Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were ana...Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were analyzed by PCR with two kinds of primers.The results showed that in the electrophoresis pattern of the PCR products the somatic hybrids had the characteristic bands of two parents and (or) new bands.This research reveals that PCR analysis with 5S rDNA spacer sequence primers can be used for the identification of somatic hybrids at the molecular level and it is a good method because of its simplicity and good reproducibility.展开更多
文摘Three intergeneric somatic hybrids (wheat(+) Haynaldia villosa, wheat(+) Bromus inermis, wheat(+) Agropyron elongatum) and one interfamily somatic hybrid between Vitis vinifera and Bupleurum scorzonerifollium were analyzed by PCR with two kinds of primers.The results showed that in the electrophoresis pattern of the PCR products the somatic hybrids had the characteristic bands of two parents and (or) new bands.This research reveals that PCR analysis with 5S rDNA spacer sequence primers can be used for the identification of somatic hybrids at the molecular level and it is a good method because of its simplicity and good reproducibility.
