In recent years, the research advancements have high-lighted the critical role of the A-site cation in determining the optoelectronic and physicochemical properties of organicinorganic lead halide perovskites. Mixed-c...In recent years, the research advancements have high-lighted the critical role of the A-site cation in determining the optoelectronic and physicochemical properties of organicinorganic lead halide perovskites. Mixed-cation perovskites(MCPs) have been extensively used as absorber thin films in perovskite solar cells(PSCs), achieving high power conversion efficiencies(PCE) over 26%^([1, 2]).展开更多
To achieve the target of carbon neutrality,it is crucial to develop an efficient and green synthesis methodology with good atomic economy to achieve sufficient utilization of energy and sustainable development.Photoin...To achieve the target of carbon neutrality,it is crucial to develop an efficient and green synthesis methodology with good atomic economy to achieve sufficient utilization of energy and sustainable development.Photoinduced electron transfer reversible addition-fragmentation chain-transfer(PET-RAFT)polymerization is a precise methodology for constructing polymers with well-defined structures.However,conventional semiconductor-mediated PET-RAFT polymerization still has considerable limitations in terms of efficiency as well as the polymerization environment.Herein,sulfur-doped carbonized polymer dots(CPDs)were hydrothermally synthesized for catalysis of aqueous PET-RAFT polymerization at unprecedented efficiency with a highest propagation rate of 5.05 h-1.The resulting polymers have well-controlled molecular weight and narrow molecular weight dispersion(Ð<1.10).Based on the optoelectronic characterizations,we obtained insights into the photoinduced electron transfer process and proposed the mechanism for CPD-mediated PET-RAFT polymerization.In addition,as-synthesized CPDs for PET-RAFT polymerization were also demonstrated to be suitable for a wide range of light sources(blue/green/solar irradiation),numerous monomers,low catalyst loading(low as 0.01 mg mL^(-1)),and multiple polar solvent environments,all of which allowed to achieve efficiencies much higher than those of existing semiconductor-mediated methods.Finally,the CPDs were confirmed to be non-cytotoxic and catalyzed PET-RAFT polymerization successfully in cell culture media,indicating broad prospects in biomedical fields.展开更多
<div style="text-align:justify;"> Dual polarization quadrature phase shift keying (DP-QPSK) is currently the main modulation format for high-speed optical fiber communications, and its simulation model...<div style="text-align:justify;"> Dual polarization quadrature phase shift keying (DP-QPSK) is currently the main modulation format for high-speed optical fiber communications, and its simulation modeling has important implications for theoretical research and practical applications. Based on Optisystem 15 optical communication system simulation software, this paper builds a 100 Gbit/s rate DP-QPSK modulation format coherent optical transmission system simulation model, analyzes the working principles of the transmitting end, transmission link, and coherent receiver. The principle of signal recovery at the digital signal processing end is briefly explained. The simulation results show that the signal quality after DSP processing has been significantly improved. </div>展开更多
Neural stem cells(NSCs)play a fundamental role in generating diverse neuronal populations that contribute to the formation of intricate neural circuitry.Disturbances arising from intrinsic or extrinsic factors can alt...Neural stem cells(NSCs)play a fundamental role in generating diverse neuronal populations that contribute to the formation of intricate neural circuitry.Disturbances arising from intrinsic or extrinsic factors can alter the developmental behavior of NSCs and disrupt nervous system homeostasis.While intrinsic regulatory mechanisms have been elucidated extensively in invertebrate or vertebrate models,the regulatory mechanisms underlying extrinsic cues from the cellular environment remain poorly understood.This review synthesized recent research on cellular ambient effects,including the microenvironment,systemic environment and external factors,on NSCs in Drosophila.Key topics include spatial cues,NSC-glia interactions,long-distance regulation by tissues such as the fat body,and the external environmental stressors like irradiation or viral infection.By integrating these findings,this review provides new insights into how extrinsic signals shape NSCs and bridges gaps between foundational research and clinical translation.展开更多
Schottky mass spectrometry utilizing heavy-ion storage rings is a powerful technique for the precise mass and decay half-life measurements of highly charged ions.Owing to the nondestructive ion detection features of S...Schottky mass spectrometry utilizing heavy-ion storage rings is a powerful technique for the precise mass and decay half-life measurements of highly charged ions.Owing to the nondestructive ion detection features of Schottky noise detectors,the number of stored ions in the ring is determined by the peak area in the measured revolution frequency spectrum.Because of their intrinsic amplitude-frequency characteristic(AFC),Schottky detector systems exhibit varying sensitivities at different frequencies.Using low-energy electron-cooled stored ions,a new method is developed to calibrate the AFC curve of the Schottky detector system of the Experimental Cooler Storage Ring(CSRe)storage ring located in Lanzhou,China.Using the amplitude-calibrated frequency spectrum,a notable refinement was observed in the precision of both the peak position and peak area.As a result,the storage lifetimes of the electron-cooled fully ionized^(56)Fe^(26+)ions were determined with high precision at beam energies of 13.7 and 116.4 MeV/u,despite of frequency drifts during the experiment.When electron cooling was turned off,the effective vacuum condition experienced by the 116.4 MeV/u^(56)Fe^(26+)ions was determined using amplitude-calibrated spectra,revealing a value of 2×10^(−10)mbar,which is consistent with vacuum gauge readings along the CSRe ring.The method reported herein will be adapted for the next-generation storage ring of the HIAF facility under construction in Huizhou,China.It can also be adapted to other storage ring facilities worldwide to improve precision and enhance lifetime measurements using many ions in the ring.展开更多
Cadmium(Cd)contamination in soil can lead to food chain accumulation and greatly impacts on human health.Bioremediation has gained more and more attention due to its environment-friendly,high efficiency and low-cost.I...Cadmium(Cd)contamination in soil can lead to food chain accumulation and greatly impacts on human health.Bioremediation has gained more and more attention due to its environment-friendly,high efficiency and low-cost.In this work,we studied the impact of phosphate solubilizing bacterial agent(PSBA)on Cd bioavailability,microbial communities in soil and Cd accumulation in lettuce plants with pot experiment and field trial.Results of pot experiment showed that PSBA could decrease the bioavailability of Cd(Cd-acid extractable from 3.30 to 2.34mg/kg,Cd-reducible from 1.94 to 1.56 mg/kg),promote lettuce plants growth(increased by 33.85%height and by 33.65%fresh weight)and reduce the accumulation of Cd(from 5.85 to 3.73 mg/kg)in lettuce plants.High-throughput sequencing identified that PSBA could change the composition and structure of the soil microbial communities.The relative abundances of the three ecologically beneficial bacterial families of Pseudomonadaceae,Burkholderiaceae,and Enterobacteriaceae increased from 2.29%to 5.13%,0.56%to 5.24%,and 1.87%to 16.93%,respectively.And the former two were positively correlated with redox potential(Eh)(R^(2)=0.474,p<0.05,R^(2)=0.590,p<0.01,respectively).The bacterial networks were more complex in PSBA treatment,reflecting through more links(from 1893 to 2185)and a higher average degree(from 38.242 to 45.052)and density(from 0.390 to 0.469).Results of field trial demonstrated that PSBA could also decrease Cd content in lettuce plants and microbial composition in soil.This study indicated that PSBA could be served as an alternative material in bioremediation of Cd contamination in soil.展开更多
BACKGROUND Emerging evidence indicates that hypoxic preconditioning boosts the antioxidant and anti-apoptotic capacities of mesenchymal stem cell-derived exosomes;however,the specific mechanisms remain incompletely el...BACKGROUND Emerging evidence indicates that hypoxic preconditioning boosts the antioxidant and anti-apoptotic capacities of mesenchymal stem cell-derived exosomes;however,the specific mechanisms remain incompletely elucidated.This study explored the impact of hypoxia-preconditioned mesenchymal stem cell-derived exosomes(hypo-Exos)vs normoxic counterparts on the apoptotic response in cardiomyocytes triggered by oxidative stress.AIM To determine whether and how hypoxic preconditioning augments the cardioprotective efficacy of hypo-Exos against oxidative stress-induced cardiomyocyte apoptosis.METHODS H9C2 cardiomyocytes were treated with hydrogen peroxide(H2O2)to induce oxidative injury.Assessments of cell viability,oxidative biomarkers,and apoptotic activity were conducted to evaluate the therapeutic efficacy of hypo-Exos and normoxic counterparts.High-throughput sequencing was performed to identify potential target microRNAs(miRNAs).Luciferase reporter assays were conducted to confirm selected miRNAs binding to target genes.Hypo-Exos loaded with selected miRNAs antagomirs or negative controls were administered to H2O2-treated H9C2 cells to validate the downstream signaling pathways involved.RESULTS Hypo-Exos significantly enhanced cell viability,reduced oxidative stress,and inhibited apoptosis of cardiomyocytes.Hypoxic preconditioning significantly increased the expression of exosomal miR-486-5p,which directly targeted the phosphatase and tensin homolog.Additionally,hypo-Exos markedly activated the phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)pathway.Moreover,deletion of miR-486-5p in hypo-Exos counteracted the anti-apoptotic effects and suppressed PI3K/Akt pathway activation.CONCLUSION Hypoxic preconditioning augments anti-apoptotic properties of exosomes,primarily via miR-486-5p upregulation,which mediates its function by modulating the phosphatase and tensin homolog/PI3K/Akt axis.展开更多
This editorial highlights the randomized controlled trial by Qian et al evaluating the efficacy of nalbuphine in managing postoperative pain and associated inflammatory factors in patients undergoing radical gastrecto...This editorial highlights the randomized controlled trial by Qian et al evaluating the efficacy of nalbuphine in managing postoperative pain and associated inflammatory factors in patients undergoing radical gastrectomy for gastric cancer.Utilizing a multimodal analgesic approach with a controlled analgesia pump and a transverse abdominis muscle plane block,the study compared nalbuphine against sufentanil.Results indicate that nalbuphine significantly reduces pain scores at various postoperative intervals,decreases consumption and patientcontrolled analgesia presses,and lowers levels of pain markers such as prostaglandin E2,serotonin,and substance P.While both agents demonstrated effective pain control,nalbuphine was associated with a lower incidence of adverse effects,suggesting its potential as a valuable alternative in appropriate clinical scenarios.This study offers valuable insights into nalbuphine’s clinical applicability,underscoring its benefits for pain management and enhancing patient recovery in the postoperative setting.展开更多
Erratumto:Journal of Earth Science https://doi.org/10.1007/s12583-023-1946-6 The original version of this article unfortunately contained three mistakes.1.The presentation of Equation(4)was incorrect.The corrected one...Erratumto:Journal of Earth Science https://doi.org/10.1007/s12583-023-1946-6 The original version of this article unfortunately contained three mistakes.1.The presentation of Equation(4)was incorrect.The corrected one is given below.展开更多
Mounting evidence suggests that male sperm may be gradually depleted after consecutive matings,resulting in the decline of female reproductive output in insect species.It is predicted that females may employ adaptive ...Mounting evidence suggests that male sperm may be gradually depleted after consecutive matings,resulting in the decline of female reproductive output in insect species.It is predicted that females may employ adaptive strategies to avoid the sperm-depleted males,such as mating multiply with different males and/or discriminating against previously mated males(MMs).Similarly,males may exhibit adaptive behaviors toward females varying in mating status.However,in spiders with males lacking primary copulatory organs and their pedipalps modified to transfer sperm,there are few studies on male mating potential and previous mating experience on their subsequent mate choice.In this study,we used a polyandrous crab spider,Ebrechtella tricuspidata,a sit-and-wait predator with female-biased sex ratio as a model system to ascertain whether 1)male mating experiences influence female reproductive fitness;2)females respond differently to males varying in mating status;and 3)males respond differently to females varying in mating status.Our results showed that female fecundity was independent of male mating experiences,but female fertility markedly declined with the increase of male previous mating experiences in the first eggsac.Counter to our predictions,females preferred to choose and mate with the larger males,regardless of their mating status.In contrast,male mating status influenced their mating preference toward females.Virgin males did not show any preference between virgin and mated females;however,MMs were more likely to prefer the virgin females over the mated ones.Overall,our results indicated that female choice depended primarily on male relative size rather than male mating status,whereas male choice depended on female mating status,Depending on the investigation of reproductive dynamics and mate choice in E.tricuspidata,the study will provide valuable insights into the adaptive behaviors of both males and females exhibiting toward mates varying inmating status.展开更多
Polypropylene(PP) accounts for approximately 28.0% of the global polyolefin market,valued at $243.4 billion in 2022.Known for its lightweight,chemical resistance,costeffectiveness,high strength and melting point,PP is...Polypropylene(PP) accounts for approximately 28.0% of the global polyolefin market,valued at $243.4 billion in 2022.Known for its lightweight,chemical resistance,costeffectiveness,high strength and melting point,PP is widely used in various applications.Its properties and applications are closely tied to its tacticity.One-dimensional(1D) conventional 13C NMR has been extensively utilized to analyze PP tacticity,but its low sensitivity and longer relaxation time remain drawbacks.Typically,analyzing a single PP sample requires around 9 h of NMR time.Using of a cryogenically cooled 10 mm NMR probe can significantly reduce this measurement time;however,its high cost makes it inaccessible for most NMR laboratories.While the refocused insensitive nuclei enhanced by polarization transfer(RINEPT) technique is well-known for enhancing NMR sensitivity,there are no published studies using 1D 13C RINEPT to quantify PP tacticity.Relaxation agents like chromium(Ⅲ) acetylacetonate(Cr(acac)_(3)) have also been used to reduce relaxation times in polyolefin NMR analyses.Here we introduce a straightforward and easily implementable 1D 13C NMR method for rapid PP tacticity quantification.This method combines Cr(acac)_(3),Bruker's existing RINEPT pulse sequence(ineptrd),and our recently published ~1H decoupling sequence(bi_waltz65_256 pl) to eliminate ^(1)H decoupling artifacts.It is worth noting that decoupling artifacts are always present.When the signal-to-noise ratio(SNR) is low,these artifacts are obscured by noise.For example,in some two-dimensional(2D) or three-dimensional(3D) NMR spectra,decoupling artifacts are barely visible because of the low SNR.However,when attempting to observe weak signals in ^(1)D spectra,increasing the sample concentration or the number of scans enhances the SNR,revealing the decoupling artifacts.The decoupling artifacts appeared superimpose with some other weak signals,affecting the measurements of signal intensities.Therefore,improved ~1H-decoupling methods are crucial for such data acquisitio n.This synergy results in a 9.4-to 9.7-fold sensitivity enhancement,equating to an 88-to 94-fold reduction(9.4^(2)≈88,9.7^(2)≈94) in NMR acquisition time compared to conventional 1D ^(13)C NMR experiment with Cr(acac)_(3).The time savings are even more substantial compared to experiments without Cr(acac)_(3).The faster and quantitative approach is accessible to researchers with or without cryoprobes.Beyond PP,this method can be applied to tacticity measurements of other polyolefins,such as polybutene,polyhexene and polyoctene.展开更多
In response to the additional load impact caused by the integration of electric vehicles (EVs) into the grid or microgrids (MGs), as well as the issue of low responsiveness of EV users during vehicle-to-vehicle (V2V) ...In response to the additional load impact caused by the integration of electric vehicles (EVs) into the grid or microgrids (MGs), as well as the issue of low responsiveness of EV users during vehicle-to-vehicle (V2V) power exchange processes, this paper explores a multi-party energy trading model considering user responsiveness under low carbon goals. The model takes into account the stochastic charging and discharging characteristics of EVs, user satisfaction, and energy exchange costs, and formulates utility functions for participating entities. This transforms the competition in multi-party energy trading into a Bayesian game problem, which is subsequently resolved. Furthermore, this paper primarily employs sensitivity analysis to evaluate the impact of multi-party energy trading on user responsiveness and green energy utilization, with the aim of promoting incentives in the electricity trading market and aligning with low-carbon requirements. Finally, through case simulations, the effectiveness of this model for the considered scenarios is demonstrated.展开更多
With the rapid growth of the cosmetics industry,the demand for diversified and interdisciplinary talent is increasing.However,a structural mismatch persists between current educational approaches and industry needs,pa...With the rapid growth of the cosmetics industry,the demand for diversified and interdisciplinary talent is increasing.However,a structural mismatch persists between current educational approaches and industry needs,particularly in aligning with the holistic education model,which emphasizes moral,intellectual,physical,aesthetic,and labor development.To bridge this gap,Guangdong Pharmaceutical University has implemented a comprehensive reform of its holistic education system,aiming to cultivate well-rounded cosmetics professionals.Key initiatives include integrating professional ethics into moral education,strengthening the integration of theory and practice in intellectual training,emphasizing skin health management in physical education,enhancing aesthetic appreciation and creative thinking,and promoting labor spirit and practical skills through hands-on experiences.These reform efforts have yielded significant results,contributing high-quality talent to the cosmetics industry and offering a valuable model for other institutions seeking to align education with industry demands.展开更多
基金financially supported by the National Natural Science Foundation of China (52462032, 62274018, 52462031)Natural Science Foundation of Yunnan Province (202501AT070353, 202101BE070001-049)+2 种基金the Xinjiang Construction Corps Key Areas of Science and Technology Research Project (2023AB029)the Tianchi Talent Program of Xinjiang Uygur Autonomous Region (2024, Jiangzhao Chen)the Key Project of Chongqing Overseas Students Returning to China Entrepreneurship and Innovation Support Plan (cx2023006)。
文摘In recent years, the research advancements have high-lighted the critical role of the A-site cation in determining the optoelectronic and physicochemical properties of organicinorganic lead halide perovskites. Mixed-cation perovskites(MCPs) have been extensively used as absorber thin films in perovskite solar cells(PSCs), achieving high power conversion efficiencies(PCE) over 26%^([1, 2]).
基金supported by the National Natural Science Foundation of China(NSFC)under Grant No.22035001 and No.52233005.
文摘To achieve the target of carbon neutrality,it is crucial to develop an efficient and green synthesis methodology with good atomic economy to achieve sufficient utilization of energy and sustainable development.Photoinduced electron transfer reversible addition-fragmentation chain-transfer(PET-RAFT)polymerization is a precise methodology for constructing polymers with well-defined structures.However,conventional semiconductor-mediated PET-RAFT polymerization still has considerable limitations in terms of efficiency as well as the polymerization environment.Herein,sulfur-doped carbonized polymer dots(CPDs)were hydrothermally synthesized for catalysis of aqueous PET-RAFT polymerization at unprecedented efficiency with a highest propagation rate of 5.05 h-1.The resulting polymers have well-controlled molecular weight and narrow molecular weight dispersion(Ð<1.10).Based on the optoelectronic characterizations,we obtained insights into the photoinduced electron transfer process and proposed the mechanism for CPD-mediated PET-RAFT polymerization.In addition,as-synthesized CPDs for PET-RAFT polymerization were also demonstrated to be suitable for a wide range of light sources(blue/green/solar irradiation),numerous monomers,low catalyst loading(low as 0.01 mg mL^(-1)),and multiple polar solvent environments,all of which allowed to achieve efficiencies much higher than those of existing semiconductor-mediated methods.Finally,the CPDs were confirmed to be non-cytotoxic and catalyzed PET-RAFT polymerization successfully in cell culture media,indicating broad prospects in biomedical fields.
文摘<div style="text-align:justify;"> Dual polarization quadrature phase shift keying (DP-QPSK) is currently the main modulation format for high-speed optical fiber communications, and its simulation modeling has important implications for theoretical research and practical applications. Based on Optisystem 15 optical communication system simulation software, this paper builds a 100 Gbit/s rate DP-QPSK modulation format coherent optical transmission system simulation model, analyzes the working principles of the transmitting end, transmission link, and coherent receiver. The principle of signal recovery at the digital signal processing end is briefly explained. The simulation results show that the signal quality after DSP processing has been significantly improved. </div>
文摘Neural stem cells(NSCs)play a fundamental role in generating diverse neuronal populations that contribute to the formation of intricate neural circuitry.Disturbances arising from intrinsic or extrinsic factors can alter the developmental behavior of NSCs and disrupt nervous system homeostasis.While intrinsic regulatory mechanisms have been elucidated extensively in invertebrate or vertebrate models,the regulatory mechanisms underlying extrinsic cues from the cellular environment remain poorly understood.This review synthesized recent research on cellular ambient effects,including the microenvironment,systemic environment and external factors,on NSCs in Drosophila.Key topics include spatial cues,NSC-glia interactions,long-distance regulation by tissues such as the fat body,and the external environmental stressors like irradiation or viral infection.By integrating these findings,this review provides new insights into how extrinsic signals shape NSCs and bridges gaps between foundational research and clinical translation.
基金supported by the National Key R&D Program of China (No. 2023YFA1606401 and 2018YFA0404401)the Young Scholar of Regional Development,CAS ([2023] 15)+1 种基金Chinese Academy of Sciences Stable Support for Young Teams in Basic Research (No. YSBR-002)Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences (No. XDB34000000)
文摘Schottky mass spectrometry utilizing heavy-ion storage rings is a powerful technique for the precise mass and decay half-life measurements of highly charged ions.Owing to the nondestructive ion detection features of Schottky noise detectors,the number of stored ions in the ring is determined by the peak area in the measured revolution frequency spectrum.Because of their intrinsic amplitude-frequency characteristic(AFC),Schottky detector systems exhibit varying sensitivities at different frequencies.Using low-energy electron-cooled stored ions,a new method is developed to calibrate the AFC curve of the Schottky detector system of the Experimental Cooler Storage Ring(CSRe)storage ring located in Lanzhou,China.Using the amplitude-calibrated frequency spectrum,a notable refinement was observed in the precision of both the peak position and peak area.As a result,the storage lifetimes of the electron-cooled fully ionized^(56)Fe^(26+)ions were determined with high precision at beam energies of 13.7 and 116.4 MeV/u,despite of frequency drifts during the experiment.When electron cooling was turned off,the effective vacuum condition experienced by the 116.4 MeV/u^(56)Fe^(26+)ions was determined using amplitude-calibrated spectra,revealing a value of 2×10^(−10)mbar,which is consistent with vacuum gauge readings along the CSRe ring.The method reported herein will be adapted for the next-generation storage ring of the HIAF facility under construction in Huizhou,China.It can also be adapted to other storage ring facilities worldwide to improve precision and enhance lifetime measurements using many ions in the ring.
基金supported by the Science and Technology Programs of Department of Natural Resources of Zhejiang Province,China(No.2020006)the Zhejiang Provincial Natural Science Foundation of China(No.LGF22D030001)the Key R&D Program of Zhejiang Province,China(No.2021C04020)。
文摘Cadmium(Cd)contamination in soil can lead to food chain accumulation and greatly impacts on human health.Bioremediation has gained more and more attention due to its environment-friendly,high efficiency and low-cost.In this work,we studied the impact of phosphate solubilizing bacterial agent(PSBA)on Cd bioavailability,microbial communities in soil and Cd accumulation in lettuce plants with pot experiment and field trial.Results of pot experiment showed that PSBA could decrease the bioavailability of Cd(Cd-acid extractable from 3.30 to 2.34mg/kg,Cd-reducible from 1.94 to 1.56 mg/kg),promote lettuce plants growth(increased by 33.85%height and by 33.65%fresh weight)and reduce the accumulation of Cd(from 5.85 to 3.73 mg/kg)in lettuce plants.High-throughput sequencing identified that PSBA could change the composition and structure of the soil microbial communities.The relative abundances of the three ecologically beneficial bacterial families of Pseudomonadaceae,Burkholderiaceae,and Enterobacteriaceae increased from 2.29%to 5.13%,0.56%to 5.24%,and 1.87%to 16.93%,respectively.And the former two were positively correlated with redox potential(Eh)(R^(2)=0.474,p<0.05,R^(2)=0.590,p<0.01,respectively).The bacterial networks were more complex in PSBA treatment,reflecting through more links(from 1893 to 2185)and a higher average degree(from 38.242 to 45.052)and density(from 0.390 to 0.469).Results of field trial demonstrated that PSBA could also decrease Cd content in lettuce plants and microbial composition in soil.This study indicated that PSBA could be served as an alternative material in bioremediation of Cd contamination in soil.
基金the Precision Medical Center of Nanfang Hospital,with the informed consent of all participants and approved by the Medical Ethics Committee of the hospital(Approval No.NFEC-202110-K17-01).
文摘BACKGROUND Emerging evidence indicates that hypoxic preconditioning boosts the antioxidant and anti-apoptotic capacities of mesenchymal stem cell-derived exosomes;however,the specific mechanisms remain incompletely elucidated.This study explored the impact of hypoxia-preconditioned mesenchymal stem cell-derived exosomes(hypo-Exos)vs normoxic counterparts on the apoptotic response in cardiomyocytes triggered by oxidative stress.AIM To determine whether and how hypoxic preconditioning augments the cardioprotective efficacy of hypo-Exos against oxidative stress-induced cardiomyocyte apoptosis.METHODS H9C2 cardiomyocytes were treated with hydrogen peroxide(H2O2)to induce oxidative injury.Assessments of cell viability,oxidative biomarkers,and apoptotic activity were conducted to evaluate the therapeutic efficacy of hypo-Exos and normoxic counterparts.High-throughput sequencing was performed to identify potential target microRNAs(miRNAs).Luciferase reporter assays were conducted to confirm selected miRNAs binding to target genes.Hypo-Exos loaded with selected miRNAs antagomirs or negative controls were administered to H2O2-treated H9C2 cells to validate the downstream signaling pathways involved.RESULTS Hypo-Exos significantly enhanced cell viability,reduced oxidative stress,and inhibited apoptosis of cardiomyocytes.Hypoxic preconditioning significantly increased the expression of exosomal miR-486-5p,which directly targeted the phosphatase and tensin homolog.Additionally,hypo-Exos markedly activated the phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)pathway.Moreover,deletion of miR-486-5p in hypo-Exos counteracted the anti-apoptotic effects and suppressed PI3K/Akt pathway activation.CONCLUSION Hypoxic preconditioning augments anti-apoptotic properties of exosomes,primarily via miR-486-5p upregulation,which mediates its function by modulating the phosphatase and tensin homolog/PI3K/Akt axis.
基金Supported by National Natural Science Foundation of China,No.82404612.
文摘This editorial highlights the randomized controlled trial by Qian et al evaluating the efficacy of nalbuphine in managing postoperative pain and associated inflammatory factors in patients undergoing radical gastrectomy for gastric cancer.Utilizing a multimodal analgesic approach with a controlled analgesia pump and a transverse abdominis muscle plane block,the study compared nalbuphine against sufentanil.Results indicate that nalbuphine significantly reduces pain scores at various postoperative intervals,decreases consumption and patientcontrolled analgesia presses,and lowers levels of pain markers such as prostaglandin E2,serotonin,and substance P.While both agents demonstrated effective pain control,nalbuphine was associated with a lower incidence of adverse effects,suggesting its potential as a valuable alternative in appropriate clinical scenarios.This study offers valuable insights into nalbuphine’s clinical applicability,underscoring its benefits for pain management and enhancing patient recovery in the postoperative setting.
文摘Erratumto:Journal of Earth Science https://doi.org/10.1007/s12583-023-1946-6 The original version of this article unfortunately contained three mistakes.1.The presentation of Equation(4)was incorrect.The corrected one is given below.
基金the National Natural Science Foundation of China(30800121).
文摘Mounting evidence suggests that male sperm may be gradually depleted after consecutive matings,resulting in the decline of female reproductive output in insect species.It is predicted that females may employ adaptive strategies to avoid the sperm-depleted males,such as mating multiply with different males and/or discriminating against previously mated males(MMs).Similarly,males may exhibit adaptive behaviors toward females varying in mating status.However,in spiders with males lacking primary copulatory organs and their pedipalps modified to transfer sperm,there are few studies on male mating potential and previous mating experience on their subsequent mate choice.In this study,we used a polyandrous crab spider,Ebrechtella tricuspidata,a sit-and-wait predator with female-biased sex ratio as a model system to ascertain whether 1)male mating experiences influence female reproductive fitness;2)females respond differently to males varying in mating status;and 3)males respond differently to females varying in mating status.Our results showed that female fecundity was independent of male mating experiences,but female fertility markedly declined with the increase of male previous mating experiences in the first eggsac.Counter to our predictions,females preferred to choose and mate with the larger males,regardless of their mating status.In contrast,male mating status influenced their mating preference toward females.Virgin males did not show any preference between virgin and mated females;however,MMs were more likely to prefer the virgin females over the mated ones.Overall,our results indicated that female choice depended primarily on male relative size rather than male mating status,whereas male choice depended on female mating status,Depending on the investigation of reproductive dynamics and mate choice in E.tricuspidata,the study will provide valuable insights into the adaptive behaviors of both males and females exhibiting toward mates varying inmating status.
文摘Polypropylene(PP) accounts for approximately 28.0% of the global polyolefin market,valued at $243.4 billion in 2022.Known for its lightweight,chemical resistance,costeffectiveness,high strength and melting point,PP is widely used in various applications.Its properties and applications are closely tied to its tacticity.One-dimensional(1D) conventional 13C NMR has been extensively utilized to analyze PP tacticity,but its low sensitivity and longer relaxation time remain drawbacks.Typically,analyzing a single PP sample requires around 9 h of NMR time.Using of a cryogenically cooled 10 mm NMR probe can significantly reduce this measurement time;however,its high cost makes it inaccessible for most NMR laboratories.While the refocused insensitive nuclei enhanced by polarization transfer(RINEPT) technique is well-known for enhancing NMR sensitivity,there are no published studies using 1D 13C RINEPT to quantify PP tacticity.Relaxation agents like chromium(Ⅲ) acetylacetonate(Cr(acac)_(3)) have also been used to reduce relaxation times in polyolefin NMR analyses.Here we introduce a straightforward and easily implementable 1D 13C NMR method for rapid PP tacticity quantification.This method combines Cr(acac)_(3),Bruker's existing RINEPT pulse sequence(ineptrd),and our recently published ~1H decoupling sequence(bi_waltz65_256 pl) to eliminate ^(1)H decoupling artifacts.It is worth noting that decoupling artifacts are always present.When the signal-to-noise ratio(SNR) is low,these artifacts are obscured by noise.For example,in some two-dimensional(2D) or three-dimensional(3D) NMR spectra,decoupling artifacts are barely visible because of the low SNR.However,when attempting to observe weak signals in ^(1)D spectra,increasing the sample concentration or the number of scans enhances the SNR,revealing the decoupling artifacts.The decoupling artifacts appeared superimpose with some other weak signals,affecting the measurements of signal intensities.Therefore,improved ~1H-decoupling methods are crucial for such data acquisitio n.This synergy results in a 9.4-to 9.7-fold sensitivity enhancement,equating to an 88-to 94-fold reduction(9.4^(2)≈88,9.7^(2)≈94) in NMR acquisition time compared to conventional 1D ^(13)C NMR experiment with Cr(acac)_(3).The time savings are even more substantial compared to experiments without Cr(acac)_(3).The faster and quantitative approach is accessible to researchers with or without cryoprobes.Beyond PP,this method can be applied to tacticity measurements of other polyolefins,such as polybutene,polyhexene and polyoctene.
文摘In response to the additional load impact caused by the integration of electric vehicles (EVs) into the grid or microgrids (MGs), as well as the issue of low responsiveness of EV users during vehicle-to-vehicle (V2V) power exchange processes, this paper explores a multi-party energy trading model considering user responsiveness under low carbon goals. The model takes into account the stochastic charging and discharging characteristics of EVs, user satisfaction, and energy exchange costs, and formulates utility functions for participating entities. This transforms the competition in multi-party energy trading into a Bayesian game problem, which is subsequently resolved. Furthermore, this paper primarily employs sensitivity analysis to evaluate the impact of multi-party energy trading on user responsiveness and green energy utilization, with the aim of promoting incentives in the electricity trading market and aligning with low-carbon requirements. Finally, through case simulations, the effectiveness of this model for the considered scenarios is demonstrated.
基金Guangdong Provincial Graduate Education Innovation Program Project in 2024(Project No.:2024XSLT-039)。
文摘With the rapid growth of the cosmetics industry,the demand for diversified and interdisciplinary talent is increasing.However,a structural mismatch persists between current educational approaches and industry needs,particularly in aligning with the holistic education model,which emphasizes moral,intellectual,physical,aesthetic,and labor development.To bridge this gap,Guangdong Pharmaceutical University has implemented a comprehensive reform of its holistic education system,aiming to cultivate well-rounded cosmetics professionals.Key initiatives include integrating professional ethics into moral education,strengthening the integration of theory and practice in intellectual training,emphasizing skin health management in physical education,enhancing aesthetic appreciation and creative thinking,and promoting labor spirit and practical skills through hands-on experiences.These reform efforts have yielded significant results,contributing high-quality talent to the cosmetics industry and offering a valuable model for other institutions seeking to align education with industry demands.
