These activities were associated with their high content of soyasaponins A3,Af,and Be for adipogenesis and lipid metabolism,as well as eriodictyol,ellagic acid,hesperidin and(+)-catechin and(-)-epicatechin and their derivatives,andβ-campesterol hexoside for insulin signaling cascade.FJ Dalia and Azufrasin cooked beans could be consumed to ameliorate obesity and its metabolic complications.展开更多
A series of 2 benzyl 1,3 dicabonyl derivatives was synthesized. Their insulin sensitizing activity was evaluated in 3T3 L1 preadipocyte cells. Compounds 3, 26 and 27 were found to possess strong insulin sens...A series of 2 benzyl 1,3 dicabonyl derivatives was synthesized. Their insulin sensitizing activity was evaluated in 3T3 L1 preadipocyte cells. Compounds 3, 26 and 27 were found to possess strong insulin sensitizing activity in vitro and were selected for further hypoglycemic evaluation in vivo.展开更多
基金National Natural Science Foundation of China(Grant No.20871008 and 21171011)
文摘In the present study, we investigated the role of reactive oxygen species（ROS） elevation induced by an anti-diabetic vanadium compound, vanadyl acetylacetonate（VO（acac）2）, in the regulation of lipolysis and glucose metabolism using differentiated 3T3L1 adipocytes as a model system. By confocal laser scanning microscopy, we found that VO（acac）2 induced ROS generation under high glucose stimulation, and the pretreatment of NADPH oxidase inhibitors could significantly reduce the elevated ROS level. Meanwhile, the decreased phosphorylated levels of AKT and the two key modulators of lipolysis（HSL and perilipin） were observed by western blot analysis. We also found that the contents of glycerol release were further reduced as well. In addition, the levels of key regulatory proteins, AS160 and GSK3β, in glucose metabolism pathway were correspondingly reduced. These findings demonstrated that ROS induced by vanadium compounds could act as a metabolic signal to activate AKT pathway to inhibit lipolysis and promote glucose transport and glycogen synthesis rather than by direct action by themselves. Our study contributed to elucidate the anti-diabetic effects of vanadium compounds and provided a theoretical basis for the further development of new vanadium complexes in the prevention and therapeutics of diabetes.
文摘The effect of Azufrasin(yellow coat seed)and Flor de Junio(FJ)Dalia(pink-cream coat seed)cooked bean varieties was evaluated in high fat and fructose diet-fed rats to identify their health beneficial effects on obesity-related metabolic alterations.FJ Dalia and Azufrasin daily supplementation(10%)for six months decreased body weight(5-7%),serum triglycerides(28.5-36.4%),HOMA insulin resistance index(59.6-62.3%),and serum TNF-α(40.5-45.0%);moreover,FJ Dalia increased serum adiponectin(77.5%).Polyphenol-,phytosterol-,and saponin-rich extracts from Azufrasin and FJ Dalia were evaluated in 3T3 L1 cells.Both varieties up-regulated,Cpt1a and Acadm(fatty acidβ-oxidation),and Glut4,Irs1,and Pi3k(insulin signaling cascade)and down-regulated Pparg and Cebpa(adipogenesis)and Fasn and Acaca(lipogenesis).These activities were associated with their high content of soyasaponins A3,Af,and Be for adipogenesis and lipid metabolism,as well as eriodictyol,ellagic acid,hesperidin and(+)-catechin and(-)-epicatechin and their derivatives,andβ-campesterol hexoside for insulin signaling cascade.FJ Dalia and Azufrasin cooked beans could be consumed to ameliorate obesity and its metabolic complications.
文摘A series of 2 benzyl 1,3 dicabonyl derivatives was synthesized. Their insulin sensitizing activity was evaluated in 3T3 L1 preadipocyte cells. Compounds 3, 26 and 27 were found to possess strong insulin sensitizing activity in vitro and were selected for further hypoglycemic evaluation in vivo.
