摘要
选取丹麦成年长白母猪的心脏、肌肉、主动脉、左心室和中国成年二花脸母猪心脏共 5种组织 ,建立相应的cDNA文库 ,在此基础上测定了 35 180条表达序列标签。通过比较分析长白猪 4种组织的基因表达 ,找出了长白猪心脏组织中 3个显著高表达的功能群和 4 7个显著高表达的基因。长白猪心脏组织在分子伴侣活动、马达活动和生理过程中表达的基因较多 ,4 7个显著高表达的基因都是和运动、运动调节、能量及保护有关的基因。两种家猪心脏组织表达差异显著的基因有 74个 ,并且绝大部分是在长白猪心脏中表达更高 ,显示出长白猪心脏组织生理活动的高效与旺盛。
To study the expression profiles in the heart tissues of domestic pigs,five un-normalized cDNA libraries from Danish adult female Landrace heart,biceps femoris,aorta,left ventricle tissues and Chinese adult female Erhualian heart tissue were constructed.Totally 35 180 expressed sequence tags (ESTs) were randomly sequenced.Using functional clustering and statistic methods,three gene clusters and 47 genes highly expressed in Landrace heart tissue were found.These genes are corresponding to the chaperone activity,motor activity and physiology process,implying the functional importance of the protection and movement process in heart.Between the two pig breeds,most of the differentially expressed genes are related with energy metabolism and catalytic activity,of which the majority are highly expressed in Landrace.The results indicate that the physiological activities of Landrace heart are more active and effective than Erhualian heart.
基金
中国-丹麦家猪基因组计划~~
关键词
表达序列标签
表达谱
家猪
心脏
肌肉
expressed sequence tags
expression profile
domestic pig
heart
muscle
