摘要
In this report, a universal protocol for extract-ing genomic DNA from whole blood, saliva, and bacterial culture by using magnetic nanobeads as solid-phase absor-bents was presented. The enrichment of target cells and ad-sorption of DNA have been functionally integrated onto the surfaces of the carboxyl-modified magnetic nano-beads, and the DNA segments bound on the surface of the beads can be directly used as PCR templates to amplify a target gene. The PCR products were applied to an oligonucleotide array to perform gene typing. The protocol proves to be simple, rapid, biologically and chemically nonhazardous, and promising for the microfabrication of DNA preparation chip.
In this report, a universal protocol for extract-ing genomic DNA from whole blood, saliva, and bacterial culture by using magnetic nanobeads as solid-phase absor-bents was presented. The enrichment of target cells and ad-sorption of DNA have been functionally integrated onto the surfaces of the carboxyl-modified magnetic nano-beads, and the DNA segments bound on the surface of the beads can be directly used as PCR templates to amplify a target gene. The PCR products were applied to an oligonucleotide array to perform gene typing. The protocol proves to be simple, rapid, biologically and chemically nonhazardous, and promising for the microfabrication of DNA preparation chip.
