竹红菌素在医药、食品和化工领域有着广泛的应用,高效经济的制备方法是其产业化的基础。试验采用单因子比较的方法考察微波辅助方法对固体发酵基质中竹红菌素提取效率的影响。结果表明,当无水乙醇和竹黄菌的料液比为1∶75,反应温度50℃...竹红菌素在医药、食品和化工领域有着广泛的应用,高效经济的制备方法是其产业化的基础。试验采用单因子比较的方法考察微波辅助方法对固体发酵基质中竹红菌素提取效率的影响。结果表明,当无水乙醇和竹黄菌的料液比为1∶75,反应温度50℃,提取时间7 m in,微波功率600 W时提取效果最佳,竹红菌素的提取率达到0.1%。微波辅助方法能显著提高固体发酵基质中竹红菌素的提取得率。展开更多
Aim To synthetize 6-chloro-8-fluoro-4-methylumbelliferone phosphate(CF-MUP) and utilize it to analyze acid phosphatase activity.Method CF-MUP obtained by phosphorylation of CFMU(6-chloro-8-fluoro-4-methylumbelliferone...Aim To synthetize 6-chloro-8-fluoro-4-methylumbelliferone phosphate(CF-MUP) and utilize it to analyze acid phosphatase activity.Method CF-MUP obtained by phosphorylation of CFMU(6-chloro-8-fluoro-4-methylumbelliferone) which was prepared by Pechmann reaction between 4 and ethyl acetoacetate in the presence of methanesulfonic acid.And then,the utility of CF-MUP as a substrate for acid phosphatase was compared with MUP(4-methylumbelliferone phosphate).Results The new coumarin phosphate,CF-MUP,demonstrated much higher sensitivity and was more robust for detecting the activity of acid phosphatase than MUP in the physiological pH range.Conclusion This new fluorescence dye,CF-MUP,is a convenient tool for the continuous assay of acid phosphatases with a wide range of pH.展开更多
A filamwntous fungus Hypomyces(Fr) Tul. sp has been isolated from the western mountain area of Yunnan province in China and cultured successfully. The biocatalytic synthesis of fungus was investigated for the first ti...A filamwntous fungus Hypomyces(Fr) Tul. sp has been isolated from the western mountain area of Yunnan province in China and cultured successfully. The biocatalytic synthesis of fungus was investigated for the first time. Chemical components of fungus metabolite have been analyzed. The result shows that the metabolite products of fungus contain perylenequinones photosensitive pigments Elsinochrome A(EA), Hypocrellin A(HA), Hypocrellin B(HB), and mennitol. the chemical structure of perylenequinones were confirmed by elemental analysis, UV, FT IR, ( 1H, 13 C)2DNMR, MS, and X ray diffraction methods.展开更多
文摘竹红菌素在医药、食品和化工领域有着广泛的应用,高效经济的制备方法是其产业化的基础。试验采用单因子比较的方法考察微波辅助方法对固体发酵基质中竹红菌素提取效率的影响。结果表明,当无水乙醇和竹黄菌的料液比为1∶75,反应温度50℃,提取时间7 m in,微波功率600 W时提取效果最佳,竹红菌素的提取率达到0.1%。微波辅助方法能显著提高固体发酵基质中竹红菌素的提取得率。
基金Supported by the Phytochemistry Key Laboratory Foundation of Shaanxi Province(2010JS068)the Science Research Foundation of Baoji University of Arts and Sciences(ZK1040)
文摘Aim To synthetize 6-chloro-8-fluoro-4-methylumbelliferone phosphate(CF-MUP) and utilize it to analyze acid phosphatase activity.Method CF-MUP obtained by phosphorylation of CFMU(6-chloro-8-fluoro-4-methylumbelliferone) which was prepared by Pechmann reaction between 4 and ethyl acetoacetate in the presence of methanesulfonic acid.And then,the utility of CF-MUP as a substrate for acid phosphatase was compared with MUP(4-methylumbelliferone phosphate).Results The new coumarin phosphate,CF-MUP,demonstrated much higher sensitivity and was more robust for detecting the activity of acid phosphatase than MUP in the physiological pH range.Conclusion This new fluorescence dye,CF-MUP,is a convenient tool for the continuous assay of acid phosphatases with a wide range of pH.
文摘A filamwntous fungus Hypomyces(Fr) Tul. sp has been isolated from the western mountain area of Yunnan province in China and cultured successfully. The biocatalytic synthesis of fungus was investigated for the first time. Chemical components of fungus metabolite have been analyzed. The result shows that the metabolite products of fungus contain perylenequinones photosensitive pigments Elsinochrome A(EA), Hypocrellin A(HA), Hypocrellin B(HB), and mennitol. the chemical structure of perylenequinones were confirmed by elemental analysis, UV, FT IR, ( 1H, 13 C)2DNMR, MS, and X ray diffraction methods.
