摘要
目的:探讨孕激素(P)对人宫颈癌细胞株Hela细胞体外增殖及凋亡的影响。方法:体外培养人宫颈癌Hela细胞,实验组加入不同浓度孕酮,对照组加入不含孕酮的培养液,应用四甲基偶氮唑蓝(MTT)比色法观察对细胞增殖的影响,光镜下观察其形态学变化。流式细胞仪(FCM)检测细胞周期变化及细胞凋亡比。结果:当孕酮浓度为1-10μmol/L时,作用48、72小时,对细胞生长有明显抑制作用(P<0.01),并具有剂量依赖性。流式细胞仪检测显示孕酮组G0/G1期细胞比例上升,S期细胞比例下降,凋亡比上升。光学显微镜下可观察到细胞凋亡的形态学改变。结论:孕酮抑制宫颈癌Hela细胞增殖,并诱导凋亡。
Objective： To investigate the effects of progesterone （P） on the proliferation and apoptosis of Hela cer- vical cancer cell lines in vitro. Methods： Hela cells were cultured in vitro. Two groups were set up ： study group （progesterone in different concentrations） and control group without progesterone. Hela cell proliferation was meas- ured by the methyl thiazolyl tetrazolium （MTY） method. Cell cycle and apoptosis percentage were detected by flow cytometry. Morphological changes of apoptotic ceils were observed by light microscopy. Results： Progesterone of 1 - 10μmol/L inhibited Hela cells growth in a dose - dependent manner（ P 〈 0.01 ）, cells were incubated with progester- one for 24 or 48 hours. FCM showed that progesterone increased the proportion of G0/G1 phase, reduced the proportion of S phase, and the apoptotic rate increased significantly in progesterone group. By hght microscopy, many morphlolgi- cal characteristics of apoptosis were observed in progesterone group. Conclusion： Progesterone can inhibit the prolif- eration of Hela cells and induce apoptosis.
出处
《现代肿瘤医学》
CAS
2012年第7期1334-1336,共3页
Journal of Modern Oncology
基金
北京市海淀区科技基金项目(基金编号:K2008058)
关键词
宫颈肿瘤
孕酮
细胞增殖
细胞凋亡
I cervix neoplasms
progesterone
cell prolifercation
apoptosis
