摘要
目的:研究神经生长因子(neurongrowthfactor,NGF)对大鼠胚胎脊髓神经细胞的作用。方法:在培养孕18d大鼠胚胎脊髓神经细胞中加入NGF,通过培养细胞并计数存活的脊髓神经细胞集落数,检测超氧化物歧化酶(superoxidedismutase,SOD)的活力、丙二醛的含量,并观察NGF对脊髓神经细胞生长发育的影响。结果:NGF可促进脊髓神经细胞分化、增殖。当NGF为10.0～100.0μg/L时SOD活力显著性高于正常对照组(P<0.01),NGF为1.0～100μg/L时丙二醛含量没有明显变化,能维持丙二醛含量的平衡,当NGF浓度为200μg/L时丙二醛含量明显增高为(1.33±0.05)μmol/g,与对照组(0.98±0.01)μmol/g比较,差异有显著性意义(F=6.89,P<0.05),不能维持丙二醛含量的平衡。培养14d后脊髓神经细胞体较大、突起较长,脊髓神经细胞的数量也明显增高。结论:NGF能促进脊髓神经细胞生长发育,增加脊髓神经细胞的胞体和突起长度,增强脊髓神经细胞内SOD活力,丙二醛含量降低。
AIM:To study the effect of nerve growth factor (NGF) on activities of cultured embryonic spinal cord neurons of rats. METHODS: NGF was added to embryonic spinal cord neurons from rats which was cultivated for 18 days, and the number of survival spinal cord neurons was counted. The activities of superoxide dismutase (SOD) and the contents of malonaldehyde (MDA) was measured and the effect of NGF on the development of cultured embryonic spinal cord neurons in vitro was observed. RESULTS: NGF enhanced the proliferation and differentiation of cultured embryonic spinal. When NGF was 10.0-100.0 μg/L, the SOD activity was significantly higher than that of normal controlled group(P< 0.01); while NGF was 1.0-100 μg/L, MDA content had no obvious changes, and kept its own balance; while NGF concentration was 200 μg/L, MDA content increased significantly(F= 6.89,P< 0.05), and couldn't keep balance. Fourteen days after culture, cell body of spinal cord neuron grew bigger, processes were longer, and the number was increased, too. CONCLUSION:The NGF can promote the growth of spinal cord neurons, enhance the cell body and process length, strengthen the activity of SOD and lower the contents of MDA.
出处
《中国临床康复》
CSCD
2004年第17期3286-3288,i003,共4页
Chinese Journal of Clinical Rehabilitation
基金
江苏省教育厅自然科学基金资助项目(01KJD310006)~~
