摘要
对影响微甘菊愈伤组织诱导的因素进行了系统研究.结果表明:芽尖和嫩茎作为外植体时诱导率较高,最适诱导培养基是MS培养基附加2 0mg/L2,4-D+1 0mg/LKT,pH值5 7,(25±1)℃条件下黑暗诱导.
Factors affecting induction of callus in Mikania micrantha Kunth were studied in the paper. The results showed that auxiliary shoot and bud tip had a higher level of callus induction ratio; the best callus induction medium was MS with the addition of 2,4-D2.0mg/L+KT1.0mg/L, pH 5.7;with the culture being kept dark under(25±1)℃.
