[Objectives]To determine the content of hyperin and luteolin in Vernonia cinerea(L.)Less.,and to provide basic experimental data for experimental research and clinical application of V.cinerea(L.)Less.[Methods]The com...[Objectives]To determine the content of hyperin and luteolin in Vernonia cinerea(L.)Less.,and to provide basic experimental data for experimental research and clinical application of V.cinerea(L.)Less.[Methods]The components to be determined were extracted by ultrasonic extraction,and the hyperin and luteolin in V.cinerea(L.)Less.were separated and determined by reversed-phase high performance liquid chromatography(chromatographic conditions:reverse C 18 column,methanol-0.4%phosphoric acid as mobile phase for gradient elution,flow rate of 1.0 mL/min,detector wavelength of 360 nm).[Results]The precision and repeatability of the experimental method were good,and the solution of V.cinerea(L.)Less.was stable within 18 h.There was a good linear relationship between the injection volume of hyperin and luteolin and the chromatographic peak area within the prescribed range,and the recovery rate was qualified.The content of hyperin and luteolin in V.cinerea(L.)Less.was 0.0665 mg/g(n=3)and 0.1004 mg/g(n=3),respectively.[Conclusions]The experimental method is sensitive,specific,stable,accurate and reliable,and can be used for the determination of hyperin and luteolin in V.cinerea(L.)Less.展开更多
As one of the cultural heritages of the nation,Mongolian Medicine was formed and developed in the long-term medical practice of the Mongolian people,and has become a major constituent part in the Traditional Chinese M...As one of the cultural heritages of the nation,Mongolian Medicine was formed and developed in the long-term medical practice of the Mongolian people,and has become a major constituent part in the Traditional Chinese Medicine.In the past,Mongolian medicine made great contributions to the living and breeding of generations of the Mongolian people and had a positive impact on the formation of展开更多
Four constituents were isolated from Rhododendron micranthum Turcz . Their structures were identified ashyperin ,astragalin ,kaempferol ,and quercetin . Kaempferol wasisolated from the plantforthe firsttime . Hyperin ...Four constituents were isolated from Rhododendron micranthum Turcz . Their structures were identified ashyperin ,astragalin ,kaempferol ,and quercetin . Kaempferol wasisolated from the plantforthe firsttime . Hyperin wasthe main analgesic active constituent.展开更多
基金Supported by Independent Research Project of Key Laboratory of Extraction,Purification and Quality Analysis of Traditional Chinese Medicine in Guangxi Universities(J1606701).
文摘[Objectives]To determine the content of hyperin and luteolin in Vernonia cinerea(L.)Less.,and to provide basic experimental data for experimental research and clinical application of V.cinerea(L.)Less.[Methods]The components to be determined were extracted by ultrasonic extraction,and the hyperin and luteolin in V.cinerea(L.)Less.were separated and determined by reversed-phase high performance liquid chromatography(chromatographic conditions:reverse C 18 column,methanol-0.4%phosphoric acid as mobile phase for gradient elution,flow rate of 1.0 mL/min,detector wavelength of 360 nm).[Results]The precision and repeatability of the experimental method were good,and the solution of V.cinerea(L.)Less.was stable within 18 h.There was a good linear relationship between the injection volume of hyperin and luteolin and the chromatographic peak area within the prescribed range,and the recovery rate was qualified.The content of hyperin and luteolin in V.cinerea(L.)Less.was 0.0665 mg/g(n=3)and 0.1004 mg/g(n=3),respectively.[Conclusions]The experimental method is sensitive,specific,stable,accurate and reliable,and can be used for the determination of hyperin and luteolin in V.cinerea(L.)Less.
文摘As one of the cultural heritages of the nation,Mongolian Medicine was formed and developed in the long-term medical practice of the Mongolian people,and has become a major constituent part in the Traditional Chinese Medicine.In the past,Mongolian medicine made great contributions to the living and breeding of generations of the Mongolian people and had a positive impact on the formation of
文摘Four constituents were isolated from Rhododendron micranthum Turcz . Their structures were identified ashyperin ,astragalin ,kaempferol ,and quercetin . Kaempferol wasisolated from the plantforthe firsttime . Hyperin wasthe main analgesic active constituent.
