摘要
本文旨在探讨低氧对大鼠内源性硫化氢体系的变化。采用生化反应方法测定血浆中硫化氢的含量和肺组织中硫化氢合酶的活性 ,采用定量竞争性RT PCR的方法检测肺组织中胱硫醚 γ 裂解酶 (CSE)mRNA的含量。结果显示 ,与对照组相比 ,低氧组大鼠的血浆硫化氢含量明显减少 [(1 92 2± 2 2 1 ) μmol Lvs (30 1 6± 32 4 ) μmol L ,P <0 0 1 ) ],肺组织中硫化氢合酶的活性明显下降 [(0 1 2 7± 0 0 2 3)vs (0 2 78± 0 0 99)nmol mgwettissue·min ,P <0 0 1 ],CSEmR NA的含量明显减少 [(1 0 2± 0 1 5 )× 1 0 - 6 fmolvs (2 1 7± 0 2 2 )× 1 0 - 6 fmol,P <0 0 1 ]。以上研究表明 。
To investigate the impact of hypoxia on endogenous hydrogen sulfide (H 2 S) system in rats lung tissue. Plasma level of H 2 S and H 2 S generating enzymes activity in homogenates of rat lung tissue was measured by biochemical methods. Cystathionine γ lyase (CSE) mRNA in lung tissues was determined by Quantitative Competitive RT PCR. The results showed that plasma level of H 2 S, the enzymatic activity of H 2 S generating enzymes and the amount of CSE mRNA in lung homogenates of rats in hypoxia group were all significantly decreased as compared with those in control group [(192 2±22 1)μmol/L vs (301 6±32 4)μmol/L, (0 127±0 023 ) vs (0 278±0 099)nmol/mg wet tissue·min, (1 02±0 15)×10 -6 fmol vs (2 17±0 22)×10 -6 fmol, respectively, all P <0 01]。These results suggested that the endogenous H 2 S system of rats was suppressed by hypoxia.
出处
《基础医学与临床》
CSCD
北大核心
2004年第2期145-148,共4页
Basic and Clinical Medicine
基金
国家自然科学基金 (30 0 70 796 )
国家重点基础性研究项目 (G2 0 0 0 5 6 90 5 )
北京市自然科学基金 (70 1 2 0 2 1 )
985北京大学心血管重大研究项目
关键词
低氧
肺动脉高压
大鼠
肺组织
硫化氢
hydrogen sulfide
hypoxia
pulmonary hypertension
cystathionine γ lyase
competitive RT PCR
