摘要
目的探讨脑出血后血肿周围组织神经肽Y含量的变化及尼膜同对其表达的影响。方法采用未抗凝新鲜自体股动脉血注入大鼠尾状核建立脑出血动物模型。将75只大鼠随机分成对照组、脑出血组、尼膜同组,各实验组分6h、1d、3d、5d和7d5个时间点。分别采用干重湿重法、放射免疫法测定不同时间点的脑组织水含量和神经肽Y表达的变化。结果脑组织水含量和神经肽Y表达均在ICH后6h开始升高,3d达到高峰,5～7d开始下降,两者之间存在明显的相关性(r=0.78,P<0.01)。尼膜同能够降低各个时间点的脑组织水含量和神经肽Y的表达。结论尼膜同可以降低脑出后血肿周围脑组织神经肽Y的活性,减轻ICH后的脑水肿形成。神经肽Y与脑水肿的形成密切相关,阻断神经肽Y活性可能是ICH后脑水肿治疗的一种可行方法。
[Objective] To investigate dynamic changes of water content and neuropetie Y （NPY） in Intracerebral hemorrhagic （ICH） rats and the role that neuropetie Y plays in cerebral edema after hemorrage, and to evaluate the effect of Nimotop in reducing the content of cerebral edema and neuropetie Y. [Methods] Animal model was made by injecting self arterial blood into the caudate nucleus of rats. 75 rats were randomly assigned to control group, cerebral hemorrhage group and nimodipine treated group. After the injection, the rats were sacrificed at 6 h, 1 d, 3 d, 5 d, 7 d respectively. Brain water content was determined by wet-dry weight, as the expression of neuropetie Y was detected by radioimmunoassay method over a time course ranging from 6 h to 7 d. [Results] Brain water content and neuropetie Y increased after 6 h of cerebral hemorrhage and reached its climax at the 3rd day, then decreased at the 5th to the 7th day. and there is an obvious correlation between them （r =0.815, P 〈0.01）. Administration of Nimotop could reduce the content of brain edema and neuropetie Y. [ Conclusion ] Nimotop can reduce neuropetie Y expression, thus can reduce cerebral edema after ICH. These suggest a close correlation between neuropetie Y and cerebral edema, Nimotop antagonist might be available for the therapy of cerebral edema after ICH.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第6期662-664,共3页
China Journal of Modern Medicine
基金
2005年博士科研启动
自然科学基金项目(20052095)
关键词
脑出血
神经肽Y
尼膜同
脑水肿
Intracerebral hemorrhage
europeptid Y
cerebral edema
Nimotop
