摘要
Aim To synthetize 6-chloro-8-fluoro-4-methylumbelliferone phosphate(CF-MUP) and utilize it to analyze acid phosphatase activity.Method CF-MUP obtained by phosphorylation of CFMU(6-chloro-8-fluoro-4-methylumbelliferone) which was prepared by Pechmann reaction between 4 and ethyl acetoacetate in the presence of methanesulfonic acid.And then,the utility of CF-MUP as a substrate for acid phosphatase was compared with MUP(4-methylumbelliferone phosphate).Results The new coumarin phosphate,CF-MUP,demonstrated much higher sensitivity and was more robust for detecting the activity of acid phosphatase than MUP in the physiological pH range.Conclusion This new fluorescence dye,CF-MUP,is a convenient tool for the continuous assay of acid phosphatases with a wide range of pH.
Aim To synthetize 6-chlor0-8-fluoro-4-methylumbelliferone, phosphate （CF-MUP） and utilize it to analyze acid phosphatase activity. Method CF-MUP obtained by phosphorylation of CF- MU（6-chloro-8-fluoro-4-methylumbelliferone） which was prepared by Pechmann reaction between 4 and ethyl acetoacetate in the presence of methanesulfonic acid. And then, the utility of CF-MUP as asubstrate for acid phosphatase was compared with MUP（4-methylumbelliferone phosphate）. Results The new coumarin phosphate, CF-MUP, demonstrated much higher sensitivity and was more robust for detecting the activity of acid phosphatase than MUP in the physiological pH range. Conclusion This new fluorescence dye, CF-MUP, is a convenient tool for the continuous assay of acid phosphatases with a wide range of pH.
出处
《宝鸡文理学院学报（自然科学版）》
CAS
2012年第1期1-4,共4页
Journal of Baoji University of Arts and Sciences(Natural Science Edition)
基金
Supported by the Phytochemistry Key Laboratory Foundation of Shaanxi Province(2010JS068)
the Science Research Foundation of Baoji University of Arts and Sciences(ZK1040)
