目的 探究16S rDNA不同区间对药品生产环境常见细菌的种水平鉴定能力。方法 计算德国细菌古菌分类学数据库(List of Prokaryotic names with Standing in Nomenclature,LPSN)收录的所有模式菌株序列以及洁净室中常见分离细菌对应的模式...目的 探究16S rDNA不同区间对药品生产环境常见细菌的种水平鉴定能力。方法 计算德国细菌古菌分类学数据库(List of Prokaryotic names with Standing in Nomenclature,LPSN)收录的所有模式菌株序列以及洁净室中常见分离细菌对应的模式菌株在16S rDNA不同区间香农熵,分析16S rDNA不同区间的物种水平鉴定能力;对所有LPSN收录的模式菌株进行相似度差值的比较,同时收集403个具有代表性的药品生产环境分离菌株样品,进行16S rDNA测序后完成相似度差值的比较,基于相似度差值探究16S rDNA不同区间的鉴定结果区分度;考虑到不同区间对不同种属可能存在区分能力的差异,对各区间测序区分度不显著的物种进行了相关性分析。结果 16S rDNA的27F~533R区间相比其他区间(533F~1100R区间、1100F~1492R区间)具有最高的种间分辨率,同时该区间的鉴定结果中,第一顺位与第二顺位的序列相似度差值最大,扩展比对区间对提升物种间的区分度没有显著效果。结论 优先应用16S rDNA的27F~533R区间开展细菌的种水平鉴定是高效、可靠的做法。展开更多
Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches...Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches.While the study represents a valuable methodological step forward,it remains limited by singlecenter design,lack of quantitative calibration,and insufficient control for contamination and inter-laboratory variability.This editorial critically appraises these methodological gaps and emphasizes that future efforts must focus on harmonized,consensus-driven workflows to ensure reproducibility and clinical reliability.The translational potential of multi-region 16S lies in moving from descriptive microbial profiling to actionable clinical integration,particularly for recurrence prediction,treatment-response monitoring,and perioperative complication risk assessment.By addressing these methodological,economic,and ethical challenges,the field can advance toward evidence-based and clinically deployable microbiome-guided precision oncology.展开更多
目的探讨淫羊藿苷对老年雌性与雄性小鼠肠道菌群的调节作用,评估其对菌群多样性、结构及代谢功能的影响,并分析性别差异在干预效果中的作用。方法选取年轻(2月龄)与老年(24月龄)雌雄C57BL/6J小鼠,分别灌胃淫羊藿苷(100 mg·kg·...目的探讨淫羊藿苷对老年雌性与雄性小鼠肠道菌群的调节作用,评估其对菌群多样性、结构及代谢功能的影响,并分析性别差异在干预效果中的作用。方法选取年轻(2月龄)与老年(24月龄)雌雄C57BL/6J小鼠,分别灌胃淫羊藿苷(100 mg·kg·d^(-1))或生理盐水,持续14 d。采用16S rRNA基因测序分析肠道菌群多样性及物种组成,结合京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析功能预测评估代谢通路变化。结果淫羊藿苷显著提高老年雌雄小鼠肠道菌群的α多样性(Chao1指数提升25%,P<0.05),并重塑菌群结构(β多样性PCoA分析显示老年组向年轻组趋近)。门水平上,老年小鼠拟杆菌门(Bacteroidetes)丰度降低,厚壁菌门(Firmicutes)丰度升高(F/B比值回调至年轻水平);科水平上,阿克曼氏菌科(Akkermansiaceae)在雌性老年小鼠中显著富集(4.1倍)。KEGG预测显示,淫羊藿苷改善老年小鼠糖代谢与鞘脂代谢(雌性组鞘脂通路活性上调3.2倍)。结论淫羊藿苷通过调节肠道菌群多样性及代谢功能延缓衰老,且作用具有性别二态性,为中药抗衰老的性别差异化干预提供了理论依据。展开更多
文摘Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches.While the study represents a valuable methodological step forward,it remains limited by singlecenter design,lack of quantitative calibration,and insufficient control for contamination and inter-laboratory variability.This editorial critically appraises these methodological gaps and emphasizes that future efforts must focus on harmonized,consensus-driven workflows to ensure reproducibility and clinical reliability.The translational potential of multi-region 16S lies in moving from descriptive microbial profiling to actionable clinical integration,particularly for recurrence prediction,treatment-response monitoring,and perioperative complication risk assessment.By addressing these methodological,economic,and ethical challenges,the field can advance toward evidence-based and clinically deployable microbiome-guided precision oncology.
文摘目的探讨淫羊藿苷对老年雌性与雄性小鼠肠道菌群的调节作用,评估其对菌群多样性、结构及代谢功能的影响,并分析性别差异在干预效果中的作用。方法选取年轻(2月龄)与老年(24月龄)雌雄C57BL/6J小鼠,分别灌胃淫羊藿苷(100 mg·kg·d^(-1))或生理盐水,持续14 d。采用16S rRNA基因测序分析肠道菌群多样性及物种组成,结合京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析功能预测评估代谢通路变化。结果淫羊藿苷显著提高老年雌雄小鼠肠道菌群的α多样性(Chao1指数提升25%,P<0.05),并重塑菌群结构(β多样性PCoA分析显示老年组向年轻组趋近)。门水平上,老年小鼠拟杆菌门(Bacteroidetes)丰度降低,厚壁菌门(Firmicutes)丰度升高(F/B比值回调至年轻水平);科水平上,阿克曼氏菌科(Akkermansiaceae)在雌性老年小鼠中显著富集(4.1倍)。KEGG预测显示,淫羊藿苷改善老年小鼠糖代谢与鞘脂代谢(雌性组鞘脂通路活性上调3.2倍)。结论淫羊藿苷通过调节肠道菌群多样性及代谢功能延缓衰老,且作用具有性别二态性,为中药抗衰老的性别差异化干预提供了理论依据。
