摘要
利用PCR技术对烟粉虱与温室白粉虱内共生菌进行分子比较研究 ,筛选出C、D、G和H等 4对引物 ,分别能够扩增出烟粉虱内共生菌的groEL基因、初生内共生菌与次生内共生菌的1 6SrDNA基因 ,与GenBank中相应序列的同源性分别为 99%、99 5 8%、98%和 98% ,而不能从温室白粉虱内共生菌中扩增出该类基因 。
PCR technique was used to distinguish Bemisia tabaci from Trialeurodes vaporariorum. The results showed that C,D,G and H special primers were acquired,C and D for groEL,G and H for 16Sr DNA of primary endosymbiont and secondary endosymbiont from Bemisia tabaci were amplified respectively,and similarity between the sequences from the four primers and the sequences from GenBank is 99%,99^58%,98% and 98%.But the four primers were not suitable for amplifying from Trialeurodes vaporariorum ,indicating that the endosymbionts were different between Bemisia tabaci and Trialeurodes vaporariorum.
出处
《核农学报》
CAS
CSCD
北大核心
2004年第3期237-240,共4页
Journal of Nuclear Agricultural Sciences
基金
国家重点基础研究发展规划项目 ( 2 0 0 2CB1114 0 1)
农业部农作物病虫草害生物防治资源研究与利用重点开放实验室开放基金 (LOBCR 2 0 0 3 0 1)
关键词
烟粉虱
温室
白粉虱
共生菌
分子比较
Bemisia tabaci
Trialeurodes vaporariorum
endosymbionts
molecular classification
