摘要
目的研究糖尿病大鼠肾脏内源性硫化氢(H2S)的两种合成酶胱硫醚-β-合成酶ystathionine-β-synthase CBS)和胱硫醚-γ-裂解酶(cystathionine-γ-lyase CSE)mRNA表达。方法用腹腔注射链脲佐菌素诱发糖尿病模型,大鼠随机分为两组:正常对照组(C组)和糖尿病组(D组)。建模第8周,测定24h尿蛋白、血糖及肌酐清除率[1],应用RT-PCR技术检测大鼠肾皮质CBS、CSE mRNA表。结果糖尿病组大鼠尿蛋白、血糖及肌酐清除率均较正常对照组明显升高(P<0.05),但肾皮质CBS、E mRNA表达较正常对照组无显著性差异(P>0.05)。结论CBS、CSE/H2S系统不能通过两种合成酶NA水平的改变而参与糖尿病肾病中血流动力学紊乱发生,其作用通路中的其他环节改变是否参与糖尿病病的发生发展尚需进一步证实。
Objective: To investigate the expression of enzyme cystathionine-β-synthase(CBS) and cystathionine-γ-lyase(CSE) at mRNA level generated by hydrogen sulfide (H2S) in renal cortex of diabetic rats. Methods: The male SD rats were randomly divided into two groups: control group and diabetic group. After eight weeks, when the model was established, the excretion of urine protein, the level of blood glucose and the clearance of creatinine were measured. The expression of CBS and CSE at mRNA level in renal cortex were detected by RT-PCR. Results: Urinary protein excretion, blood glucose and creatinine clearance increased in diabetic rats. But the expression of CBS and CSE at mRNA level had no significant differences in diabetic rats compared with those in control group. Conclusions: CBS, CSE/H2S couldn′t play a role in the hemodynamic disorder of diabetic nephropathy by the change of two synthase at mRNA level. It is needed to demonstrate whether the change of other levels of the CBS, CSE/H2S pathway can take part in the diabetic nephropathy pathogenesis.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第19期6-9,共4页
China Journal of Modern Medicine
