The effects of solvent and impurity on the crystal habit of 11α-hydroxy-16α,17α-epoxyprogesterone (HEP)grown from solution were studied by scanning electron microscope.Long prismatic crystals were produced when HEP...The effects of solvent and impurity on the crystal habit of 11α-hydroxy-16α,17α-epoxyprogesterone (HEP)grown from solution were studied by scanning electron microscope.Long prismatic crystals were produced when HEP was crystallized from pure acetone and N,N-dimethylformamide,while blocky crystals were produced from pure chloroform by cooling crystallization.One kind of isomorphic impurity,16α,17α-epoxyprogesterone(EP) was selected to examine its effect on the HEP crystal habit.When the content of EP in the mother liquor is very high(55.45%,solvent free basis),the habit of produced HEP crystals was greatly modified from prismatic to octa-hedral.The differential scanning calorimetry and X-ray powder diffraction analyses showed that the change of crystal habit was originated from the crystal structure modification.展开更多
To construct high-performance aqueous ammonium-ion full batteries,(NH_(4))_(2)V_(6)O_(16)·1.5H_(2)O(NVO)nanoribbon cathodes were prepared by pH-regulated hydrothermal synthesis.Anodes were prepared by growing the...To construct high-performance aqueous ammonium-ion full batteries,(NH_(4))_(2)V_(6)O_(16)·1.5H_(2)O(NVO)nanoribbon cathodes were prepared by pH-regulated hydrothermal synthesis.Anodes were prepared by growing the active material polyaniline(PANI)on carbon cloth.The assembled NVO//PANI full cells exhibit a reversible capacity of 109.5 mA·h/g at a current density of 1.0 A/g and a high energy density of 23 W·h/kg.The ammonium-ion intercalation/extraction mechanism is primarily governed by the pseudocapacitance behavior.These results indicate that NVO is a potential candidate as a cathode material for aqueous ammonium-ion batteries.展开更多
甲基化酶样16(Methyltransferase like 16,METTL16)作为N6-甲基腺苷(N6-methyladenosine,m6A)甲基转移酶家族重要成员,通过表观遗传修饰动态调控肿瘤发生发展。尽管METTL16在多种肿瘤中的重要性已得到证实,但目前对其在不同肿瘤类型中...甲基化酶样16(Methyltransferase like 16,METTL16)作为N6-甲基腺苷(N6-methyladenosine,m6A)甲基转移酶家族重要成员,通过表观遗传修饰动态调控肿瘤发生发展。尽管METTL16在多种肿瘤中的重要性已得到证实,但目前对其在不同肿瘤类型中的分子机制异质性和功能多样性仍缺乏系统性的总结分析。本文基于最新研究进展,全面梳理了METTL16在肿瘤发生发展中的分子机制及其临床转化价值。研究表明,METTL16可通过m6A依赖性和非依赖性双重机制调控下游靶基因网络,进而影响肿瘤细胞周期进程、代谢重编程和铁死亡抵抗等关键生物学过程。研究显示,METTL16表达水平与一些肿瘤如胃癌、肝细胞癌、结直肠癌的预后明显相关。在治疗策略方面,靶向METTL16的干预措施已取得初步进展,包括通过对聚腺苷二磷酸核糖聚合酶抑制剂(Poly adp-ribose polymerase inhibitors,PARPi)增敏加强胰腺导管腺癌治疗效果,以及提高膀胱癌对顺铂化疗敏感性等。这些研究不仅揭示了METTL16作为肿瘤治疗关键靶点的重要价值,也为开发新型抗肿瘤策略提供了理论依据。展开更多
文摘The effects of solvent and impurity on the crystal habit of 11α-hydroxy-16α,17α-epoxyprogesterone (HEP)grown from solution were studied by scanning electron microscope.Long prismatic crystals were produced when HEP was crystallized from pure acetone and N,N-dimethylformamide,while blocky crystals were produced from pure chloroform by cooling crystallization.One kind of isomorphic impurity,16α,17α-epoxyprogesterone(EP) was selected to examine its effect on the HEP crystal habit.When the content of EP in the mother liquor is very high(55.45%,solvent free basis),the habit of produced HEP crystals was greatly modified from prismatic to octa-hedral.The differential scanning calorimetry and X-ray powder diffraction analyses showed that the change of crystal habit was originated from the crystal structure modification.
文摘目的 本研究拟通过体外实验研究槲皮素(Quercetin,QCT)与AC16心肌细胞增殖的关系,探析QCT阻止AC16心肌细胞增殖作用与Wnt/β-catenin信号通路的关系。方法 选用不同浓度QCT对AC16细胞进行干预,使用倒置显微镜拍照观察法、台盼蓝计数法和CCK-8法检测QCT对AC16细胞增殖的影响。Western blot检测QCT对磷酸化-β-连环蛋白(Phosphorylated β-catenin,p-β-catenin)、细胞性骨髓瘤样癌基因(c-Myc)、β-连环蛋白(β-catenin)、低密度脂蛋白受体相关蛋白5(Low density lipoprotein receptor-related protein 5,LRP5)和低密度脂蛋白受体相关蛋白6(Low density lipoprotein receptor-related protein 6,LRP6)表达的影响。台盼蓝计数法检测过表达β-catenin ΔN、LRP5和LRP6基因后研究QCT对细胞增殖的影响以及沉默LRP5和LRP6基因后研究QCT对细胞增殖的影响。结果 与对照组比,QCT使得AC16细胞活细胞数下降,抑制增殖率,且具有浓度依赖性。条件摸索显示10和20μmol/L的QCT会导致AC16细胞中p-β-catenin蛋白修饰水平显著增加(P<0.05),c-Myc、β-catenin、LRP5和LRP6蛋白表达水平显著下调(P<0.05),因此后续实验选择10μmol/L的QCT作为干预浓度。而AC16细胞中过表达β-catenin ΔN、LRP5和LRP6基因,与单独给药组相比,过表达相关基因后10μmol/L的QCT引起的细胞增殖抑制现象被有效逆转(P<0.05)。另外,沉默LRP5和LRP6再联合10μmol/L的QCT干预,AC16细胞的增殖抑制并未产生协同现象(P>0.05)。结论 QCT可以通过下调LRP5/6表达以抑制Wnt/β-catenin信号通路从而发挥其阻止AC16心肌细胞增殖的作用。
基金supported by the National Natural Science Foundation of China(Nos.52171200,52371211)the Changsha Special Project,China(No.kh2301006)。
文摘To construct high-performance aqueous ammonium-ion full batteries,(NH_(4))_(2)V_(6)O_(16)·1.5H_(2)O(NVO)nanoribbon cathodes were prepared by pH-regulated hydrothermal synthesis.Anodes were prepared by growing the active material polyaniline(PANI)on carbon cloth.The assembled NVO//PANI full cells exhibit a reversible capacity of 109.5 mA·h/g at a current density of 1.0 A/g and a high energy density of 23 W·h/kg.The ammonium-ion intercalation/extraction mechanism is primarily governed by the pseudocapacitance behavior.These results indicate that NVO is a potential candidate as a cathode material for aqueous ammonium-ion batteries.
