Previous research has demonstrated the feasibility of repairing nerve defects through acellular allogeneic nerve grafting with bone marrow mesenchymal stem cells.However,adult tissue–derived mesenchymal stem cells en...Previous research has demonstrated the feasibility of repairing nerve defects through acellular allogeneic nerve grafting with bone marrow mesenchymal stem cells.However,adult tissue–derived mesenchymal stem cells encounter various obstacles,including limited tissue sources,invasive acquisition methods,cellular heterogeneity,purification challenges,cellular senescence,and diminished pluripotency and proliferation over successive passages.In this study,we used induced pluripotent stem cell-derived mesenchymal stem cells,known for their self-renewal capacity,multilineage differentiation potential,and immunomodulatory characteristics.We used induced pluripotent stem cell-derived mesenchymal stem cells in conjunction with acellular nerve allografts to address a 10 mm-long defect in a rat model of sciatic nerve injury.Our findings reveal that induced pluripotent stem cell-derived mesenchymal stem cells exhibit survival for up to 17 days in a rat model of peripheral nerve injury with acellular nerve allograft transplantation.Furthermore,the combination of acellular nerve allograft and induced pluripotent stem cell-derived mesenchymal stem cells significantly accelerates the regeneration of injured axons and improves behavioral function recovery in rats.Additionally,our in vivo and in vitro experiments indicate that induced pluripotent stem cell-derived mesenchymal stem cells play a pivotal role in promoting neovascularization.Collectively,our results suggest the potential of acellular nerve allografts with induced pluripotent stem cell-derived mesenchymal stem cells to augment nerve regeneration in rats,offering promising therapeutic strategies for clinical translation.展开更多
为实现工业固废在道路与铁道路基工程中的高值化利用,助力双碳目标在交通运输工程领域中的达成,采用钢渣、矿渣与2种脱硫产物(DP-1、DP-2)等工业固废制备再生胶凝固化剂。基于响应面法考察不同工业固废的交互作用并得到其最优组配。采...为实现工业固废在道路与铁道路基工程中的高值化利用,助力双碳目标在交通运输工程领域中的达成,采用钢渣、矿渣与2种脱硫产物(DP-1、DP-2)等工业固废制备再生胶凝固化剂。基于响应面法考察不同工业固废的交互作用并得到其最优组配。采用最优组配下的固化剂对粉土进行改良固化,并表征本研究固化粉土微观结构。与水泥固化粉土作对比,探究了固化剂掺量、养护龄期对固化粉土力学性能和耐久性的影响规律,并分析2种固化粉土的矿物成分,探究再生胶凝固化剂的粉土固化机理。研究结果表明:7 d无侧限抗压强度(p_(usc))响应面模型经残差正态概率分布检验,模型可靠度较高。钢渣与矿渣、钢渣与DP-2交互作用对7 d p_(usc)值的影响极其显著,再生胶凝固化剂最优配比(%)为钢渣∶矿渣∶DP-1∶DP-2=52.1∶19.9∶19.5∶8.5。研究可知,固化粉土具有良好的力学性能和耐久性,在养护后期(28 d)与水泥固化粉土p_(usc)值、加州承载比值、水稳性能系数值、冻融循环系数值分别相差1.9%、0.5%、1.0%、2.3%。再生胶凝固化剂组分在碱性环境中发挥协同作用,水化产物交错生长填充了粉土颗粒之间的孔隙,从而提高了固化粉土的力学性能。同掺量的再生胶凝固化剂碳排放仅为水泥的1.66%,推荐再生胶凝固化剂路床固化工程最优掺量为6%~8%,该技术的推广应用具有重要环保和社会意义。展开更多
基金supported by the National Natural Science Foundation of China,No.32171356(to YW)Self-Support Research Projects of Shihezi University,No.ZZZC2021105(to WJ)+1 种基金Capital Medical University Natural Science Cultivation Fund,No.PYZ23044(to FQM)Beijing Municipal Natural Science Foundation,No.7244410(to JHD)。
文摘Previous research has demonstrated the feasibility of repairing nerve defects through acellular allogeneic nerve grafting with bone marrow mesenchymal stem cells.However,adult tissue–derived mesenchymal stem cells encounter various obstacles,including limited tissue sources,invasive acquisition methods,cellular heterogeneity,purification challenges,cellular senescence,and diminished pluripotency and proliferation over successive passages.In this study,we used induced pluripotent stem cell-derived mesenchymal stem cells,known for their self-renewal capacity,multilineage differentiation potential,and immunomodulatory characteristics.We used induced pluripotent stem cell-derived mesenchymal stem cells in conjunction with acellular nerve allografts to address a 10 mm-long defect in a rat model of sciatic nerve injury.Our findings reveal that induced pluripotent stem cell-derived mesenchymal stem cells exhibit survival for up to 17 days in a rat model of peripheral nerve injury with acellular nerve allograft transplantation.Furthermore,the combination of acellular nerve allograft and induced pluripotent stem cell-derived mesenchymal stem cells significantly accelerates the regeneration of injured axons and improves behavioral function recovery in rats.Additionally,our in vivo and in vitro experiments indicate that induced pluripotent stem cell-derived mesenchymal stem cells play a pivotal role in promoting neovascularization.Collectively,our results suggest the potential of acellular nerve allografts with induced pluripotent stem cell-derived mesenchymal stem cells to augment nerve regeneration in rats,offering promising therapeutic strategies for clinical translation.
文摘为实现工业固废在道路与铁道路基工程中的高值化利用,助力双碳目标在交通运输工程领域中的达成,采用钢渣、矿渣与2种脱硫产物(DP-1、DP-2)等工业固废制备再生胶凝固化剂。基于响应面法考察不同工业固废的交互作用并得到其最优组配。采用最优组配下的固化剂对粉土进行改良固化,并表征本研究固化粉土微观结构。与水泥固化粉土作对比,探究了固化剂掺量、养护龄期对固化粉土力学性能和耐久性的影响规律,并分析2种固化粉土的矿物成分,探究再生胶凝固化剂的粉土固化机理。研究结果表明:7 d无侧限抗压强度(p_(usc))响应面模型经残差正态概率分布检验,模型可靠度较高。钢渣与矿渣、钢渣与DP-2交互作用对7 d p_(usc)值的影响极其显著,再生胶凝固化剂最优配比(%)为钢渣∶矿渣∶DP-1∶DP-2=52.1∶19.9∶19.5∶8.5。研究可知,固化粉土具有良好的力学性能和耐久性,在养护后期(28 d)与水泥固化粉土p_(usc)值、加州承载比值、水稳性能系数值、冻融循环系数值分别相差1.9%、0.5%、1.0%、2.3%。再生胶凝固化剂组分在碱性环境中发挥协同作用,水化产物交错生长填充了粉土颗粒之间的孔隙,从而提高了固化粉土的力学性能。同掺量的再生胶凝固化剂碳排放仅为水泥的1.66%,推荐再生胶凝固化剂路床固化工程最优掺量为6%~8%,该技术的推广应用具有重要环保和社会意义。
