摘要
香木瓜环斑病毒(PRV)外壳蛋白(CP)基因cDNA克隆到pUC18上构建成大肠杆菌(Escherickia coli)表达载体。Westrn blot检测结果表明该表达载体在B.coli DH5 a中有两种特异蛋白表达,分于量为34 kd的蛋白与推测的表达产物大小相符,且与PRVCP的一个“组分”大小相似;面分子量为16 kd的蛋白可能是34 kd蛋白的降解物或者是PRV CP基因不完全表达产物。
E. coli DH5α was transformed by pUC 18 recombined with PRV CP gene cDNA. Western blot showed that two novel proteins were expressed from the recombinant, one with MW34 kd conformed in size to the predicted expression product, and approximated that of a PRV CP 'fraction', the other protein was probably a cleavage product of the 34kd protein or one resulting from partial expression of the PRV CP gene.
出处
《华南农业大学学报》
CAS
CSCD
1992年第1期6-9,共4页
Journal of South China Agricultural University
基金
国家自然科学基金
