摘要
以抗蚜高粱———河农16为试材,采用单因素筛选的方法,摸索SSR反应体系中各个影响因素的浓度和用量,然后采用完全随机试验,进行优化组合,最终获得适宜高粱抗蚜虫基因定位的SSR体系。其中TaqDNA聚合酶0 3μL(5u μL),10×PCRBuffer(Mg2+)2 0μL,引物1 5μL(10μmol L),模板DNA60ng。利用该体系进行扩增,所得谱带清晰、稳定、非特异性带少。
A high resistant material to greenbug in sorghum—Henong 16 was used in this expirement. Single factor selection was made to investigate better concentration in SSR technique system. Then, random thoroughly experiment was made to seek the best combination among factors. At last, the best SSR technique system was established. Taq DNA polymerase 0.3 μL(5 u/μL ),10×PCR Buffer(Mg^(2+)) 2.0 μL,primer 1.5 μL (10 μmol/L),template DNA 60 ng. Using this system, amplification bands were clear, standard and non-especial bands were shorter.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2004年第3期21-24,共4页
Journal of Hebei Agricultural University
基金
国家自然科学基金资助项目(30170498)
关键词
高粱
抗蚜
SSR
体系优化
sorghum
resistant of greenbug
SSR
system establishing
