摘要
为了探讨CCK卵黄抗体能否被动物消化道所吸收 ,我们应用SDS PAGE电泳、ELISA和Westernblot方法 ,检测灌胃CCK卵黄抗体后SD大鼠十二指肠静脉血液中CCK卵黄抗体的免疫活性及存在形式。实验采用1 4只雄性成年SD大鼠 ,随机分成试验组和对照组 ,分别灌胃 1 5 0mg/mlCCK卵黄抗体粉混悬液和生理盐水 (2ml/只 )。安装亚急性十二指肠静脉插管 ,在灌胃后第 2、 3、 4h采集十二指肠静脉血液 ,分离血浆。SDS PAGE结果显示 ,CCK卵黄抗体分子量为 2 0 0kD。ELISA研究表明 ,试验组第 2、 3h的血浆中 ,均检测到CCK卵黄抗体效价 ,效价达 1∶1 2 8以上。同时 ,Westernblot分析发现 ,试验组第 2、 3h血浆中存在完整分子形式的卵黄抗体。研究结果提示 ,CCK卵黄抗体能被SD大鼠十二指肠段吸收或部分吸收进入血液 。
To clarify whether CCK yolk antibody can be absorbed from duodenum in rats, SDS PAGE electrophoresis, ELISA and Western blot analysis were employed to detect the immunoreactivity and the chemical form of CCK yolk antibody in duodenal venous blood after gastric infusion of CCK yolk antibody. Fourteen adult male SD rats were divided at random into treatment and control groups. Two ml of CCK yolk antibody (150 mg/ml) or physiological solution (0 9% NaCl) was administered intragastrically to rats in treatment and control groups, respectively. Duodenal venous blood was collected from duodenal venous catheters at the 2nd, 3rd and 4th hour after infusion. SDS PAGE electrophoresis showed that the molecular weight of CCK yolk antibody was 200 kD. CCK yolk antibody was detected in the duodenal venous plasma of animals in the treatment group with ELISA at the 2nd and 3rd hour after administration with the titer of 1∶128. Western blot analysis confirmed the finding of ELISA that intact CCK yolk antibody existed in duodenal venous plasma of treatment group. These results suggest that CCK yolk antibody can be absorbed or partly absorbed from the duodenum and play its role in systematic circulation .
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2004年第3期375-379,共5页
ACTA ZOOLOGICA SINICA
基金
香港华扩达生化实业有限公司资助~~
