Quantitative phase imaging(QPI)enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes,revolutionizing label-free microscopy in cellular research.Differential phase ...Quantitative phase imaging(QPI)enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes,revolutionizing label-free microscopy in cellular research.Differential phase contrast(DPC),a non-interferometric QPI technique,requires only four intensity images under asymmetric illumination to recover the phase of a sample,offering the advantages of being label-free,non-coherent and highly robust.Its phase reconstruction result relies on precise modeling of the phase transfer function(PTF).However,in real optical systems,the PTF will deviate from its theoretical ideal due to the unknown wavefront aberrations,which will lead to significant artifacts and distortions in the reconstructed phase.We propose an aberration-corrected DPC(ACDPC)method that utilizes three intensity images under annular illumination to jointly retrieve the aberration and the phase,achieving high-quality QPI with minimal raw data.By employing three annular illuminations precisely matched to the numerical aperture of the objective lens,the object information is transmitted into the acquired intensity with a high signal-to-noise ratio.Phase retrieval is achieved by an iterative deconvolution algorithm that uses simulated annealing to estimate the aberration and further employs regularized deconvolution to reconstruct the phase,ultimately obtaining a refined complex pupil function and an aberration-corrected quantitative phase.We demonstrate that ACDPC is robust to multi-order aberrations without any priori knowledge,and can effectively retrieve and correct system aberrations to obtain high-quality quantitative phase.Experimental results show that ACDPC can clearly reproduce subcellular structures such as vesicles and lipid droplets with higher resolution than conventional DPC,which opens up new possibilities for more accurate subcellular structure analysis in cell biology.展开更多
Objective Great obstetrical syndrome(GOS)represents a group of pregnancy-related diseases that result in inadequate placentation.Most GOS cases end in preterm,either spontaneously or indicatively,and the use of antena...Objective Great obstetrical syndrome(GOS)represents a group of pregnancy-related diseases that result in inadequate placentation.Most GOS cases end in preterm,either spontaneously or indicatively,and the use of antenatal corticosteroids(ACS)is inevitably discussed.The placenta is an important,transient fetal-derived organ and is the embodiment of maternal or fetal well-being.However,few studies provide histological evidence of the placenta in GOS.This study aims to address these issues.Methods A total of 831 pregnant women were prospectively recruited.Placenta tissue was collected immediately and fixed with 4%paraformaldehyde solution for future H&E analysis.A novel checklist was devised to evaluate maternal vascular malperfusion sections on the basis of the commonly accepted Amsterdam placental workshop group consensus statement.Results A total of 131 patients were classified as having GOS.Comparisons between those with and without GOS revealed significant differences,including higher levels of distal villous hypoplasia,increased syncytial knots,accelerated villous maturation,and higher total scores in GOS.We found significant negative associations between GOS and neonatal weight,neonatal height,head circumference,placental surface area,placental volume,and placenta gross examination score.GOS neonates were 1.25 times more likely to have hyperbilirubinemia.Regarding the effect of ACS,a significant reduction in birthweight,height,and head circumference was observed,along with an increased risk of hyperbilirubinemia.Conclusion This study provides histological evidence of the GOS that supports the defective deep placentation hypothesis.Our research also contributes to benefit-risk consultation in the GOS,such as in cases of PE and FGR,where a balance between fetal lung maturation and short-term neonatal outcomes is crucial.展开更多
Objective Sleep is fundamental to the physical and mental health of both the general population and pregnant women.Most studies have focused on the impact of certain trimester sleep behaviors on gestational complicati...Objective Sleep is fundamental to the physical and mental health of both the general population and pregnant women.Most studies have focused on the impact of certain trimester sleep behaviors on gestational complications and birth outcomes.This study aimed to explore the association between maternal sleep duration and fetal growth development from as early as 23 gestational weeks to birth.Methods A total of 803 pregnant women were prospectively enrolled.The self-reported maternal nocturnal sleep duration during all 3 trimesters was recorded.The outcome measures were reference-population-based Z-scores of fetal biometric measurements obtained through routine ultrasonographic examination.Results Using multiple linear regression,a marginally significant negative association was observed between second-trimester sleep duration and second-trimester fetal head circumference(HC)and third-trimester fetal biparietal diameter(BPD).Then the associations of long sleep duration in each trimester with fetal biometry extreme values were evaluated.A significant impact of second-trimester long sleep duration on the second-trimester BPD below the 10th percentile of the reference population was observed.Longitudinal analysis reported similar results for BPD and HC.Conclusions Overall,a negative association between sleep duration and fetal biometric measurements was observed.Long sleep durations in the second trimester might negatively impact fetal growth,particularly brain parameters,including BPD and HC.展开更多
基金supported by the National Natural Science Foundation of China(62305162,62227818,62361136588)China Postdoctoral Science Foundation(2023TQ0160,2023M731683)+5 种基金Nanjing University of Science and Technology independent research project(30923010305)National Key Research and Development Program of China(2024YFE0101300)Biomedical Competition Foundation of Jiangsu Province(BE2022847)Key National Industrial Technology Cooperation Foundation of Jiangsu Province(BZ2022039)Fundamental Research Funds for the Central Universities(2023102001)Open Research Fund of Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense(JSGP202105,JSGP202201,JSGPCXZNGZ202401)。
文摘Quantitative phase imaging(QPI)enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes,revolutionizing label-free microscopy in cellular research.Differential phase contrast(DPC),a non-interferometric QPI technique,requires only four intensity images under asymmetric illumination to recover the phase of a sample,offering the advantages of being label-free,non-coherent and highly robust.Its phase reconstruction result relies on precise modeling of the phase transfer function(PTF).However,in real optical systems,the PTF will deviate from its theoretical ideal due to the unknown wavefront aberrations,which will lead to significant artifacts and distortions in the reconstructed phase.We propose an aberration-corrected DPC(ACDPC)method that utilizes three intensity images under annular illumination to jointly retrieve the aberration and the phase,achieving high-quality QPI with minimal raw data.By employing three annular illuminations precisely matched to the numerical aperture of the objective lens,the object information is transmitted into the acquired intensity with a high signal-to-noise ratio.Phase retrieval is achieved by an iterative deconvolution algorithm that uses simulated annealing to estimate the aberration and further employs regularized deconvolution to reconstruct the phase,ultimately obtaining a refined complex pupil function and an aberration-corrected quantitative phase.We demonstrate that ACDPC is robust to multi-order aberrations without any priori knowledge,and can effectively retrieve and correct system aberrations to obtain high-quality quantitative phase.Experimental results show that ACDPC can clearly reproduce subcellular structures such as vesicles and lipid droplets with higher resolution than conventional DPC,which opens up new possibilities for more accurate subcellular structure analysis in cell biology.
基金supported by the National Science Foundation of China(No.81873843)the National Science and Technology Pillar Program of China during the Twelfth Five-Year Plan Period(No.2014BA105B05)the Fundamental Research Funds for the Central Universities(No.2017 KFYXJJ102 and No.2019 KFYXKJC053).
文摘Objective Great obstetrical syndrome(GOS)represents a group of pregnancy-related diseases that result in inadequate placentation.Most GOS cases end in preterm,either spontaneously or indicatively,and the use of antenatal corticosteroids(ACS)is inevitably discussed.The placenta is an important,transient fetal-derived organ and is the embodiment of maternal or fetal well-being.However,few studies provide histological evidence of the placenta in GOS.This study aims to address these issues.Methods A total of 831 pregnant women were prospectively recruited.Placenta tissue was collected immediately and fixed with 4%paraformaldehyde solution for future H&E analysis.A novel checklist was devised to evaluate maternal vascular malperfusion sections on the basis of the commonly accepted Amsterdam placental workshop group consensus statement.Results A total of 131 patients were classified as having GOS.Comparisons between those with and without GOS revealed significant differences,including higher levels of distal villous hypoplasia,increased syncytial knots,accelerated villous maturation,and higher total scores in GOS.We found significant negative associations between GOS and neonatal weight,neonatal height,head circumference,placental surface area,placental volume,and placenta gross examination score.GOS neonates were 1.25 times more likely to have hyperbilirubinemia.Regarding the effect of ACS,a significant reduction in birthweight,height,and head circumference was observed,along with an increased risk of hyperbilirubinemia.Conclusion This study provides histological evidence of the GOS that supports the defective deep placentation hypothesis.Our research also contributes to benefit-risk consultation in the GOS,such as in cases of PE and FGR,where a balance between fetal lung maturation and short-term neonatal outcomes is crucial.
基金supported by the National Natural Science Foundation of China(No.81873843)the National Science and Technology Pillar Program of China during the Twelfth Five-Year Plan Period(No.2014BAI05B05)the Fundamental Research Funds for the Central Universities(No.2017KFYXJJ102 and 2019KFYXKJC053).
文摘Objective Sleep is fundamental to the physical and mental health of both the general population and pregnant women.Most studies have focused on the impact of certain trimester sleep behaviors on gestational complications and birth outcomes.This study aimed to explore the association between maternal sleep duration and fetal growth development from as early as 23 gestational weeks to birth.Methods A total of 803 pregnant women were prospectively enrolled.The self-reported maternal nocturnal sleep duration during all 3 trimesters was recorded.The outcome measures were reference-population-based Z-scores of fetal biometric measurements obtained through routine ultrasonographic examination.Results Using multiple linear regression,a marginally significant negative association was observed between second-trimester sleep duration and second-trimester fetal head circumference(HC)and third-trimester fetal biparietal diameter(BPD).Then the associations of long sleep duration in each trimester with fetal biometry extreme values were evaluated.A significant impact of second-trimester long sleep duration on the second-trimester BPD below the 10th percentile of the reference population was observed.Longitudinal analysis reported similar results for BPD and HC.Conclusions Overall,a negative association between sleep duration and fetal biometric measurements was observed.Long sleep durations in the second trimester might negatively impact fetal growth,particularly brain parameters,including BPD and HC.
