In this study,thyme essential oil(TEO)nanoemulsion(tPTNs)was constructed with transglutaminase(TGase)-modified potato protein,and its antibacterial activity and mechanism of action were evaluated and explored.Results ...In this study,thyme essential oil(TEO)nanoemulsion(tPTNs)was constructed with transglutaminase(TGase)-modified potato protein,and its antibacterial activity and mechanism of action were evaluated and explored.Results indicated that tPTNs exhibited great antibacterial activity against both Staphylococcus aureus and Escherichia coli,with minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of 2.5 and 5.0 mg/mL,respectively.Also,the antibacterial effects of tPTNs were concentration-dependent.We observed a significant decrease in the absolute value of the zeta potential,and significant increases in particle size,cell membrane hydrophobicity,conductivity,the release of metal ions,and the leakage of nucleic acid as the concentration of tPTNs increased from 0 mg/mL to MBC.Furthermore,sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE)demonstrated that protein synthesis was inhibited or even disrupted.Analysis by liquid chromatography-mass spectrometry(LC-MS)indicated that treatment with tPTNs caused significant changes in bacterial metabolites,1117 and 692 differential metabolites being found for S.aureus and E.coli,respectively.The differential metabolites were involved in nucleotide metabolism,amino acid metabolism,tricarboxylic acid cycle and other metabolic pathways.These findings provide valuable insights for the application of thyme essential oil as an efficient antibacterial agent and for the understanding of its mechanism of action.展开更多
Although legal liability is a key component of local regulations and ensures their effective implementation,existing research has paid insufficient attention to the techniques for setting legal liability clauses in th...Although legal liability is a key component of local regulations and ensures their effective implementation,existing research has paid insufficient attention to the techniques for setting legal liability clauses in the local legislation,due to a lack of systematic analysis.To address this gap,this paper employs the interview method to empirically examine the formation process,as well as the techniques for establishing legal liability in local legislation,with the aim of revealing the primary legislative techniques for formulating legal liability clauses in local legislation and their application principles.Drawing from these empirical findings and analytical insights,the research demonstrates that the legal liability-setting techniques in local legislation tend to exhibit a weak coherence with regard to logic and a strong policy orientation.These issues potentially create tension with the legal norms’demands for certainty,abstraction,and completeness.Therefore,this paper proposes a three-pronged framework to harmonize competing demands by respecting local particularities and achieving context-sensitive regulatory balance.This framework advocates that local legislative practice should(a)prioritize practical rationality and rule effectiveness over logical completeness,acknowledging inherent conflicts with traditional legislative models;(b)adopt a typological legislative method to balance normative principles with governance efficiency by transcending logic-experience binaries;and(c)establish a dynamic updating mechanism with regular evaluation to maintain adaptability to socio-economic evolution.展开更多
针对现有知识推理补全方法在可解释性、计算效率和不可见实体预测三者间无法兼顾的问题,提出了基于中心路径结构的PGOCZ(Path Graph Of Central Zone)。PGOCZ利用实体局部结构上路径间的关联性和无关性,构建相互关联的路径结构作为可解...针对现有知识推理补全方法在可解释性、计算效率和不可见实体预测三者间无法兼顾的问题,提出了基于中心路径结构的PGOCZ(Path Graph Of Central Zone)。PGOCZ利用实体局部结构上路径间的关联性和无关性,构建相互关联的路径结构作为可解释性依据;通过关注实体局部路径间的关联与差异,避免重复子路径,提高计算效率;利用局部路径标识相对位置与语义信息,预测不可见实体。PGOCZ在归纳推理任务FB15k-237数据集和转导推理任务NELL-995数据集上表现最佳,且在多数据集上均能以不到30%的数据量达到稳定,有效兼顾了可解释性、计算效率和图谱外实体预测,验证了模型的有效性。展开更多
Based on the WoS and CNKI databases,this paper provides statistical analysis of Chinese and international polar research from 2013-2018 in terms of the number of publications,citations,institutions,disciplinary distri...Based on the WoS and CNKI databases,this paper provides statistical analysis of Chinese and international polar research from 2013-2018 in terms of the number of publications,citations,institutions,disciplinary distribution,collaborations,source journals,and highly cited papers by bibliometric methods.Combining the differences of the traditional disciplinary advantages and natural geographical locations of China and the West,the current research hotspots in the polar field are summarized.(1)"Polar ice cap instability and sea level change","sensitivity and vulnerability of polar ecosystems","Arctic sea-ice-air interactions and climate effects"are the focus of research in the world.(2)Political environment research such as"National Strategy and Cooperation under the'Silk Road on Ice'Initiative"has become a unique polar strategic topic in China.At the same time,insights and suggestions are put forward from three aspects,such as increasing policy support,building cooperation system and optimizing strategic layout.展开更多
文摘In this study,thyme essential oil(TEO)nanoemulsion(tPTNs)was constructed with transglutaminase(TGase)-modified potato protein,and its antibacterial activity and mechanism of action were evaluated and explored.Results indicated that tPTNs exhibited great antibacterial activity against both Staphylococcus aureus and Escherichia coli,with minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of 2.5 and 5.0 mg/mL,respectively.Also,the antibacterial effects of tPTNs were concentration-dependent.We observed a significant decrease in the absolute value of the zeta potential,and significant increases in particle size,cell membrane hydrophobicity,conductivity,the release of metal ions,and the leakage of nucleic acid as the concentration of tPTNs increased from 0 mg/mL to MBC.Furthermore,sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE)demonstrated that protein synthesis was inhibited or even disrupted.Analysis by liquid chromatography-mass spectrometry(LC-MS)indicated that treatment with tPTNs caused significant changes in bacterial metabolites,1117 and 692 differential metabolites being found for S.aureus and E.coli,respectively.The differential metabolites were involved in nucleotide metabolism,amino acid metabolism,tricarboxylic acid cycle and other metabolic pathways.These findings provide valuable insights for the application of thyme essential oil as an efficient antibacterial agent and for the understanding of its mechanism of action.
文摘Although legal liability is a key component of local regulations and ensures their effective implementation,existing research has paid insufficient attention to the techniques for setting legal liability clauses in the local legislation,due to a lack of systematic analysis.To address this gap,this paper employs the interview method to empirically examine the formation process,as well as the techniques for establishing legal liability in local legislation,with the aim of revealing the primary legislative techniques for formulating legal liability clauses in local legislation and their application principles.Drawing from these empirical findings and analytical insights,the research demonstrates that the legal liability-setting techniques in local legislation tend to exhibit a weak coherence with regard to logic and a strong policy orientation.These issues potentially create tension with the legal norms’demands for certainty,abstraction,and completeness.Therefore,this paper proposes a three-pronged framework to harmonize competing demands by respecting local particularities and achieving context-sensitive regulatory balance.This framework advocates that local legislative practice should(a)prioritize practical rationality and rule effectiveness over logical completeness,acknowledging inherent conflicts with traditional legislative models;(b)adopt a typological legislative method to balance normative principles with governance efficiency by transcending logic-experience binaries;and(c)establish a dynamic updating mechanism with regular evaluation to maintain adaptability to socio-economic evolution.
文摘针对现有知识推理补全方法在可解释性、计算效率和不可见实体预测三者间无法兼顾的问题,提出了基于中心路径结构的PGOCZ(Path Graph Of Central Zone)。PGOCZ利用实体局部结构上路径间的关联性和无关性,构建相互关联的路径结构作为可解释性依据;通过关注实体局部路径间的关联与差异,避免重复子路径,提高计算效率;利用局部路径标识相对位置与语义信息,预测不可见实体。PGOCZ在归纳推理任务FB15k-237数据集和转导推理任务NELL-995数据集上表现最佳,且在多数据集上均能以不到30%的数据量达到稳定,有效兼顾了可解释性、计算效率和图谱外实体预测,验证了模型的有效性。
文摘Based on the WoS and CNKI databases,this paper provides statistical analysis of Chinese and international polar research from 2013-2018 in terms of the number of publications,citations,institutions,disciplinary distribution,collaborations,source journals,and highly cited papers by bibliometric methods.Combining the differences of the traditional disciplinary advantages and natural geographical locations of China and the West,the current research hotspots in the polar field are summarized.(1)"Polar ice cap instability and sea level change","sensitivity and vulnerability of polar ecosystems","Arctic sea-ice-air interactions and climate effects"are the focus of research in the world.(2)Political environment research such as"National Strategy and Cooperation under the'Silk Road on Ice'Initiative"has become a unique polar strategic topic in China.At the same time,insights and suggestions are put forward from three aspects,such as increasing policy support,building cooperation system and optimizing strategic layout.
