Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches...Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches.While the study represents a valuable methodological step forward,it remains limited by singlecenter design,lack of quantitative calibration,and insufficient control for contamination and inter-laboratory variability.This editorial critically appraises these methodological gaps and emphasizes that future efforts must focus on harmonized,consensus-driven workflows to ensure reproducibility and clinical reliability.The translational potential of multi-region 16S lies in moving from descriptive microbial profiling to actionable clinical integration,particularly for recurrence prediction,treatment-response monitoring,and perioperative complication risk assessment.By addressing these methodological,economic,and ethical challenges,the field can advance toward evidence-based and clinically deployable microbiome-guided precision oncology.展开更多
目的探讨淫羊藿苷对老年雌性与雄性小鼠肠道菌群的调节作用,评估其对菌群多样性、结构及代谢功能的影响,并分析性别差异在干预效果中的作用。方法选取年轻(2月龄)与老年(24月龄)雌雄C57BL/6J小鼠,分别灌胃淫羊藿苷(100 mg·kg·...目的探讨淫羊藿苷对老年雌性与雄性小鼠肠道菌群的调节作用,评估其对菌群多样性、结构及代谢功能的影响,并分析性别差异在干预效果中的作用。方法选取年轻(2月龄)与老年(24月龄)雌雄C57BL/6J小鼠,分别灌胃淫羊藿苷(100 mg·kg·d^(-1))或生理盐水,持续14 d。采用16S rRNA基因测序分析肠道菌群多样性及物种组成,结合京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析功能预测评估代谢通路变化。结果淫羊藿苷显著提高老年雌雄小鼠肠道菌群的α多样性(Chao1指数提升25%,P<0.05),并重塑菌群结构(β多样性PCoA分析显示老年组向年轻组趋近)。门水平上,老年小鼠拟杆菌门(Bacteroidetes)丰度降低,厚壁菌门(Firmicutes)丰度升高(F/B比值回调至年轻水平);科水平上,阿克曼氏菌科(Akkermansiaceae)在雌性老年小鼠中显著富集(4.1倍)。KEGG预测显示,淫羊藿苷改善老年小鼠糖代谢与鞘脂代谢(雌性组鞘脂通路活性上调3.2倍)。结论淫羊藿苷通过调节肠道菌群多样性及代谢功能延缓衰老,且作用具有性别二态性,为中药抗衰老的性别差异化干预提供了理论依据。展开更多
文摘Wu et al recently applied multi-region 16S rRNA sequencing to characterize the gastric cancer microbiome,demonstrating improved taxonomic resolution and detection sensitivity over conventional single-region approaches.While the study represents a valuable methodological step forward,it remains limited by singlecenter design,lack of quantitative calibration,and insufficient control for contamination and inter-laboratory variability.This editorial critically appraises these methodological gaps and emphasizes that future efforts must focus on harmonized,consensus-driven workflows to ensure reproducibility and clinical reliability.The translational potential of multi-region 16S lies in moving from descriptive microbial profiling to actionable clinical integration,particularly for recurrence prediction,treatment-response monitoring,and perioperative complication risk assessment.By addressing these methodological,economic,and ethical challenges,the field can advance toward evidence-based and clinically deployable microbiome-guided precision oncology.
文摘目的探讨淫羊藿苷对老年雌性与雄性小鼠肠道菌群的调节作用,评估其对菌群多样性、结构及代谢功能的影响,并分析性别差异在干预效果中的作用。方法选取年轻(2月龄)与老年(24月龄)雌雄C57BL/6J小鼠,分别灌胃淫羊藿苷(100 mg·kg·d^(-1))或生理盐水,持续14 d。采用16S rRNA基因测序分析肠道菌群多样性及物种组成,结合京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析功能预测评估代谢通路变化。结果淫羊藿苷显著提高老年雌雄小鼠肠道菌群的α多样性(Chao1指数提升25%,P<0.05),并重塑菌群结构(β多样性PCoA分析显示老年组向年轻组趋近)。门水平上,老年小鼠拟杆菌门(Bacteroidetes)丰度降低,厚壁菌门(Firmicutes)丰度升高(F/B比值回调至年轻水平);科水平上,阿克曼氏菌科(Akkermansiaceae)在雌性老年小鼠中显著富集(4.1倍)。KEGG预测显示,淫羊藿苷改善老年小鼠糖代谢与鞘脂代谢(雌性组鞘脂通路活性上调3.2倍)。结论淫羊藿苷通过调节肠道菌群多样性及代谢功能延缓衰老,且作用具有性别二态性,为中药抗衰老的性别差异化干预提供了理论依据。
