摘要
目的 :探讨姜黄素 (curcumin)对人宫颈癌细胞株Hela细胞体外增殖及凋亡的调控作用。方法 :选用人宫颈癌Hela细胞进行体外培养 ,以 5～ 5 0 μmol/L的姜黄素分别处理Hela细胞 2 4～ 72h后 ,MTT法检测细胞增殖 ,流式细胞仪 (FCM )检测凋亡 ,透射电镜进行形态学观察。SABC免疫组化法检测细胞内Bcl- 2、BcI -XL、Caspase - 3蛋白表达水平。结果 :curcumin对Hela细胞增殖有抑制作用。并呈剂量依赖性。DNA直方图上可见亚“G1”峰 ;透射电镜观察见部分细胞发生凋亡形态学改变。Bcl- 2、Bcl-XL表达均下调 ,Caspase - 3表达增强。 结论 :姜黄素对人宫颈癌Hela细胞的增殖具有明显抑制作用。上调Cas pase - 3,下调Bcl- 2、Bcl-XL蛋白的表达 ,诱导细胞凋亡可能是其作用机制之一。
Objective:To investigate the regulatory effect of curcumin on proliferation and apoptosis in human cervical carcinoma cell line Hcla in vitro.Methods:Human cervical carcinoma cell line Hela was cultured in vitro.Hela cells were treated with 5-50 μmol/L curcumin for 24-72h and the growth inhibition rates of Hela cells were measured with MTT method.Cell apoptosis was inspected by electron microscopy.In addition,the expression of bcl-2,bcl-xl and caspase-3 protein in Hela cell were observed by SABC immunohistochemistry.Results:Curcumin inhibited the proliferation of Hela cells on a dose-depending manner.With the peak of sub G 1 appeared on DNA histogram in FCM.Partial cells presented the characteristic morphological changes of apoptosis under the electron microscope.The Bcl-2, Bcl-xl expression were decreased while Caspase-3 expression was increased.Conclusion:Curcumin could significantly inhibit the growth of Hela cells;inducing apoptosis through up-regulating Caspase-3 and down-regulating expression of Bcl-2,Bcl-xl is probably one of its molecular mechanisms.
出处
《重庆医科大学学报》
CAS
CSCD
2004年第3期296-298,314,共4页
Journal of Chongqing Medical University
关键词
姜黄素
子宫颈肿瘤
凋亡
Curcumin
Cervix neoplasm
Apoptosis
