The lake hydrological and meteorological data of the Tibetan Plateau are not rich. This research reports the observed climatic data and measured water levels of saline lakes from the local meteorological stations in t...The lake hydrological and meteorological data of the Tibetan Plateau are not rich. This research reports the observed climatic data and measured water levels of saline lakes from the local meteorological stations in the Zabuye salt lake, the Dangqiong Co salt lake and the Bankog Co salt lake in recent two decades. Combining with satellite remote sensing maps, we have analyzed the changes of the water level of these three lakes in recent years and discussed the origins of the changes induced by the meteorological factors. The results show that the annual mean temperature and the water level reflect a general ascending trend in these three lakes during the observation period. The rising rates of the annual mean temperature were 0.08°C/yr during 1991-2014 and 0.07°C/yr during 2004-2014, and of the water level, were 0.032 m/yr and 0.24 m/yr, respectively. Analysis of changes of the meteorological factors shows the main cause for the increase of lake water quantity are the reduced lake evaporation and the increased precipitation in the lake basins by the rise of average temperature. Seasonal variation of lake water level is powered largely by the supply of lake water types and the seasonal change of regional climate.展开更多
In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still n...In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still needs to be further explored.Recent studies have found that the opening of the mitoKATP channel can regulate mitochondrial autophagy,and the initiation of miRNA-DNA methylation plays a regulatory role in inducing cell autophagy.The applicant research team previously found that Qishen Yiqi Dropping Pills could significantly improve myocardial ischemia by mediating MitokATP channels to regulate mitochondrial autophagy.,and animal experiments have confirmed that miR-155 plays a significant role in the aspect of autophagy regulates,inflammatory reaction and Vascular smooth muscle cell migration.Therefore,the applicant innovatively proposed that Qishen Yiqi Dropping Pills can regulate miRNA155-DNA methylation to mediate the opening of mitoKATP,thereby regulating mitochondrial autophagy and improving myocardial ischemia.In this paper,the association between mitochondrial autophagy and oxidative stress injury after myocardial ischemia was described,and the possible mechanism of Qisen Yiqi dropping pills regulating mitochondrial autophagy by regulating miRNA155-DNA methylation to mediate MitokATP to improve myocardial ischemia reperfusion injury was discussed,so as to provide theoretical ideas for related research.展开更多
Objective:To investigate the effects of Qishen Yiqi dropping pills serum on KATP channel opening and PI3K/AKT signaling pathway of hypoxic/reoxygenated H9C2 cardiocytes.Methods:H9C2 cardiocytes cultured in vitro were ...Objective:To investigate the effects of Qishen Yiqi dropping pills serum on KATP channel opening and PI3K/AKT signaling pathway of hypoxic/reoxygenated H9C2 cardiocytes.Methods:H9C2 cardiocytes cultured in vitro were randomly divided into five groups,A:H9C2 cell group B:H9C2 cells+H2O2 model group C:H9C2 cells+H2O2 model+Qishen Yiqi group D:H9C2 cells+H2O2 model+Qishen Yiqi+wort group E:H9C2 cells+H2O2 model+Qishenyiqi+5-HD group,the drug intervention is according to the corresponding conditions.CCK-8 method was used to detect the cell activity of each group;Western blot was used to detect the expression of AKT and P-Akt proteins in myocardial cells in each group.The current was recorded by the standard patch clamp whole cell recording method,and the current was collected and analyzed by Pclamp6.0 software.Results:CCK-8 test results showed that compared with group A,the activity of myocardial cells in group B was significantly decreased,and the difference was statistically significant(P<0.01);compared with group B,the difference in group C was statistically significant(P<0.01);compared with C,cardiomyocyte activity in D and E group were significantly decreased,and the difference was statistically significant(P<0.05);WB results showed that compared with A,p-Akt protein expression in B,C,D and E groups were significantly decreased,and the difference was statistically significant(P<0.01);compared with group B,p-Akt protein expression in C,D and E group were significantly increased,and the difference was statistically significant(P<0.01),but there was no significant difference in AKT expression among groups(P>0.05);The results of whole cell patch clamp experiment showed that the outward current of B was significantly increased compared with that of A,and the difference between groups was statistically significant(P<0.01);compared with group B,cardiomyocytes in group C further increased the outward current,and the difference between groups was statistically significant(P<0.01);compared with C,the current of D and E group were significantly decreased,with statistical significance between groups(P<0.01).Conclusion:QishenYiqi dropping pills can protect cardiomyocytes by activating p-Akt protein expression and KATP channel opening in H9C2 cardiomyocytes.展开更多
目的:如今机器学习算法逐渐被应用于预测脑卒中和心血管疾病方面。与传统回归模型相比,机器学习可以通过探索大量预测特征与结果变量之间的灵活关系,从数据中学习,以实现高预测准确性,为个体化治疗和康复方案的制定提供了新的方法。此...目的:如今机器学习算法逐渐被应用于预测脑卒中和心血管疾病方面。与传统回归模型相比,机器学习可以通过探索大量预测特征与结果变量之间的灵活关系,从数据中学习,以实现高预测准确性,为个体化治疗和康复方案的制定提供了新的方法。此文旨在系统评价基于机器学习脑卒中功能恢复及预后的预测模型,综合评估其预测性能及临床应用潜力,为相关预后预测模型的构建、应用及推广提供参考。方法:按照PRISMA指南进行系统评价。通过检索PubMed、EMbase、Web of Science核心数据库、中国知网、万方和中国生物医学文献数据库,筛选出使用机器学习方法进行脑卒中预后预测的相关文献,检索时限为2014-01-01/2024-07-01。由2名研究人员严格按照纳入与排除标准独立筛选文献、提取数据,使用预测模型偏倚风险评价工具评价模型质量。结果:①初步检索共获取3126篇文献,经过筛选和排除,最终纳入18篇研究,共运用13种机器学习方法构建了150个预测模型,其中应用次数最多的3种方法为逻辑回归、随机森林和极限梯度提升(XGBoost);仅有1项研究开展了外部验证;有8项研究报告了缺失数据的处理方法;②结局指标方面有8项研究采用了临床数据与影像学数据结合来构建模型,9项研究仅运用临床数据构建模型,1项研究仅用影像学数据构建模型;③18项研究均给出了研究中最重要的特征,其中被提及最多的是美国国立卫生研究院卒中量表和年龄;所有研究均报告了曲线下面积值,范围0.74-0.96,最高为0.96;所有模型的总体偏倚风险均为高偏倚风险,模型分析领域高偏倚风险是导致所有模型总体偏倚风险高的主要原因;④Meta分析结果显示年龄和美国国立卫生研究院卒中量表评分对脑卒中预后影响显著,年龄[MD=8.49,95%CI(6.24,10.75),P<0.01],美国国立卫生研究院卒中量表评分[MD=4.78,95%CI(2.56,7.00),P<0.01]。结论:此次研究系统评价了基于机器学习的脑卒中功能恢复及预后预测模型,模型均具有良好的预测潜力。但未来研究应增加纳入模型样本量,采用前瞻性研究,并且添加对模型的外部验证以提高模型的稳定性和预测准确性,控制偏倚风险,以帮助模型在实际临床应用中的验证和推广,同时应对缺失值的插补更透明和精准。虽然现有的机器学习模型显示出良好的预测性能,但也要注重模型的功能性和可用性,纳入特征多会降低易用性。应开发简便易用的模型接口和用户友好的临床工具,使医护人员能够更好地应用模型进行临床决策。展开更多
Objective:Adverse-risk acute myeloid leukemia(AML)patients should receive allogeneic hematopoietic stem cell transplantation(allo-HSCT)at first complete remission(CR1).However,the influence of prior therapies[i.e.,ven...Objective:Adverse-risk acute myeloid leukemia(AML)patients should receive allogeneic hematopoietic stem cell transplantation(allo-HSCT)at first complete remission(CR1).However,the influence of prior therapies[i.e.,venetoclax plus azacitidine(VEN-AZA)or intensive chemotherapy(IC)]on post-transplant outcomes remains inconclusive.This multicenter,retrospective study compared the post-transplant outcomes between patients receiving VEN-AZA and those receiving IC before allo-HSCT.Methods:This study was based on the transplant database of TROPHY group.Consecutive adverse-risk AML patients receiving allo-HSCT from January 2021 to June 2023 were screened in five Chinese transplant centers.Patients were categorized into VEN-AZA group if they received venetoclax combined with azacitidine as first-line therapy followed by allo-HSCT.Patients who received first-line therapy consisting of a mainstay treatment of cytarabine and anthracycline followed by allo-HSCT were categorized into IC group.Results:In the total cohort,the 3-year probabilities of overall survival,leukemia-free survival,and event-free survival were better in the IC group than VEN-AZA group,particularly for patients with ASXL1 mutations or SF3B1 mutations.However,the survival of the VEN-AZA group was not superior to that of IC group in patients aged≥55 years or those with the hematopoietic cell transplantation-comorbidity index scores≥1 before allo-HSCT.After propensity score matching(median age:VEN-AZA group:57 years;IC group:55 years),only the probability of overall survival for the IC group was better than that of VEN-AZA group(93.6%vs.78.0%,P=0.034)at the 1-year follow-up;however,all of the other clinical outcomes were comparable between the VEN-AZA and IC groups.The TP53 mutation was independently associated with post-transplant relapse and survival.Conclusions:Our results suggest that IC remains the cornerstone of therapy,whereas VEN-AZA may also be used in younger patients and medically fit patients with adverse-risk AML who are receiving allo-HSCT in CR1.展开更多
基金The Key Scientific Research Project of National Science and Technology Commission,No.K89-01-32National Natural Science Foundation of China,No.4907010123+1 种基金Special Scientific Research Projects in Public Welfare Profession of Ministry of Land and Resources of the People’s Republic of China,No.201011001Basic Research Fund,No.K1418
文摘The lake hydrological and meteorological data of the Tibetan Plateau are not rich. This research reports the observed climatic data and measured water levels of saline lakes from the local meteorological stations in the Zabuye salt lake, the Dangqiong Co salt lake and the Bankog Co salt lake in recent two decades. Combining with satellite remote sensing maps, we have analyzed the changes of the water level of these three lakes in recent years and discussed the origins of the changes induced by the meteorological factors. The results show that the annual mean temperature and the water level reflect a general ascending trend in these three lakes during the observation period. The rising rates of the annual mean temperature were 0.08°C/yr during 1991-2014 and 0.07°C/yr during 2004-2014, and of the water level, were 0.032 m/yr and 0.24 m/yr, respectively. Analysis of changes of the meteorological factors shows the main cause for the increase of lake water quantity are the reduced lake evaporation and the increased precipitation in the lake basins by the rise of average temperature. Seasonal variation of lake water level is powered largely by the supply of lake water types and the seasonal change of regional climate.
基金Regional Fund Project of National Natural Science Foundation of China(No.81460712)the Young and Middle-aged Teachers’Scientific Research Basic Ability Enhancement Project of Guangxi Universities(No.2020KY07026)Guangxi Graduate Education Innovation Program(No.YCXJ2021035)。
文摘In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium.However,the specific mechanism still needs to be further explored.Recent studies have found that the opening of the mitoKATP channel can regulate mitochondrial autophagy,and the initiation of miRNA-DNA methylation plays a regulatory role in inducing cell autophagy.The applicant research team previously found that Qishen Yiqi Dropping Pills could significantly improve myocardial ischemia by mediating MitokATP channels to regulate mitochondrial autophagy.,and animal experiments have confirmed that miR-155 plays a significant role in the aspect of autophagy regulates,inflammatory reaction and Vascular smooth muscle cell migration.Therefore,the applicant innovatively proposed that Qishen Yiqi Dropping Pills can regulate miRNA155-DNA methylation to mediate the opening of mitoKATP,thereby regulating mitochondrial autophagy and improving myocardial ischemia.In this paper,the association between mitochondrial autophagy and oxidative stress injury after myocardial ischemia was described,and the possible mechanism of Qisen Yiqi dropping pills regulating mitochondrial autophagy by regulating miRNA155-DNA methylation to mediate MitokATP to improve myocardial ischemia reperfusion injury was discussed,so as to provide theoretical ideas for related research.
基金Regional Fund Project of National Natural Science Foundation of China(No.81460712)the Young and Middle-aged Teachers’Scientific Research Basic Ability Enhancement Project of Guangxi Universities(No.2020KY07026)Guangxi Graduate Education Innovation Program(No.YCXJ2021035)。
文摘Objective:To investigate the effects of Qishen Yiqi dropping pills serum on KATP channel opening and PI3K/AKT signaling pathway of hypoxic/reoxygenated H9C2 cardiocytes.Methods:H9C2 cardiocytes cultured in vitro were randomly divided into five groups,A:H9C2 cell group B:H9C2 cells+H2O2 model group C:H9C2 cells+H2O2 model+Qishen Yiqi group D:H9C2 cells+H2O2 model+Qishen Yiqi+wort group E:H9C2 cells+H2O2 model+Qishenyiqi+5-HD group,the drug intervention is according to the corresponding conditions.CCK-8 method was used to detect the cell activity of each group;Western blot was used to detect the expression of AKT and P-Akt proteins in myocardial cells in each group.The current was recorded by the standard patch clamp whole cell recording method,and the current was collected and analyzed by Pclamp6.0 software.Results:CCK-8 test results showed that compared with group A,the activity of myocardial cells in group B was significantly decreased,and the difference was statistically significant(P<0.01);compared with group B,the difference in group C was statistically significant(P<0.01);compared with C,cardiomyocyte activity in D and E group were significantly decreased,and the difference was statistically significant(P<0.05);WB results showed that compared with A,p-Akt protein expression in B,C,D and E groups were significantly decreased,and the difference was statistically significant(P<0.01);compared with group B,p-Akt protein expression in C,D and E group were significantly increased,and the difference was statistically significant(P<0.01),but there was no significant difference in AKT expression among groups(P>0.05);The results of whole cell patch clamp experiment showed that the outward current of B was significantly increased compared with that of A,and the difference between groups was statistically significant(P<0.01);compared with group B,cardiomyocytes in group C further increased the outward current,and the difference between groups was statistically significant(P<0.01);compared with C,the current of D and E group were significantly decreased,with statistical significance between groups(P<0.01).Conclusion:QishenYiqi dropping pills can protect cardiomyocytes by activating p-Akt protein expression and KATP channel opening in H9C2 cardiomyocytes.
文摘目的:如今机器学习算法逐渐被应用于预测脑卒中和心血管疾病方面。与传统回归模型相比,机器学习可以通过探索大量预测特征与结果变量之间的灵活关系,从数据中学习,以实现高预测准确性,为个体化治疗和康复方案的制定提供了新的方法。此文旨在系统评价基于机器学习脑卒中功能恢复及预后的预测模型,综合评估其预测性能及临床应用潜力,为相关预后预测模型的构建、应用及推广提供参考。方法:按照PRISMA指南进行系统评价。通过检索PubMed、EMbase、Web of Science核心数据库、中国知网、万方和中国生物医学文献数据库,筛选出使用机器学习方法进行脑卒中预后预测的相关文献,检索时限为2014-01-01/2024-07-01。由2名研究人员严格按照纳入与排除标准独立筛选文献、提取数据,使用预测模型偏倚风险评价工具评价模型质量。结果:①初步检索共获取3126篇文献,经过筛选和排除,最终纳入18篇研究,共运用13种机器学习方法构建了150个预测模型,其中应用次数最多的3种方法为逻辑回归、随机森林和极限梯度提升(XGBoost);仅有1项研究开展了外部验证;有8项研究报告了缺失数据的处理方法;②结局指标方面有8项研究采用了临床数据与影像学数据结合来构建模型,9项研究仅运用临床数据构建模型,1项研究仅用影像学数据构建模型;③18项研究均给出了研究中最重要的特征,其中被提及最多的是美国国立卫生研究院卒中量表和年龄;所有研究均报告了曲线下面积值,范围0.74-0.96,最高为0.96;所有模型的总体偏倚风险均为高偏倚风险,模型分析领域高偏倚风险是导致所有模型总体偏倚风险高的主要原因;④Meta分析结果显示年龄和美国国立卫生研究院卒中量表评分对脑卒中预后影响显著,年龄[MD=8.49,95%CI(6.24,10.75),P<0.01],美国国立卫生研究院卒中量表评分[MD=4.78,95%CI(2.56,7.00),P<0.01]。结论:此次研究系统评价了基于机器学习的脑卒中功能恢复及预后预测模型,模型均具有良好的预测潜力。但未来研究应增加纳入模型样本量,采用前瞻性研究,并且添加对模型的外部验证以提高模型的稳定性和预测准确性,控制偏倚风险,以帮助模型在实际临床应用中的验证和推广,同时应对缺失值的插补更透明和精准。虽然现有的机器学习模型显示出良好的预测性能,但也要注重模型的功能性和可用性,纳入特征多会降低易用性。应开发简便易用的模型接口和用户友好的临床工具,使医护人员能够更好地应用模型进行临床决策。
基金supported by the Beijing Natural Science Foundation(No.Z230016)the National Key Research and Development Program of China(No.2022YFC 2502606)+4 种基金the Major Program of the National Natural Science Foundation of China(No.82293630)the Peking University Medicine Fund for the World’s Leading Discipline or Discipline Cluster Development(No.71003Y3035)the Plan Project of Tongzhou Municipal Science and Technology(No.KJ2024CX045)the National Natural Science Foundation of China(No.82170208)the Fundamental Research Funds for the Central Universities。
文摘Objective:Adverse-risk acute myeloid leukemia(AML)patients should receive allogeneic hematopoietic stem cell transplantation(allo-HSCT)at first complete remission(CR1).However,the influence of prior therapies[i.e.,venetoclax plus azacitidine(VEN-AZA)or intensive chemotherapy(IC)]on post-transplant outcomes remains inconclusive.This multicenter,retrospective study compared the post-transplant outcomes between patients receiving VEN-AZA and those receiving IC before allo-HSCT.Methods:This study was based on the transplant database of TROPHY group.Consecutive adverse-risk AML patients receiving allo-HSCT from January 2021 to June 2023 were screened in five Chinese transplant centers.Patients were categorized into VEN-AZA group if they received venetoclax combined with azacitidine as first-line therapy followed by allo-HSCT.Patients who received first-line therapy consisting of a mainstay treatment of cytarabine and anthracycline followed by allo-HSCT were categorized into IC group.Results:In the total cohort,the 3-year probabilities of overall survival,leukemia-free survival,and event-free survival were better in the IC group than VEN-AZA group,particularly for patients with ASXL1 mutations or SF3B1 mutations.However,the survival of the VEN-AZA group was not superior to that of IC group in patients aged≥55 years or those with the hematopoietic cell transplantation-comorbidity index scores≥1 before allo-HSCT.After propensity score matching(median age:VEN-AZA group:57 years;IC group:55 years),only the probability of overall survival for the IC group was better than that of VEN-AZA group(93.6%vs.78.0%,P=0.034)at the 1-year follow-up;however,all of the other clinical outcomes were comparable between the VEN-AZA and IC groups.The TP53 mutation was independently associated with post-transplant relapse and survival.Conclusions:Our results suggest that IC remains the cornerstone of therapy,whereas VEN-AZA may also be used in younger patients and medically fit patients with adverse-risk AML who are receiving allo-HSCT in CR1.
