Fibroblast activation protein(FAP)is overexpressed in cancer-associated fibroblasts across various cancer types.Numerous radiolabeled FAP inhibitors(FAPIs)(Fig.S1A)currently under clinical investigation have shown rem...Fibroblast activation protein(FAP)is overexpressed in cancer-associated fibroblasts across various cancer types.Numerous radiolabeled FAP inhibitors(FAPIs)(Fig.S1A)currently under clinical investigation have shown remarkable potential in cancer theranostics.展开更多
Background:The use of third-generation different tyrosine kinase inhibitors(TKIs)is considered the most effective option for treating advanced non-small cell lung cancer(aNSCLC)with epidermal growth factor receptor(EG...Background:The use of third-generation different tyrosine kinase inhibitors(TKIs)is considered the most effective option for treating advanced non-small cell lung cancer(aNSCLC)with epidermal growth factor receptor(EGFR)mutations.However,there is limited information on the efficacy and safety of aumolertinib in patients remains these cases.Methods:The clinical records of patients receiving aumolertinib as first-line therapy across four hospitals in the Guangxi Zhuang Autonomous Region from April 2020 to December 2021 were retrospectively analyzed,using progression-free survival(PFS)as the primary endpoint and overall survival(OS)representing the secondary endpoint.Adverse events(AEs)were assessed using the Common Terminology Criteria for Adverse Events(CTCAE v5.0).Results:Approximately 47 patients with EGFR-Mutant aNSCLC were recruited,including 1 squamous cell carcinoma(SCC)patient,1 EGFR G719C mutated patient,1 EGFR S768 patient mutated,and 1 EGFR KDD mutated patient.The average follow-up duration was 48.1 months concluding in August 2024.The median PFS(mPFS)was 22.2 months(95%CI 17.6 to 26.7),while the median OS(mOS)was 39.7 months(95%CI 32.6 to 46.9).Patients with deletion of exon19 in EGFR(19del)showeda mPFS of 28.4 months,markedlylonger than those with the L858R point mutation(L858R),who had a mPFS of 15.2 months(p<0.036).Overall,22 patients(46.8%)had central nervous system(CNS)metastases at the=basal level.The mPFS for this cohort was 19.7 months.Rashes(17.0%),skin decrustation(4.2%),pruritus(4.2%),dental ulcers(4.2%),increased creatine kinase(2.1%),and musculoskeletal pains(2.1%)were the most prevalent AEs in this study.Grade 3 and higher AEs were observed at a rate of 4.2%.Conclusion:This study concluded that aumolertinib has considerable safety and efficacy for EGFR-mutant NSCLC in a first-line defense.展开更多
The implementation of the ecological protection red line serves as a crucial protective barrier for the conservation of wild plants with extremely small populations by delineating specific geographical areas designate...The implementation of the ecological protection red line serves as a crucial protective barrier for the conservation of wild plants with extremely small populations by delineating specific geographical areas designated for their conservation.The implementation of a comprehensive set of conservation measures aimed at establishing a biodiversity conservation network for wild plants with extremely small populations has laid the foundation for the health and stability of ecosystems,as well as the harmonious coexistence of humans and the natural environment.Research on the conservation of wild plants with extremely small populations serves as a critical foundation for informing and enhancing the effective conservation of such populations.This paper provides a systematic examination of the theoretical and practical conservation status of certain wild plants with extremely small populations and discusses the conservation trends of these populations within the framework of ecological protection red line delineation.In the future,strategies for the conservation of wild plants with extremely small populations should be integrated with an analysis of the causes of endangerment.This approach will provide a robust foundation for applied research focused on the conservation of these vulnerable plant populations.展开更多
Two^(87)Sr optical lattice clocks(OLCs)are being developed and operated at the National Institute of Metrology(NIM),located on different campuses that are 40km apart.In order to compare the optical frequencies of thes...Two^(87)Sr optical lattice clocks(OLCs)are being developed and operated at the National Institute of Metrology(NIM),located on different campuses that are 40km apart.In order to compare the optical frequencies of these two Sr OLCs,a 58-km noise canceled fiber link is built to transfer both a 1542-nm transfer laser and a microwave reference from Changping campus to Hepingli campus.Two commercial optical frequency combs(OFCs)with adapted single-branch 698/1542nm outputs coherently unite the two 698-nm clock lasers and the 1542-nm transfer laser.The fractional instability of the comparison yields 3.1×10^(−17)at 10000 s averaging time.The measured fractional frequency difference between these two Sr OLCs was evaluated to be 1.9(3.2)×10^(−17),which is within their claimed uncertainties.This result demonstrates the consistency of their frequencies when they serve as optical frequency standards.Our remote comparison demonstrates the feasibility of optical clock comparison through a long-distance fiber link and contributes to the progress of redefinition of the SI second.展开更多
Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this ...Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this paper,we introduce the first Chinese version of the PAQ and examine its psychometric properties and clinical applications.Methods In Study 1,the PAQ was administered to 990 Chinese participants.We examined its factor structure,internal consistency,test-retest reliability,as well as convergent,concurrent and discriminant validity.In Study 2,four groups,including a major depressive disorder(MDD)group(n=50),a matched healthy control group for MDD(n=50),a subclinical depression group(n=50)and a matched healthy control group for subclinical depression(n=50),were recruited.Group comparisons were conducted to assess the clinical relevance of the PAQ.Results In Study 1,the intended five-factor structure of the PAQ was found to fit the data well.The PAQ showed good internal consistency and test-retest reliability,as well as good convergent,concurrent and discriminant validity.In Study 2,the PAQ was able to successfully distinguish the MDD group and the subclinical depression group from their matched healthy controls.Conclusions The Chinese version of the PAQ is a valid and reliable instrument for comprehensively assessing alexithymia in the general population and adults with clinical/subclinical depression.展开更多
Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a...Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.展开更多
Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is...Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is typically accompanied by a poor prognosis.Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy.Methods:This study investigated whether 3-Methyladenine(3-MA)could potentiated the effect of PTX and its potential molecular mechanism.Samples were divided into the following categories:Negative control(NC)with the solvent dimethyl sulfoxide(DMSO,0.5%v/v),PTX(400 nM),3-MA(4 mM),and PTX(400 nM)+3-MA(4 mM).The viability of NPC cells was assessed using both the cell counting kit-8(CCK-8)assay and the colony formation assay.Microscopic observation was performed to identify morphological cell changes.Flow cytometry was used to assess cell cycle status,mitochondrial membrane potential(MMP),and apoptotic cells.Western blotting was conducted to quantify the protein expression.Results:3-MA enhanced PTX-specific inhibition of NPC cell proliferation.PTX,either alone or in combination with 3-MA,caused cell cycle halt at the G2/M phase in the majority of NPC cells,and the combination treatment of PTX with 3-MA induced a higher rate of NPC cell death compared to PTX alone.Western blotting results revealed the combination of PTX with 3-MA heightened activation of cyclin-dependent kinase 1(CDK1),a key molecule in shifting cells from mitotic arrest to apoptosis,led to a reduction in Myeloid Cell Leukemia 1(MCL-1)expression and an increase in Poly(ADP-ribose)polymerase(PARP)cleavage.Conclusion:The concurrent administration of PTX with 3-MA effectively enhances PTX’s inhibitory impact on NPC and activates the apoptosis signal regulated by CDK1.展开更多
<div style="text-align:justify;"> Precipitation is an important part of the global hydrological cycle. The large- scale, high-precision continuous precipitation data obtained by satellite remote sensin...<div style="text-align:justify;"> Precipitation is an important part of the global hydrological cycle. The large- scale, high-precision continuous precipitation data obtained by satellite remote sensing detection technology has become an important source of spatial precipitation data. However, because the spatial resolution of remote sensing precipitation data is still low, it is difficult to meet the needs of hydrological research, which restricts their application in drought and flood analysis, hydrological simulation, etc. In response to this problem, this paper takes the Beijing-Tianjin-Hebei region as the research area, downscaling the TRMM data and the GPM data space of the continuation plan, and increasing the spatial resolution of the data to 1 km. Compared with the original data, spatial downscaling data not only greatly improves the spatial resolution, but also increases the accuracy of the data, which has better applicability. </div>展开更多
Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic ...Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain undetermined.With innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental models.Differentially expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic stroke.Moreover,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.In particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic stroke.In this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.Findings from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.展开更多
Fluoride-based electrolyte exhibits extraordinarily high oxidative stability in high-voltage lithium metal batteries(h-LMBs) due to the inherent low highest occupied molecular orbital(HOMO) of fiuorinated solvents. Ho...Fluoride-based electrolyte exhibits extraordinarily high oxidative stability in high-voltage lithium metal batteries(h-LMBs) due to the inherent low highest occupied molecular orbital(HOMO) of fiuorinated solvents. However, such fascinating properties do not bring long-term cyclability of h-LMBs. One of critical challenges is the interface instability in contacting with the Li metal anode, as fiuorinated solvents are highly susceptible to exceptionally reductive metallic Li attributed to its low lowest unoccupied molecular orbital(LUMO), which leads to significant consumption of the fiuorinated components upon cycling.Herein, attenuating reductive decomposition of fiuorinated electrolytes is proposed to circumvent rapid electrolyte consumption. Specifically, the vinylene carbonate(VC) is selected to tame the reduction decomposition by preferentially forming protective layer on the Li anode. This work, experimentally and computationally, demonstrates the importance of pre-passivation of Li metal anodes at high voltage to attenuate the decomposition of fiuoroethylene carbonate(FEC). It is expected to enrich the understanding of how VC attenuate the reactivity of FEC, thereby extending the cycle life of fiuorinated electrolytes in high-voltage Li-metal batteries.展开更多
The NASICON-structured Na_(2)VTi(PO_(4))_(3)(NVTPO)has attracted significant attention due to its exceptional structural stability and rapid Na~+mobility.However,the development of this material has been hindered by p...The NASICON-structured Na_(2)VTi(PO_(4))_(3)(NVTPO)has attracted significant attention due to its exceptional structural stability and rapid Na~+mobility.However,the development of this material has been hindered by poor electronic conductivity and inadequate low-temperature performance.Herein,a feasible strategy of lattice regulation integrated with surface modification for NVTPO by nitrogen(N)deep doping is proposed.Systematic characterizations and theoretical calculations confirm that N is doped into both the inner crystal structure of NVTPO and the outer carbon layer.The blueshift of the P—O bonds and charge redistribution induced by the V/Ti—N bonds strengthen the local environment and narrow the bandgap,thereby enabling reversible structural evolution and improving electronic conductivity.As expected,the optimized NVTPO/N@CN material achieves an ultra-high capacity of 188.48 mA h g^(-1)at 10 mA g^(-1)and a long-term lifespan of 2000 cycles at 1 A g^(-1).More importantly,it exhibits competitive low-temperature performance(92.15%retention after 1000 cycles at 300 mA g^(-1)and-15℃)due to reduced charge transfer impedance and activation energy.This deep doping strategy modification is expected to broaden the applications of NASICON-type cathodes.展开更多
The effect from the interaction of the alternating current(AC)magnetic field with kilogram-level test mass(TM)limits the detectivity of the TianQin space-based gravitational wave detection.The quantifed effect require...The effect from the interaction of the alternating current(AC)magnetic field with kilogram-level test mass(TM)limits the detectivity of the TianQin space-based gravitational wave detection.The quantifed effect requires the determination of the AC magnetic susceptibilityχ(f)of the TM.A torque method is proposed to measure theχ(f)of kg-level samples at the mHz band with a precision of 1×10^(-7).Combined with our previous work[Phys.Rev.Appl.18044010(2022)],the general frequency-dependent susceptibility of the alloy cube with side length L and electrical conductivityσis determined asχ(f)=χ0+(0.24±0.01)σμ0L^(2)f from 0.1 mHz to 1 Hz.The determination is helpful for the preliminary estimation of the in-band eddy current efect in the TianQin noise budget.The technique can be adopted to accurately measureχ(f)of the actual TM in other precision experiments,where the magnetic noise is a signifcant detection limit.展开更多
Human herpesvirus,a specific group within the herpesvirus family,is responsible for a variety of human diseases.These viruses can infect humans and other vertebrates,primarily targeting the skin,mucous membranes,and n...Human herpesvirus,a specific group within the herpesvirus family,is responsible for a variety of human diseases.These viruses can infect humans and other vertebrates,primarily targeting the skin,mucous membranes,and neural tissues,thereby signifi-cantly impacting the health of both humans and animals.Animal models are crucial for studying virus pathogenesis,vaccine development,and drug testing.Despite several vaccine candidates being in preclinical and clinical stages,no vaccines are current available to prevent lifelong infections caused by these human herpesviruses,except for varicella-zoster virus(VZV)vaccine.However,the strict host tropism of herpes-viruses and other limitations mean that no single animal model can fully replicate all key features of human herpesvirus-associated diseases.This makes it challeng-ing to evaluate vaccines and antivirals against human herpesvirus comprehensively.Herein,we summarize the current animal models used to study the human herpesvi-ruses includingα-herpesviruses(herpes simplex virus type 1(HSV-1),HSV-2,VZV),β-herpesviruses(human cytomegalovirus(HCMV),γ-herpesviruses(Epstein-Barr virus(EBV))and Kaposi's sarcoma herpesvirus(KSHV)).By providing concise information and detailed analysis of the potential,limitations and applications of various models,such as non-human primates,mice,rabbits,guinea pigs,and tree shrews,this sum-mary aims to help researchers efficiently select the most appropriate animal model,offering practical guidance for studying human herpesvirus.展开更多
基金supported by the grants provided by Zhuhai People's Hospital,China(Grant No.:2021KYQD-03)the National Natural Science Foundation of China(Grant No.:22176016).
文摘Fibroblast activation protein(FAP)is overexpressed in cancer-associated fibroblasts across various cancer types.Numerous radiolabeled FAP inhibitors(FAPIs)(Fig.S1A)currently under clinical investigation have shown remarkable potential in cancer theranostics.
基金supported by the National Natural Science Foundation of China(grant nos.82260498 and 82160471)Quzhou City Qujiang District Life Oasis PublicWelfare Service Center,Health Development Promotion Project-Cancer Research Project(grant no.BJHA-CRP-033)+1 种基金Guangxi Medical and Health Key Discipline Construction Project,2021 Guilin City Science Research and Technology Development Plan Project(grant no.20210227-7-9)Beijing Xisike Clinical Oncology Research Foundation(grant no.Y-QL202101-0214).
文摘Background:The use of third-generation different tyrosine kinase inhibitors(TKIs)is considered the most effective option for treating advanced non-small cell lung cancer(aNSCLC)with epidermal growth factor receptor(EGFR)mutations.However,there is limited information on the efficacy and safety of aumolertinib in patients remains these cases.Methods:The clinical records of patients receiving aumolertinib as first-line therapy across four hospitals in the Guangxi Zhuang Autonomous Region from April 2020 to December 2021 were retrospectively analyzed,using progression-free survival(PFS)as the primary endpoint and overall survival(OS)representing the secondary endpoint.Adverse events(AEs)were assessed using the Common Terminology Criteria for Adverse Events(CTCAE v5.0).Results:Approximately 47 patients with EGFR-Mutant aNSCLC were recruited,including 1 squamous cell carcinoma(SCC)patient,1 EGFR G719C mutated patient,1 EGFR S768 patient mutated,and 1 EGFR KDD mutated patient.The average follow-up duration was 48.1 months concluding in August 2024.The median PFS(mPFS)was 22.2 months(95%CI 17.6 to 26.7),while the median OS(mOS)was 39.7 months(95%CI 32.6 to 46.9).Patients with deletion of exon19 in EGFR(19del)showeda mPFS of 28.4 months,markedlylonger than those with the L858R point mutation(L858R),who had a mPFS of 15.2 months(p<0.036).Overall,22 patients(46.8%)had central nervous system(CNS)metastases at the=basal level.The mPFS for this cohort was 19.7 months.Rashes(17.0%),skin decrustation(4.2%),pruritus(4.2%),dental ulcers(4.2%),increased creatine kinase(2.1%),and musculoskeletal pains(2.1%)were the most prevalent AEs in this study.Grade 3 and higher AEs were observed at a rate of 4.2%.Conclusion:This study concluded that aumolertinib has considerable safety and efficacy for EGFR-mutant NSCLC in a first-line defense.
基金Supported by 2024 Central Financial Comprehensive Protection Project for Abies chensiensis(610000242000000026647)2024 Provincial Forestry and Grassland Reform and Development Fund for the National Key Wild Plant Protection Project(610000242000000028574)2024 Biodiversity Conservation Project(610000242000000024911).
文摘The implementation of the ecological protection red line serves as a crucial protective barrier for the conservation of wild plants with extremely small populations by delineating specific geographical areas designated for their conservation.The implementation of a comprehensive set of conservation measures aimed at establishing a biodiversity conservation network for wild plants with extremely small populations has laid the foundation for the health and stability of ecosystems,as well as the harmonious coexistence of humans and the natural environment.Research on the conservation of wild plants with extremely small populations serves as a critical foundation for informing and enhancing the effective conservation of such populations.This paper provides a systematic examination of the theoretical and practical conservation status of certain wild plants with extremely small populations and discusses the conservation trends of these populations within the framework of ecological protection red line delineation.In the future,strategies for the conservation of wild plants with extremely small populations should be integrated with an analysis of the causes of endangerment.This approach will provide a robust foundation for applied research focused on the conservation of these vulnerable plant populations.
基金supported by the National Key R&D Program of China(Grant No.2021YFF0603802)the National Natural Science Foundation of China(Grant No.61905231)+1 种基金the State Administration for Market Regulation(Grant No.CXTD202301)the Innovation Program for Quantum Science and Technology(Grant No.2021ZD0300902)。
文摘Two^(87)Sr optical lattice clocks(OLCs)are being developed and operated at the National Institute of Metrology(NIM),located on different campuses that are 40km apart.In order to compare the optical frequencies of these two Sr OLCs,a 58-km noise canceled fiber link is built to transfer both a 1542-nm transfer laser and a microwave reference from Changping campus to Hepingli campus.Two commercial optical frequency combs(OFCs)with adapted single-branch 698/1542nm outputs coherently unite the two 698-nm clock lasers and the 1542-nm transfer laser.The fractional instability of the comparison yields 3.1×10^(−17)at 10000 s averaging time.The measured fractional frequency difference between these two Sr OLCs was evaluated to be 1.9(3.2)×10^(−17),which is within their claimed uncertainties.This result demonstrates the consistency of their frequencies when they serve as optical frequency standards.Our remote comparison demonstrates the feasibility of optical clock comparison through a long-distance fiber link and contributes to the progress of redefinition of the SI second.
基金This work was supported by a grant from the National Natural Science Foundation of China(32200906)STI 2030-Major Projects(2021ZD0201705)+2 种基金Major Project of Philosophy and Social Science Research of the Ministry of Education of China(22JZD044)Research Project of Shanghai Science and Technology Commission(20dz2260300)Fundamental Research Funds for the Central Universities and Starting Research Fund from Hangzhou Normal University.
文摘Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this paper,we introduce the first Chinese version of the PAQ and examine its psychometric properties and clinical applications.Methods In Study 1,the PAQ was administered to 990 Chinese participants.We examined its factor structure,internal consistency,test-retest reliability,as well as convergent,concurrent and discriminant validity.In Study 2,four groups,including a major depressive disorder(MDD)group(n=50),a matched healthy control group for MDD(n=50),a subclinical depression group(n=50)and a matched healthy control group for subclinical depression(n=50),were recruited.Group comparisons were conducted to assess the clinical relevance of the PAQ.Results In Study 1,the intended five-factor structure of the PAQ was found to fit the data well.The PAQ showed good internal consistency and test-retest reliability,as well as good convergent,concurrent and discriminant validity.In Study 2,the PAQ was able to successfully distinguish the MDD group and the subclinical depression group from their matched healthy controls.Conclusions The Chinese version of the PAQ is a valid and reliable instrument for comprehensively assessing alexithymia in the general population and adults with clinical/subclinical depression.
基金supported by Heilongjiang Provincial Natural Science Foundation of China(Grant No.YQ2022C012)China Postdoctoral Science Foundation(Grant No.2022MD713728)+1 种基金Heilongjiang Provincial Postdoctoral Fund(Grant No.LBHZ21046)the Open Project of Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,and National Key Research and Development Program of China(Grant No.2023YFD1201501).
文摘Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.
基金supported by the Science and Technology Innovation Program of Hunan Province(Grant Numbers:2021SK1014 and 2022WZ1027)the Colleges and Universities of Hunan Province(Grant Number:HNJG 20200440)+1 种基金the Scientific Research Fund of Hunan Provincial Education Department(Grant Number:21B0411)the Scientific Research Project of Changsha Central Hospital(Number:YNKY202201).
文摘Background:Nasopharyngeal carcinoma(NPC)exhibits a significant prevalence in the southern regions of China,and paclitaxel(PTX)is frequently employed as a medication for managing advanced NPC.However,drug resistance is typically accompanied by a poor prognosis.Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy.Methods:This study investigated whether 3-Methyladenine(3-MA)could potentiated the effect of PTX and its potential molecular mechanism.Samples were divided into the following categories:Negative control(NC)with the solvent dimethyl sulfoxide(DMSO,0.5%v/v),PTX(400 nM),3-MA(4 mM),and PTX(400 nM)+3-MA(4 mM).The viability of NPC cells was assessed using both the cell counting kit-8(CCK-8)assay and the colony formation assay.Microscopic observation was performed to identify morphological cell changes.Flow cytometry was used to assess cell cycle status,mitochondrial membrane potential(MMP),and apoptotic cells.Western blotting was conducted to quantify the protein expression.Results:3-MA enhanced PTX-specific inhibition of NPC cell proliferation.PTX,either alone or in combination with 3-MA,caused cell cycle halt at the G2/M phase in the majority of NPC cells,and the combination treatment of PTX with 3-MA induced a higher rate of NPC cell death compared to PTX alone.Western blotting results revealed the combination of PTX with 3-MA heightened activation of cyclin-dependent kinase 1(CDK1),a key molecule in shifting cells from mitotic arrest to apoptosis,led to a reduction in Myeloid Cell Leukemia 1(MCL-1)expression and an increase in Poly(ADP-ribose)polymerase(PARP)cleavage.Conclusion:The concurrent administration of PTX with 3-MA effectively enhances PTX’s inhibitory impact on NPC and activates the apoptosis signal regulated by CDK1.
文摘<div style="text-align:justify;"> Precipitation is an important part of the global hydrological cycle. The large- scale, high-precision continuous precipitation data obtained by satellite remote sensing detection technology has become an important source of spatial precipitation data. However, because the spatial resolution of remote sensing precipitation data is still low, it is difficult to meet the needs of hydrological research, which restricts their application in drought and flood analysis, hydrological simulation, etc. In response to this problem, this paper takes the Beijing-Tianjin-Hebei region as the research area, downscaling the TRMM data and the GPM data space of the continuation plan, and increasing the spatial resolution of the data to 1 km. Compared with the original data, spatial downscaling data not only greatly improves the spatial resolution, but also increases the accuracy of the data, which has better applicability. </div>
基金supported by the National Natural Science Foundation of China,Nos.82301486(to SL)and 82071325(to FY)Medjaden Academy&Research Foundation for Young Scientists,No.MJR202310040(to SL)+2 种基金Nanjing Medical University Science and Technique Development,No.NMUB20220060(to SL)Medical Scientific Research Project of Jiangsu Commission of Health,No.ZDA2020019(to JZ)Health China Buchang Zhiyuan Public Welfare Project for Heart and Brain Health,No.HIGHER202102(to QD).
文摘Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and disability.Accurate predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain undetermined.With innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental models.Differentially expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic stroke.Moreover,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.In particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic stroke.In this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and prognosis.Findings from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.
基金supported by the National Natural Science Foundation of China (Nos. 22379121, 62005216)Basic Public Welfare Research Program of Zhejiang (No. LQ22F050013)+1 种基金Zhejiang Province Key Laboratory of Flexible Electronics Open Fund (2023FE005)Shenzhen Foundation Research Program (No. JCYJ20220530112812028)。
文摘Fluoride-based electrolyte exhibits extraordinarily high oxidative stability in high-voltage lithium metal batteries(h-LMBs) due to the inherent low highest occupied molecular orbital(HOMO) of fiuorinated solvents. However, such fascinating properties do not bring long-term cyclability of h-LMBs. One of critical challenges is the interface instability in contacting with the Li metal anode, as fiuorinated solvents are highly susceptible to exceptionally reductive metallic Li attributed to its low lowest unoccupied molecular orbital(LUMO), which leads to significant consumption of the fiuorinated components upon cycling.Herein, attenuating reductive decomposition of fiuorinated electrolytes is proposed to circumvent rapid electrolyte consumption. Specifically, the vinylene carbonate(VC) is selected to tame the reduction decomposition by preferentially forming protective layer on the Li anode. This work, experimentally and computationally, demonstrates the importance of pre-passivation of Li metal anodes at high voltage to attenuate the decomposition of fiuoroethylene carbonate(FEC). It is expected to enrich the understanding of how VC attenuate the reactivity of FEC, thereby extending the cycle life of fiuorinated electrolytes in high-voltage Li-metal batteries.
基金supported by the National Natural Science Foundation of China(22272205 and 22178094)the Hunan Provincial Nature Science Foundation of China(2022JJ30685,2023JJ20059 and 2024JJ5292)+2 种基金the Hunan Provincial Science and Technology Plan Projects of China(2022RC3050 and 2017TP1001)the financial support from the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Provincesupport from the Young Elite Scientists Sponsorship Program by CAST(YESS20220432)。
文摘The NASICON-structured Na_(2)VTi(PO_(4))_(3)(NVTPO)has attracted significant attention due to its exceptional structural stability and rapid Na~+mobility.However,the development of this material has been hindered by poor electronic conductivity and inadequate low-temperature performance.Herein,a feasible strategy of lattice regulation integrated with surface modification for NVTPO by nitrogen(N)deep doping is proposed.Systematic characterizations and theoretical calculations confirm that N is doped into both the inner crystal structure of NVTPO and the outer carbon layer.The blueshift of the P—O bonds and charge redistribution induced by the V/Ti—N bonds strengthen the local environment and narrow the bandgap,thereby enabling reversible structural evolution and improving electronic conductivity.As expected,the optimized NVTPO/N@CN material achieves an ultra-high capacity of 188.48 mA h g^(-1)at 10 mA g^(-1)and a long-term lifespan of 2000 cycles at 1 A g^(-1).More importantly,it exhibits competitive low-temperature performance(92.15%retention after 1000 cycles at 300 mA g^(-1)and-15℃)due to reduced charge transfer impedance and activation energy.This deep doping strategy modification is expected to broaden the applications of NASICON-type cathodes.
基金supported by the National Key R&D Program of China(Grant No.2020YFC2200500)the Key Laboratory of Tian Qin Project(Sun Yat-sen University),Ministry of Education+1 种基金the National Natural Science Foundation of China(Grant Nos.12075325,12005308,and 11605065)the Doctoral Research Foundation Project of Hubei University of Arts and Science(Grant No.kyqdf2059017)。
文摘The effect from the interaction of the alternating current(AC)magnetic field with kilogram-level test mass(TM)limits the detectivity of the TianQin space-based gravitational wave detection.The quantifed effect requires the determination of the AC magnetic susceptibilityχ(f)of the TM.A torque method is proposed to measure theχ(f)of kg-level samples at the mHz band with a precision of 1×10^(-7).Combined with our previous work[Phys.Rev.Appl.18044010(2022)],the general frequency-dependent susceptibility of the alloy cube with side length L and electrical conductivityσis determined asχ(f)=χ0+(0.24±0.01)σμ0L^(2)f from 0.1 mHz to 1 Hz.The determination is helpful for the preliminary estimation of the in-band eddy current efect in the TianQin noise budget.The technique can be adopted to accurately measureχ(f)of the actual TM in other precision experiments,where the magnetic noise is a signifcant detection limit.
基金National Natural Science Foundation of China,Grant/Award Number:82222041 and 82241068CAMS Innovation Fund for Medical Sciences,Grant/Award Number:2021-I2M-1-037 and 2023-I2M-2-001+1 种基金National Key Research and Development Project of China,Grant/Award Number:2023YFC2309000Beijing Natural Science Foundation,Grant/Award Number:Z220018。
文摘Human herpesvirus,a specific group within the herpesvirus family,is responsible for a variety of human diseases.These viruses can infect humans and other vertebrates,primarily targeting the skin,mucous membranes,and neural tissues,thereby signifi-cantly impacting the health of both humans and animals.Animal models are crucial for studying virus pathogenesis,vaccine development,and drug testing.Despite several vaccine candidates being in preclinical and clinical stages,no vaccines are current available to prevent lifelong infections caused by these human herpesviruses,except for varicella-zoster virus(VZV)vaccine.However,the strict host tropism of herpes-viruses and other limitations mean that no single animal model can fully replicate all key features of human herpesvirus-associated diseases.This makes it challeng-ing to evaluate vaccines and antivirals against human herpesvirus comprehensively.Herein,we summarize the current animal models used to study the human herpesvi-ruses includingα-herpesviruses(herpes simplex virus type 1(HSV-1),HSV-2,VZV),β-herpesviruses(human cytomegalovirus(HCMV),γ-herpesviruses(Epstein-Barr virus(EBV))and Kaposi's sarcoma herpesvirus(KSHV)).By providing concise information and detailed analysis of the potential,limitations and applications of various models,such as non-human primates,mice,rabbits,guinea pigs,and tree shrews,this sum-mary aims to help researchers efficiently select the most appropriate animal model,offering practical guidance for studying human herpesvirus.
