摘要
为研究丁香酚对3T3⁃L1前脂肪细胞成脂分化过程的影响及其分子机制,以未分化的3T3⁃L1前脂肪细胞为对照组,3⁃异丁基⁃1⁃甲基黄嘌呤、地塞米松、胰岛素和罗格列酮诱导分化的成熟脂肪细胞为模型组,探究3T3⁃L1细胞分化过程中的丁香酚处理对脂质积累的影响;使用腺苷酸环化酶抑制剂SQ22536处理细胞,探究环腺苷酸(cyclic adenosine monophosphate,cAMP)在丁香酚抑制脂质积累中发挥的关键作用。结果表明,与模型组相比,50µmol/L丁香酚处理显著降低脂质含量(P<0.0001),显著升高胞内cAMP水平(P<0.01)。当培养基中存在SQ22536时,丁香酚对3T3⁃L1细胞成脂分化过程中脂质积累的抑制作用由24.7%被削弱至6.7%,而丁香酚对细胞内cAMP水平的促进效果被完全消除。实时荧光定量聚合酶链式反应分析结果表明,丁香酚显著下调3T3⁃L1细胞成脂分化过程中脂质生成基因CEBPα(P<0.05)和aP2(P<0.01)的表达,显著上调产热相关基因UCP1的表达(P<0.01);SQ22536处理削弱或完全消除丁香酚对3T3⁃L1细胞脂质积累的抑制作用。因此,丁香酚可有效抑制3T3⁃L1细胞脂质积累,其作用机制与cAMP的激活有关。
This study aimed to investigate the effects and molecular mechanisms of eugenol on the adipogenic differentiation of 3T3⁃L1 preadipocytes.Undifferentiated 3T3⁃L1 preadipocytes were assigned to the control group,while mature adipocytes induced by 3⁃isobutyl⁃1⁃methylxanthine,dexamethasone,insulin,and rosigli⁃tazone were assigned to the model group.The impact of eugenol treatment on lipid accumulation during the dif⁃ferentiation process of 3T3⁃L1 cells was examined.Additionally,cells were treated with the adenylyl cyclase in⁃hibitor SQ22536 to explore the critical role of cyclic adenosine monophosphate(cAMP)in the inhibition of lipid accumulation by eugenol.The results demonstrated that compared to the model group,treatment with 50µmol/L eugenol significantly reduced lipid content(P<0.0001)and significantly increased intracellular cAMP levels(P<0.01).In the presence of SQ22536 in the medium,the inhibitory effect of eugenol on lipid ac⁃cumulation during 3T3⁃L1 cell differentiation was reduced from 24.7%to 6.7%,and the promotive effect of eu⁃genol on intracellular cAMP levels was completely abolished.Real⁃time quantitative polymerase chain reaction(PCR)analysis indicated that eugenol significantly downregulated the expression of lipidogenesis⁃related genes CEBPα(P<0.05)and aP2(P<0.01)during the adipogenic differentiation of 3T3⁃L1 cells,and signifi⁃cantly upregulated the expression of the thermogenesis⁃related gene UCP1(P<0.01).Treatment with SQ22536 attenuated or completely eliminated the inhibitory effect of eugenol on lipid accumulation in 3T3⁃L1 cells.Therefore,eugenol effectively inhibited lipid accumulation in 3T3⁃L1 cells,and its mechanism of action was as⁃sociated with the activation of cAMP.
作者
李梦杰
黄昆仑
仝涛
LI Mengjie;HUANG Kunlun;TONG Tao(College of Food Science and Nutritional Engineering,Key Laboratory of Precision Nutrition and Food Quality,Key Laboratory of Functional Dairy,Ministry of Education,China Agricultural University,Beijing 100083,China;Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),the Ministry of Agriculture and Rural Affairs,Beijing 100083,China;Beijing Laboratory for Food Quality and Safety,Beijing 100083,China)
出处
《食品研究与开发》
CAS
2024年第18期68-73,共6页
Food Research and Development
基金
北京市自然科学基金项目(7222249)。
