摘要
应用RAPD技术对 8份野生种质和 12份栽培种质进行遗传多样性分析 ,筛选到 18个具有多态性扩增的引物 ,共扩增出 12 8条带。据Nei Li相似系数将 2 0份材料分别聚为野生种质和栽培种质两大类。 5个野生种质聚为野生种质类群 ,12个栽培种质和 3个野生种质聚为栽培种质类群。研究结果表明 ,RAPD技术用于橡胶树种质资源研究 。
The genetic diversity of rubber tree was analyzed by using RAPD. There were 18 primers showing abundant polymorphisms. The 18 primers produced 128 polymorphic bands. The results of cluster analysis based on Nei Li coefficient revealed that these 20 samples were divided into two main groups. Five wild germplasm were clustered into wild germplasm group, and 12 cultivated germplasm and three wild germpladm were clustered into cultivated germplasm group. The results showed that RAPD marker was suitable for study on genetic diversity in rubber tree, and offering the molecular basis, on which the genetic substance of wild rubber tree was transferred to cultivated rubber tree.
出处
《植物遗传资源学报》
CAS
CSCD
2004年第2期128-132,共5页
Journal of Plant Genetic Resources
基金
中国热带农业科学院科技基金项目资助
关键词
橡胶树
种质资源
遗传多样性
分子标记
RAPD
Rubber tree
Germplasm resources
Genetic diversity
Molecular marker
RAPD
