摘要
用离子交换色谱法对枇杷有机酸进行有效分离与测定,EG40淋洗液在线发生器能降低背景电导,获得较好的重现性和平稳的基线.测定结果表明,枇杷果实和叶片中有机酸组分和含量有较大差别.果实中以苹果酸为主,其次为奎尼酸和酒石酸,而柠檬酸、草酸和顺乌头酸等含量较低.叶片中以奎尼酸为主,苹果酸、柠檬酸和草酸含量很低.
Organic acids in loquat samples were adequately determined by ion-exchange chromatography. The performing gradient elution with EG40 eluent generator was used to obtain a flat baseline and better reproducibility. Results showed that the malic acid was the top organic acid in fruit; the seconds were quinic acid and tartartic acid. The others as citric, oxalic, cis-aconitic acid were little in fruit. In leaves, the main organic acid was quinic acid, while citric, malic and oxalic acid were little.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2004年第2期195-199,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省自然科学基金资助项目(B0310008).
关键词
离子交换色谱法
枇杷
果实
叶片
有机酸
柠檬酸
苹果酸
loquat
fruit
organic acid
high-performance ion-exchange chromatography (HPIC)
