摘要
目的 研究金银花药材道地性形成的基因基础。方法 用 SDS法提取金银花 L onicera japonica不同居群、外类群细毡毛忍冬 L.similis和山银花 L.confusa的总 DNA,进行 5 S- r RNA基因间区的 PCR扩增和测序 ,并用软件 Mega进行分析。结果 L onicera L.属植物 5 S- r RNA基因间区约 2 10 bp,其中 G+C含量较高 ,达 70 %左右 ,不同居群的 L.japonica碱基序列有差别 ,通过测序可以进行鉴别。L.confusa与 L.japonica间的遗传距离较大。结论 种间 5 S- r RNA基因间区的序列差异大于种内 ;道地药材之间的遗传距离较小 ;道地与非道地药材之间的遗传距离大于道地药材之间的遗传距离。
Object To study the formation of geoherbalism of Flos Lonicerae on the molecular level Methods Genomic DNAs from different populations of Lonicera japonica Thunb , outgroup Lonicera similis Hemsl , and Lonicera confusa DC were extracted, the 5S rRNA gene spacer region amplified, sequuenced, and analysed with Mega 1 02 Results The fragments of 5S rRNA gene spacer region in Lonicera L was about 210 bp with G+C content up to about 70% The sequences were different in various populations, and can be identified by sequencing The genetic distance between L confusa and L japonica was larger Conclusion The genuine distance between species was larger than that within species; the genetic distance among genuine crude drugs was smaller; the genetic distance among genuine and ungenuine crude drugs were bigger than that among genuine crude drugs
出处
《中草药》
CAS
CSCD
北大核心
2001年第9期834-837,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金重点项目 ( 39730 5 0 0 )
教育部优秀教师资助计划项目
江苏省"333"工程项目资助
