摘要
利用筛选出的 12个随机引物 ,对来源于不同国家和地区的有代表性的 5 6份萝卜种质资源遗传多样性进行了RAPD分析 ,共扩增出 10 9条带 ,其中多态性带为 72条 ,多态性带百分率为 6 2 .9% ,种质平均期望杂合度为 0 .2 89。系统聚类分析将供试材料分为 2大类、9组 ,主坐标分析将其分为 3大类、7组 ,两种分类方法结果基本一致 ,后者能更为直观和清楚地反映群体之间的关系。
The genetic diversity of radish germplasm resources was analyzed with RAPD. A total of 109 distinct bands were detected with 12 informative RAPD primers among 56 radish accessions from different countries or regions. Of them, 72 bands(62.9%) were polymorphic. The average heterozygosity of RAPD loci was 0.289 among 56 accessions. All accessions were clustered into two groups and nine subgroups based on the RAPD data by the method of clustering analysis, and three groups and seven subgroups by the method of principal coordinates analysis. The results from these two methods were similar, but the result from the principal coordinate analysis was more informative.
出处
《植物遗传资源学报》
CAS
CSCD
2004年第2期156-160,169,共6页
Journal of Plant Genetic Resources
基金
国家基础性工作项目 ( 2 0 0 2JCXGZ 19)
农业部农作物资源保护项目 (NB0 3 6)
关键词
萝卜
种质资源
亲缘关系
RAPD分析
遗传
Raphanus sativus L.
Germplasm resources
RAPD
Clustering analysis
Principal coordinate analysis
