摘要
探讨了体外连续培养的人胚肺二倍体细胞在低剂量苯并芘长时期作用下超微结构的早期癌特征。结果发现:低剂量(10^(-7)及10^(-8)mol)苯并芘(Bap)长时期(23～38代)作用下,光镜检查未见细胞有明显癌变征,而电镜下发现细胞核外形不规则,核膜深陷,有者呈复杂折叠及套叠,出现细桥伴分叶核形成,以及核内胞浆包涵体(或称核内假包涵体),核内的常染色质一般丰富;核仁巨大或多核仁等癌细胞特征。说明用电镜可观察到在致癌物作用下细胞早期癌变的征象。实验结果对进一步研究致癌物在低剂量、长时期作用下的致癌机理和预防措施将有重要意义。
This paper approachs the early cancer characteristic of ultrastructure features of human embryolung under Benzo(a)pyrene(Bap) with low dose and long term. No obvious indication of carcinomacan be found by light microscope. However, by means of electron microscope, some early cancer char-icteristics can be observed as follows: irregular appearance of nucleus, complex folds and invagenationsof nuclear envelope, abundant euchromatin, giant nucleoli and nucleolus, the formation of tiny bridgesin company with nuclear lobulus and intranuclear pseudoinclusions as well. This experimental rcsultplays an important role in the further research of carcinogenic mechanism and prevention measurmensunder the action of carcinogen with low dose and long term
出处
《北京医科大学学报》
CSCD
1993年第5期329-331,395,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
肺
二倍体细胞
培养
超微结构
Dipoid cell
Human emoryo lung cell
Benzo(a) pyrene
Low dose
Ultrastructure
