摘要
以HBV DNA的克隆菌E.coli.JM83为出发菌株,对比研究了三种纯化细菌质粒DNA的方法。实验结果表明,CsCl密度梯度超速离心法的得率和产品纯度均佳,但操作繁琐,花费昂贵;Sepharose 4B柱层析纯化法简便快速又勿需专门仪器,缺点是产品纯度不够高;电泳洗脱法的得率虽不及超速离心法,但产品纯度与之相同,由于操作简便,花费大大降低,因而适合普遍采用。
Three methods of purifying bacterial plasmid DNA from HBV DNA cloned E.
coli JM83 were comparatively studied. The results showed that: 1. the produc-
tion and purity of CsCl density gradient ultracentrifugation were the best, but
the procedures were too complecated and expensive; 2. Sepharose 4 B chro-
matography was convenient and rapid. The method didn't need special instru-
ments, but the disadvantage was that the product was not very pure; 3. the
purity of electroelution was the same as that of CsCl ultracentrifugation, but
the production was not so high. Because of its convenient operation and lower
cost, electroelution was comparatively fit for the wide. use of purifying bac-
terial plasmid DNA.
出处
《河南医科大学学报》
1989年第3期242-245,249,共5页
Journal of Henan Medical University
关键词
细菌质粒
DNA
纯化
质粒
plasmid DNA
CsCl density gradient ultracentrifugation
Sepharose 4 B chromatography
eletroelution
