摘要
采用继代培养三个月后的桑子叶悬浮细胞为材料,进行了原生质体的分离和培养。桑悬浮细胞在纤维素酶、果胶酶、半纤维素酶的混合溶液中,酶解获得产量高、活力强的原生质体。原生质体在K8p(附加6—BA、NAA、2,4—D、LH)液体培养基中,再生细胞经多次分裂,得到肉眼可见的小愈伤组织。再通过增殖继代培养,获得浅黄色、具有明显颗粒结构的愈伤组织,转至各种激素含量的MSB固体培养基中,尚未获得绿苗分化。
This paper reported the callus formation from the protoplasts isolated from the suspension cells, which have been subcultured for 3 months, of mulberry cotyledons. Numerous active protoplasts were isolated in an enzyme solution consisting of cel-lulase.pectinase and hemicellulase. The isolated protoplasts cultured in K8p.liquid medium containing 6-BA ,NAA, 2,4-D and LH started to divide and formed small calli perceived by naked eyes. These small calli grew rapidly in proliferation medium and became yellowish and nodular. After they were transfered into MSB medium with different hormone composition,no bud was induced yet.
出处
《蚕业科学》
CAS
CSCD
1993年第3期135-138,共4页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金资助项目
