摘要
为探讨牛免疫缺陷病毒(BIV)Tat能否在功能上取代HIVTat,构建用BIVtat取代HIVtat的嵌合人/牛免疫缺陷病毒(pHBIV 2)cDNA,将其转染人源MT4细胞。PCR、RT PCR法检测到嵌合基因组在MT4细胞中可稳定地存在并转录;套式Alu PCR法检测到嵌合基因组可整合到细胞基因组中;RTase活性测定及IFA检测显示,嵌合基因在MT4细胞中得到了翻译。结果表明,HIV的tat基因用BIVtat取代后产生的传染性cDNA克隆,仍能在人源MT4细胞中产生有复制性的重组病毒。
In order to study whether BIV Tat could substitute HIV Tat,the chimeric human/bovine immunodeficiency virus(pHBIV-2)cDNA was constructed by replacing HIV tat with BIV tat, then transfected into human MT_4 cell.The stable existence and transcription of the chimerae in MT_4 cell were demonstrated by PCR and RT-PCR.The integration of chimerae into MT_4 cell was verified by nested Alu-PCR.Reverse transcriptase assay and IFA showed the chimeric genome had been expressed.In conclusion,BIV tat could substitute HIV tat in the chimerae and produce replicative recombinant HBIV-2 virus in MT_4 cell.
出处
《病毒学报》
CAS
CSCD
北大核心
2004年第2期138-142,共5页
Chinese Journal of Virology
基金
国家重点基础研究发展规划项目(G1999054107)
