摘要
对稀有植物裸果木进行组织培养研究,在MS培养基上裸果木的下胚轴脱分化形成愈伤组织,并进一步分化形成再生植株。激素种类及其浓度是器官脱分化与植株再生的决定因素。诱导下胚轴形成愈伤组织的最适培养基为MS+1mg/L6-BA+0.5mg/LNAA;芽分化诱导的最适培养基为MS+1mg/L6-BA;生根的最适培养基为不含任何激素的1/2MS培养基。
Tissue culture about rare plant Gymnocarpos przewalskii has been carried out.The calli from hypocotyl of Gymnocarpos przewalskii could be induced and plantlet were regenerated on MS medium.Hormone kinds and their concentration in MS medium was the decisive factor in determination organ dedifferentiation and redifferentiation.The optimum medium of callus inducing from hypocotyl was MS+2 mg/L 6-BA+0.5 mg/L NAA;The optimum medium of inducing bud was MS+6-1 mg/L BA+0.05 mg/L NAA;The optium medium of rooting was hormone-free 1/2 MS medium.
出处
《西北植物学报》
CAS
CSCD
2004年第7期1319-1321,共3页
Acta Botanica Boreali-Occidentalia Sinica
关键词
裸果木
稀有植物
组织培养
植株再生
Gymnocarpos przewalskii Maxim.
rare plant
tissue culture
plantlet regeneration
