摘要
目的在大鼠局灶性脑挫裂伤模型上,研究脑挫裂伤组织中凝血酶对周围脑水肿的影响。方法选择健康成年SD大鼠60只,随机分为四组对照组(TBI组),凝血酶(T)组,全血(WB)组和生理盐水(S)组。各组又按其存活时间分为注入后1,3,5d三个亚组,分别于注入不同物后24,72,120h处死,取挫裂伤区脑组织测其含水量及钠含量。结果脑外伤后第1天,T组与WB组脑含水量和脑组织钠含量较其他两组明显增高;脑外伤后第3,5天,WB组脑含水量和脑组织钠含量较其他三组明显增高。T组在伤后第1天的脑含水量和脑组织钠含量最高,在第3,5天逐渐下降;WB组在伤后第3天的脑含水量和脑组织钠含量最高。结论脑挫裂伤伤灶中的凝血酶可加重伤灶周围的脑水肿,第1天为水肿的最高峰,第3天仍可见水肿,第5天水肿明显消退。脑挫裂伤伤灶中的全血和凝血酶在注入后的第1天均可导致脑水肿,且相比较无差异,提示全血在注入后的第1天所致的脑水肿可能与血肿中的凝血酶有关。
Objective:To study the changes of water and sodium contents of brain tissue at different time,and to detect the effects of thrombin on brain edema using traumatic brain injury animal model.Meth-ods:Sixty healthy adult rats were subjected to controlled cortical impact and randomly divided into four groups(15in each group):the control group,and the other three groups were injected with50μl saline,10U thrombin and50μl whole autologous,blood respectively.All rats were sacrificed at different time and brain water and sodium contents were measured.Results:The water and sodium contents of brain in group T and WB were significantly higher than those in group TBI and S at the first day after TBI.The peak value of water and sodium contents of the brain in group WB was observed at the3rd after injury.Conclusions:The thrombin in the injured tissue of cerebral contusion results in a more serious brain edema,which reachs the peak at the1st day and the edema sustained to the3rd day.The results suggest that the thrombin may be the key factor inducing brain edema at the1st day after TBI.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第11期88-91,共4页
China Journal of Modern Medicine
关键词
创伤性脑损伤
脑内血肿
脑水肿
凝血酶
鼠
thrombin
brain edema
intracerebral hematoma
traumatic brain injury
rat
