摘要
柑橘黄龙病(Huanglongbing,HLB)是经柑橘木虱Diaphorina citri传播的最主要柑橘病害之一,危害严重时能对柑橘产业造成毁灭性的破坏。为了鉴定福建和海南2个地理种群柑橘木虱的内共生菌群落组成,本研究对16SrRNA部分保守序列进行PCR扩增,并利用特异性引物对不同内共生菌进行了感染率检测;另外,还通过人工接虫的方法,探索柑橘木虱成虫在带黄龙病菌蕉柑Citrus reticulata cv.Tankan上的获菌能力,以及带菌柑橘木虱成虫对黄岩蜜橘C.reticulata cv.Subcompress的传菌能力。研究发现,这2个地理种群的柑橘木虱含有相同的内共生菌组成,包括α-Proteobacteria,Wolbachia spp.,γ-Proteobacteria,mycetocyte symbionts,β-Proteobacteria,Oxalobacter和β-Proteobacteria,Herbaspirillum,而且这2个地理种群柑橘木虱的4种内共生菌的携带率均在95%以上。柑橘木虱成虫在带菌蕉柑上饲菌28d后,带菌率可达到82%,而带菌柑橘木虱成虫在黄岩蜜橘上传菌75d后,可导致橘树整体带菌。本研究为柑橘木虱的进一步研究和防虫治病途径提供了一些理论依据。
Huanglongbing （HLB） is one of the most severe citrus diseases, which is usually transmitted by the Asian citrus psyllid （Diaphorina citri）, and causes great economic loss. To understand the endosymbiotic microbiota of the citrus psyllid from Hainan and Fujian provinces in China, the partial conserved region of 16S rRNA was amplified by PCR, and subsequently the infection rate of each endosymbiont from two geographical populations of the citrus psyllid was detected by PCR using specific primer pairs in this stduy. Moreover, we detected the acquisition efficiency of the pathogen by adult citrus psyllid reared on Citrus reticulata cv. Tankan infected with Candidatus Liberibacter asiaticus （CLas）, and the transmission efficiency to Citrus reticulata cv. Subcompress via CLas-positive psyllids. The results demonstrated that the two geographical populations of the citrus psyllid had the same composition of endosymbiotic microbiota, including α-Proteobacteria, Wolbachia spp., γ-Proteobacteria, mycetocyte symbionts, β-Proteobacteria, Oxalobacter, and β-Proteobacteria, HerbaspiriUum, and the infection rate of each endosymbiont from the two geographical populations was more than 95%. After the adult citrus psyllids were reared on CLas-infected C. reticulata cv. Tankan for 28 d, the acquisition efficiency was as high as 82%. The whole tree of C. reticulata cv. Subcompress was infected after rearing the CLas-positive psyllids on it for 75 d. The results would provide some theoretical basis for the study of citrus psyllid and the effective control of HLB.
出处
《昆虫学报》
CAS
CSCD
北大核心
2012年第10期1149-1153,共5页
Acta Entomologica Sinica
基金
农业公益性项目"柑橘黄龙病和溃疡病综合防控技术研究与示范"(201003067)
关键词
柑橘
柑橘黄龙病
柑橘木虱
内共生菌
传菌
Citrus
Huanglongbing （HLB）
Diaphorina cirri
endosymbiont
pathogen transmission
