摘要
以Sambrook的碱裂解法为基础,通过控制菌体培养时间,适当调整提取过程中3种溶液的比例及作用时间,摸索出一种适合于提取苏云金芽孢杆菌质粒DNA的方法.该法与常规碱裂解法相比具有重复性好、质粒DNA质量高等优点.
Based on Sambrook's method, a modified protocal suitable for extracting plasmid from Bacillus thuringiensis was put forward through controlling culture time and regulating the proportion of three main solutions. Compared with routine alkaline lysis, this modified method is more reproducible and reliable.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2004年第2期200-201,共2页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家高技术研究发展计划(2002AA245011)
福建省科技厅(2002N004
2001Z026)资助项目.
